@misc{balk_lignin_the_2023, author={Balk, M., Sofia, P., Neffe, A.T., Tirelli, N.}, title={Lignin, the lignification process, and advanced, lignin-based materials}, year={2023}, howpublished = {journal article}, doi = {https://doi.org/10.3390/ijms241411668}, abstract = {At a time when environmental considerations are increasingly pushing for the application of circular economy concepts in materials science, lignin stands out as an under-used but promising and environmentally benign building block. This review focuses (A) on understanding what we mean with lignin, i.e., where it can be found and how it is produced in plants, devoting particular attention to the identity of lignols (including ferulates that are instrumental for integrating lignin with cell wall polysaccharides) and to the details of their coupling reactions and (B) on providing an overview how lignin can actually be employed as a component of materials in healthcare and energy applications, finally paying specific attention to the use of lignin in the development of organic shape-memory materials.}, note = {Online available at: \url{https://doi.org/10.3390/ijms241411668} (DOI). Balk, M.; Sofia, P.; Neffe, A.; Tirelli, N.: Lignin, the lignification process, and advanced, lignin-based materials. International Journal of Molecular Sciences. 2023. vol. 24, no. 14, 11668. DOI: 10.3390/ijms241411668}} @misc{kirchhecker_ironii_carboxylates_2023, author={Kirchhecker, S., Nguyen, N., Reichert, S., Lützow, K., Eselem Bungu, P.S., van Wangelin, A.J., Sandl, S., Neffe, A.T.}, title={Iron(II) Carboxylates and Simple Carboxamides: An Inexpensive and Modular Catalyst System for the Synthesis of PLLA and PLLA-PCL Block Copolymers;}, year={2023}, howpublished = {journal article}, doi = {https://doi.org/10.1039/d3ra03112h}, abstract = {The combination of inexpensive Fe(II) acetate with low molecular weight aliphatic carboxamides in situ generates an effective catalyst system for the ring opening polymerisation of lactones. PLLAs were produced in melt conditions with molar masses of up to 15 kg mol−1, narrow dispersity (Đ = 1.03), and without racemisation. The catalytic system was investigated in detail with regard to Fe(II) source, and steric and electronic effects of the amide's substituents. Furthermore, the synthesis of PLLA-PCL block copolymers of very low randomness was achieved. This commercially available, inexpensive, modular, and user-friendly catalyst mixture may be suitable for polymers with biomedical applications.}, note = {Online available at: \url{https://doi.org/10.1039/d3ra03112h} (DOI). Kirchhecker, S.; Nguyen, N.; Reichert, S.; Lützow, K.; Eselem Bungu, P.; van Wangelin, A.; Sandl, S.; Neffe, A.: Iron(II) Carboxylates and Simple Carboxamides: An Inexpensive and Modular Catalyst System for the Synthesis of PLLA and PLLA-PCL Block Copolymers;. RSC Advances. 2023. vol. 13, 17102-17113. DOI: 10.1039/d3ra03112h}} @misc{farhan_bioinspired_magnetically_2023, author={Farhan, M., Hartstein, D.S., Pieper, Y., Behl, M., Lendlein, A., Neffe, A.T.}, title={Bio-inspired magnetically controlled reversibly actuating multimaterial fibers}, year={2023}, howpublished = {journal article}, doi = {https://doi.org/10.3390/polym15092233}, abstract = {Movements in plants, such as the coiling of tendrils in climbing plants, have been studied as inspiration for coiling actuators in robotics. A promising approach to mimic this behavior is the use of multimaterial systems that show different elastic moduli. Here, we report on the development of magnetically controllable/triggerable multimaterial fibers (MMFs) as artificial tendrils, which can reversibly coil and uncoil on stimulation from an alternating magnetic field. These MMFs are based on deformed shape-memory fibers with poly[ethylene-co-(vinyl acetate)] (PEVA) as their core and a silicone-based soft elastomeric magnetic nanocomposite shell. The core fiber provides a temperature-dependent expansion/contraction that propagates the coiling of the MMF, while the shell enables inductive heating to actuate the movements in these MMFs. Composites with mNP weight content ≥ 15 wt% were required to achieve heating suitable to initiate movement. The MMFs coil upon application of the magnetic field, in which a degree of coiling N = 0.8 ± 0.2 was achieved. Cooling upon switching OFF the magnetic field reversed some of the coiling, giving a reversible change in coiling ∆n = 2 ± 0.5. These MMFs allow magnetically controlled remote and reversible actuation in artificial (soft) plant-like tendrils, and are envisioned as fiber actuators in future robotics applications.}, note = {Online available at: \url{https://doi.org/10.3390/polym15092233} (DOI). Farhan, M.; Hartstein, D.; Pieper, Y.; Behl, M.; Lendlein, A.; Neffe, A.: Bio-inspired magnetically controlled reversibly actuating multimaterial fibers. Polymers. 2023. vol. 15, no. 9, 2233. DOI: 10.3390/polym15092233}} @misc{neffe_cellmaterial_interactions_2023, author={Neffe, A.T.}, title={Cell-Material Interactions 2022}, year={2023}, howpublished = {Other: editorial}, doi = {https://doi.org/10.3390/ijms24076057}, note = {Online available at: \url{https://doi.org/10.3390/ijms24076057} (DOI). Neffe, A.: Cell-Material Interactions 2022. International Journal of Molecular Sciences. 2023. vol. 24, no. 7, 6057. DOI: 10.3390/ijms24076057}} @misc{folikumah_a_4arm_2023, author={Folikumah, M.Y., Behl, M., Lendlein, A., Neffe, A.T.}, title={A 4-Arm PEG-Thiodepsipeptide Precursor Enables Gelatinase-promoted Hydrogel Formation}, year={2023}, howpublished = {journal article}, doi = {https://doi.org/10.1002/mame.202300146}, abstract = {In situ hydrogelation of injectable precursors upon biological stimulus is relevant to generate hydrogels under mild conditions and, potentially, at a biological side of interest. Here, it is shown that hydrolytic enzymes can be used to initiate the formation of covalent hydrogel networks, realizing a cleavage-leading-to-gelation strategy. For this purpose, a two-component system is used, consisting of a 4-arm polyethylene glycol-thiodepsipeptide conjugate, PEG4TDPo containing the matrix metalloproteinases MMP-2- and MMP-9-cleavable Ac-Pro-Leu-Gly#SLeu-Leu-Gly- thiodepsipeptide sequence releasing a thiol upon hydrolysis, and a maleimide functionalized 4-armed PEG (PEG4MAL). PEG4TDPo is synthesized in a PEG-functionalization protocol involving convergent and divergent synthetic steps without the need for rigorous purification procedures. In a fluorometric assay, it is shown that the construct is in fact cleaved by both investigated MMPs. PEG4TDPo in the presence of 10 wt.% PEG4MAL formed hydrogels upon addition of MMP-2 or -9 with average gelation times of 28 and 40 min, respectively, as is investigated by rheology. The much faster gelation times compared to the enzyme-free system showed the specific input of the enzymatic reactions. The MMP-assisted activation and crosslinking strategy can potentially become useful by targeting tissues showing an increased expression of MMPs, such as cancers, or to detect MMPs.}, note = {Online available at: \url{https://doi.org/10.1002/mame.202300146} (DOI). Folikumah, M.; Behl, M.; Lendlein, A.; Neffe, A.: A 4-Arm PEG-Thiodepsipeptide Precursor Enables Gelatinase-promoted Hydrogel Formation. Macromolecular Materials and Engineering. 2023. vol. 308, no. 11, 2300146. DOI: 10.1002/mame.202300146}} @misc{tung_in_vivo_2022, author={Tung, W.T., Maring, J.A., Xu, X., Liu, Y., Becker, M., Somesh, D.B., Klose, K., Wang, W., Sun, X., Ullah, I., Kratz, K., Neffe, A.T., Stamm, C., Ma, N., Lendlein, A.}, title={In Vivo Performance of a Cell and Factor Free Multifunctional Fiber Mesh Modulating Postinfarct Myocardial Remodeling}, year={2022}, howpublished = {journal article}, doi = {https://doi.org/10.1002/adfm.202110179}, abstract = {Guidance of postinfarct myocardial remodeling processes by an epicardial patch system may alleviate the consequences of ischemic heart disease. As macrophages are highly relevant in balancing immune response and regenerative processes their suitable instruction would ensure therapeutic success. A polymeric mesh capable of attracting and instructing monocytes by purely physical cues and accelerating implant degradation at the cell/implant interface is designed. In a murine model for myocardial infarction the meshes are compared to those either coated with extracellular matrix or loaded with induced cardiomyocyte progenitor cells. All implants promote macrophage infiltration and polarization in the epicardium, which is verified by in vitro experiments. 6 weeks post-MI, especially the implantation of the mesh attenuates left ventricular adverse remodeling processes as shown by reduced infarct size (14.7% vs 28–32%) and increased wall thickness (854 µm vs 400–600 µm), enhanced angiogenesis/arteriogenesis (more than 50% increase compared to controls and other groups), and improved heart function (ejection fraction = 36.8% compared to 12.7–31.3%). Upscaling as well as process controls is comprehensively considered in the presented mesh fabrication scheme to warrant further progression from bench to bedside.}, note = {Online available at: \url{https://doi.org/10.1002/adfm.202110179} (DOI). Tung, W.; Maring, J.; Xu, X.; Liu, Y.; Becker, M.; Somesh, D.; Klose, K.; Wang, W.; Sun, X.; Ullah, I.; Kratz, K.; Neffe, A.; Stamm, C.; Ma, N.; Lendlein, A.: In Vivo Performance of a Cell and Factor Free Multifunctional Fiber Mesh Modulating Postinfarct Myocardial Remodeling. Advanced Functional Materials. 2022. vol. 32, no. 31, 2110179. DOI: 10.1002/adfm.202110179}} @misc{neffe_anstze_und_2022, author={Neffe, A.}, title={Ansätze und Fallstricke in der transferorientierten Forschung aus Sicht des Wissenschaftlers}, year={2022}, howpublished = {conference lecture (invited): Virtual;}, note = {Neffe, A.: Ansätze und Fallstricke in der transferorientierten Forschung aus Sicht des Wissenschaftlers. FGW Partnering Workshop 2022 : Biofunktionalisierung von Materialien in Medizin und Gesundheit. Virtual, 2022.}} @misc{neffe_hydrogel_networks_2021, author={Neffe, A., Löwenberg, C., Lendlein, A.}, title={Hydrogel networks by aliphatic dithiol Michael addition to glycidylmethacrylated gelatin}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.1557/s43580-021-00136-8}, abstract = {Functionalization of gelatin with glycidylmethacrylate (GMA-gelatin) enables network formation employing the double bond, so that the reaction is orthogonal to the inherent functional groups in the biomacromolecule. Here, network formation by crosslinking of GMA-gelatin with hexane 1,6-dithiol or nonane 1,9-dithiol to tailor properties and enable a shape-memory effect is shown by 1H NMR and FT-IR spectroscopy. Hydrogel swelling (460–1900 vol%) and mechanical properties (Young’s modulus E = 59–512 kPa, elongation at break εb = 44–127%) depended on the molecular composition of the networks and temperature. Increased crosslinker length, thiol:methacrylate molar ratio, and precursor concentrations led to denser networks. Change of properties with temperature suggested adoption of triple helices by gelatin chains, forming physical netpoints at lower temperatures (< 20 °C). However, the limited freedom of the gelatin chains to move allowed only a minimal extent of triple helices formation, as it became apparent from the related signal in wide-angle X-ray scattering and the thermal transition associated to triple helices in some networks by DSC. The presented strategy is likely transferable to other biomacromolecules, and the results suggest that too short crosslinkers may result in a significant amount of grafting rather than network formation.}, note = {Online available at: \url{https://doi.org/10.1557/s43580-021-00136-8} (DOI). Neffe, A.; Löwenberg, C.; Lendlein, A.: Hydrogel networks by aliphatic dithiol Michael addition to glycidylmethacrylated gelatin. MRS Advances. 2021. vol. 6, no. 33, 796-800. DOI: 10.1557/s43580-021-00136-8}} @misc{neffe_microparticles_from_2021, author={Neffe, A., Garcia Cruz, D., Roch, T., Lendlein, A.}, title={Microparticles from glycidylmethacrylated gelatin as cell carriers prepared in an aqueous two-phase system}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.eurpolymj.2020.110148}, abstract = {Encapsulation by polymeric biomaterials can provide mechanical protection of cells and shielding from the immune system of the host when implanted as cell therapy. At the same time, free exchange of nutrients and metabolites including bioactive molecules guiding regenerative processes is facilitated. Here, glycidylmethacrylated gelatin (GMA-gelatin) is explored as matrix material for adherent (L929 mouse fibroblasts) or non-adherent (Ramos blue) cells by an integrated process of shaping and chemical crosslinking. Microparticle formation was driven by a water-in-water-emulsion technique, which allowed simultaneous irradiation with light of 365 nm in the presence of the photosensitizer irgacure 2959. Suitable photopolymerization conditions were determined in experiments with GMA-gelatin and cells. More than 85% of the cells survived this procedure, and an encapsulation efficiency of up to 75 ± 2% was reached. Diffusivity of molecules up to a molar mass of 150 kg·mol−1 in the matrix was shown by the release of co-encapsulated FITC-labelled dextran. L929 as well as Ramos blue cells proliferated in the microparticle matrix after encapsulation and released enzymes that could be detected in the cell culture medium in an active form. L929 showed the ability to escape the particles over time. Altogether, the presented cell encapsulation system based on a material that is stable to hydrolytic degradation for several weeks is generally suitable for cell based therapy or in vitro test systems.}, note = {Online available at: \url{https://doi.org/10.1016/j.eurpolymj.2020.110148} (DOI). Neffe, A.; Garcia Cruz, D.; Roch, T.; Lendlein, A.: Microparticles from glycidylmethacrylated gelatin as cell carriers prepared in an aqueous two-phase system. European Polymer Journal. 2021. vol. 142, 110148. DOI: 10.1016/j.eurpolymj.2020.110148}} @misc{brunacci_formulation_of_2021, author={Brunacci, N., Wischke, C., Naolou, T., Patzelt, A., Lademann, J., Neffe, A., Lendlein, A.}, title={Formulation of drug-loaded oligodepsipeptide particles with submicron size}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-200977}, abstract = {The size of particulate carriers is key to their transport and distribution in biological systems, and needs to be tailored in the higher submicron range to enable follicular uptake for dermal treatment. Oligodepsipeptides are promising nanoparticulate carrier systems as they can be designed to exhibit enhanced interaction with drug molecules. Here, a fabrication scheme for drug-loaded submicron particles from oligo[3-(S)-sec-butylmorpholine-2,5-dione]diol (OBMD) is presented based on an emulsion solvent evaporation method with cosolvent, surfactant, and polymer concentration as variable process parameters. The particle size (300–950 nm) increased with lower surfactant concentration and higher oligomer concentration. The addition of acetone increased the particle size at low surfactant concentration. Particle size remained stable upon the encapsulation of models compounds dexamethasone (DXM) and Nile red (NR), having different physicochemical properties. DXM was released faster compared to NR due to its higher water solubility. Overall, the results indicated that both drug-loading and size control of OBMD submicron particles can be achieved. When applied on porcine ear skin samples, the NR-loaded particles have been shown to allow NR penetration into the hair follicle and the depth reached with the 300 nm particles was comparable to the one reached with the cream formulation. A potential benefit of the particles compared to a cream is their sustained release profile.}, note = {Online available at: \url{https://doi.org/10.3233/CH-200977} (DOI). Brunacci, N.; Wischke, C.; Naolou, T.; Patzelt, A.; Lademann, J.; Neffe, A.; Lendlein, A.: Formulation of drug-loaded oligodepsipeptide particles with submicron size. Clinical Hemorheology and Microcirculation. 2021. vol. 77, no. 2, 201-219. DOI: 10.3233/CH-200977}} @misc{neffe_thermallyinduced_shapememory_2021, author={Neffe, A., Löwenberg, C., Julich-Gruner, K., Behl, M., Lendlein, A.}, title={Thermally-Induced Shape-Memory Behavior of Degradable Gelatin-Based Networks}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.3390/ijms22115892}, abstract = {Shape-memory hydrogels (SMH) are multifunctional, actively-moving polymers of interest in biomedicine. In loosely crosslinked polymer networks, gelatin chains may form triple helices, which can act as temporary net points in SMH, depending on the presence of salts. Here, we show programming and initiation of the shape-memory effect of such networks based on a thermomechanical process compatible with the physiological environment. The SMH were synthesized by reaction of glycidylmethacrylated gelatin with oligo(ethylene glycol) (OEG) α,ω-dithiols of varying crosslinker length and amount. Triple helicalization of gelatin chains is shown directly by wide-angle X-ray scattering and indirectly via the mechanical behavior at different temperatures. The ability to form triple helices increased with the molar mass of the crosslinker. Hydrogels had storage moduli of 0.27–23 kPa and Young’s moduli of 215–360 kPa at 4 °C. The hydrogels were hydrolytically degradable, with full degradation to water-soluble products within one week at 37 °C and pH = 7.4. A thermally-induced shape-memory effect is demonstrated in bending as well as in compression tests, in which shape recovery with excellent shape-recovery rates Rr close to 100% were observed. In the future, the material presented here could be applied, e.g., as self-anchoring devices mechanically resembling the extracellular matrix.}, note = {Online available at: \url{https://doi.org/10.3390/ijms22115892} (DOI). Neffe, A.; Löwenberg, C.; Julich-Gruner, K.; Behl, M.; Lendlein, A.: Thermally-Induced Shape-Memory Behavior of Degradable Gelatin-Based Networks. International Journal of Molecular Sciences. 2021. vol. 22, no. 11, 5892. DOI: 10.3390/ijms22115892}} @misc{neffe_multifunctionality_by_2021, author={Neffe, A., Zhang, Q., Hommes-Schattmann, P., Wang, W., Xu, X., Ahmad, B., Williams, G., Lendlein, A.}, title={Multifunctionality by Core/Shell Design of PLLA/PDLA Nanofibres}, year={2021}, howpublished = {conference lecture: Virtual;}, note = {Neffe, A.; Zhang, Q.; Hommes-Schattmann, P.; Wang, W.; Xu, X.; Ahmad, B.; Williams, G.; Lendlein, A.: Multifunctionality by Core/Shell Design of PLLA/PDLA Nanofibres. Virtual MRS Spring Meeting. Virtual, 2021.}} @misc{neffe_soft_formstable_2021, author={Neffe, A., Izraylit, V., Hommes-Schattmann, P., Lendlein, A.}, title={Soft, Formstable (Co)Polyester Blend Elastomers}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.3390/nano11061472}, abstract = {High crystallization rate and thermomechanical stability make polylactide stereocomplexes effective nanosized physical netpoints. Here, we address the need for soft, form-stable degradable elastomers for medical applications by designing such blends from (co)polyesters, whose mechanical properties are ruled by their nanodimensional architecture and which are applied as single components in implants. By careful controlling of the copolymer composition and sequence structure of poly[(L-lactide)-co-(ε-caprolactone)], it is possible to prepare hyperelastic polymer blends formed through stereocomplexation by adding poly(D-lactide) (PDLA). Low glass transition temperature Tg ≤ 0 °C of the mixed amorphous phase contributes to the low Young’s modulus E. The formation of stereocomplexes is shown in DSC by melting transitions Tm > 190 °C and in WAXS by distinct scattering maxima at 2θ = 12° and 21°. Tensile testing demonstrated that the blends are soft (E = 12–80 MPa) and show an excellent hyperelastic recovery Rrec = 66–85% while having high elongation at break εb up to >1000%. These properties of the blends are attained only when the copolymer has 56–62 wt% lactide content, a weight average molar mass >140 kg·mol−1, and number average lactide sequence length ≥4.8, while the blend is formed with a content of 5–10 wt% of PDLA. The devised strategy to identify a suitable copolymer for stereocomplexation and blend formation is transferable to further polymer systems and will support the development of thermoplastic elastomers suitable for medical applications.}, note = {Online available at: \url{https://doi.org/10.3390/nano11061472} (DOI). Neffe, A.; Izraylit, V.; Hommes-Schattmann, P.; Lendlein, A.: Soft, Formstable (Co)Polyester Blend Elastomers. Nanomaterials. 2021. vol. 11, no. 6, 1472. DOI: 10.3390/nano11061472}} @misc{neffe_functionalizable_coaxial_2021, author={Neffe, A., Zhang, Q., Hommes-Schattmann, P., Wang, W., Xu, X., Ahmad, B., Williams, G., Lendlein, A.}, title={Functionalizable coaxial PLLA/PDLA nanofibers with stereocomplexes at the internal interface}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.1557/s43578-021-00260-z}, abstract = {Multifunctionality of electrospun polylactic acid (PLA) nonwovens was generated by the morphological design of nanofibers. Coaxial fibers with a lower number average molar mass Mn PLLA core and a higher Mn PDLA shell form PDLA–PLLA stereocrystals at the interface, induced by annealing. In tensile tests under physiological conditions, the core–shell fibers with higher crystallinity (22% compared to 11–14%) had lower Young’s moduli E (9 ± 1 MPa) and lower elongation at break εb (26 ± 3%) than PDLA alone (E = 31 ± 9 MPa, εb = 80 ± 5%), which can be attributed to simultaneous crystallization and relaxation effects. Gelatin incorporated in the PDLA phase was presented on the outer surface providing a biointerface putatively favorable for cell adherence. Gelatin incorporation did not influence the crystallization behavior but slightly lowered Tg (60 → 54 °C). Employing exclusively polymers established in the clinic, multifunctionality was generated by design.}, note = {Online available at: \url{https://doi.org/10.1557/s43578-021-00260-z} (DOI). Neffe, A.; Zhang, Q.; Hommes-Schattmann, P.; Wang, W.; Xu, X.; Ahmad, B.; Williams, G.; Lendlein, A.: Functionalizable coaxial PLLA/PDLA nanofibers with stereocomplexes at the internal interface. Journal of Materials Research. 2021. vol. 36, no. 14, 2995-3009. DOI: 10.1557/s43578-021-00260-z}} @misc{lau_establishment_of_2021, author={Lau, S., Liu, Y., Maier, A., Braune, S., Gossen, M., Neffe, A., Lendlein, A.}, title={Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.1557/s43579-021-00072-6}, abstract = {In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.}, note = {Online available at: \url{https://doi.org/10.1557/s43579-021-00072-6} (DOI). Lau, S.; Liu, Y.; Maier, A.; Braune, S.; Gossen, M.; Neffe, A.; Lendlein, A.: Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation. MRS Communications. 2021. vol. 11, no. 5, 559-567. DOI: 10.1557/s43579-021-00072-6}} @misc{neffe_ethylene_oxide_2021, author={Neffe, A., Zhang, Q., Hommes-Schattmann, P., Lendlein, A.}, title={Ethylene oxide sterilization of electrospun poly(l-lactide)/poly(d-lactide) core/shell nanofibers}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.1557/s43580-021-00058-5}, abstract = {The application of polymers in medicine requires sterilization while retaining material structure and properties. This demands detailed analysis, which we show exemplarily for the sterilization of PLLA/PDLA core–shell nanofibers with ethylene oxide (EtO). The electrospun patch was exposed to EtO gas (6 vol% in CO2, 1.7 bar) for 3 h at 45 °C and 75% rel. humidity, followed by degassing under pressure/vacuum cycles for 12 h. GC–MS analysis showed that no residual EtO was retained. Fiber diameters (~ 520 ± 130 nm) of the patches remained constant as observed by electron microscopy. Young’s modulus slightly increased and the elongation at break slightly decreased, determined at 37 °C. No changes were detected in 1H-NMR spectra, in molar mass distribution (GPC) or in crystallinity measured for annealed samples with comparable thermal history (Wide Angle X-Ray Scattering). Altogether, EtO emerged as suitable sterilization method for polylactide nanofibers with core–shell morphology.}, note = {Online available at: \url{https://doi.org/10.1557/s43580-021-00058-5} (DOI). Neffe, A.; Zhang, Q.; Hommes-Schattmann, P.; Lendlein, A.: Ethylene oxide sterilization of electrospun poly(l-lactide)/poly(d-lactide) core/shell nanofibers. MRS Advances. 2021. vol. 6, no. 33, 786-789. DOI: 10.1557/s43580-021-00058-5}} @misc{krgergenge_immunocompatibility_and_2021, author={Krüger-Genge, A., Tondera, C., Hauser, S., Braune, S., Görs, J., Roch, T., Klopfleisch, R., Neffe, A., Lendlein, A., Pietzsch, J., Jung, F.}, title={Immunocompatibility and non-thrombogenicity of gelatin-based hydrogels}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-201028}, abstract = {Immunocompatibility and non-thrombogenicity are important requirements for biomedical applications such as vascular grafts. Here, gelatin-based hydrogels formed by reaction of porcine gelatin with increasing amounts of lysine diisocyanate ethyl ester were investigated in vitro in this regard. In addition, potential adverse effects of the hydrogels were determined using the “Hen’s egg test on chorioallantoic membrane” (HET-CAM) test and a mouse model. The study revealed that the hydrogels were immunocompatible, since complement activation was absent and a substantial induction of reactive oxygen species generating monocytes and neutrophils could not be observed in whole human blood. The density as well as the activation state of adherent thrombocytes was comparable to medical grade polydimethylsiloxane, which was used as reference material. The HET-CAM test confirmed the compatibility of the hydrogels with vessel functionality since no bleedings, thrombotic events, or vessel destructions were observed. Only for the samples synthesized with the highest LDI amount the number of growing blood vessels in the CAM was comparable to controls and significantly higher than for the softer materials. Implantation into mice showed the absence of adverse or toxic effects in spleen, liver, or kidney, and only a mild lymphocytic activation in the form of a follicular hyperplasia in draining lymph nodes (slightly increased after the implantation of the material prepared with the lowest LDI content). These results imply that candidate materials prepared with mid to high amounts of LDI are suitable for the coating of the blood contacting surface of cardiovascular implants.}, note = {Online available at: \url{https://doi.org/10.3233/CH-201028} (DOI). Krüger-Genge, A.; Tondera, C.; Hauser, S.; Braune, S.; Görs, J.; Roch, T.; Klopfleisch, R.; Neffe, A.; Lendlein, A.; Pietzsch, J.; Jung, F.: Immunocompatibility and non-thrombogenicity of gelatin-based hydrogels. Clinical Hemorheology and Microcirculation. 2021. vol. 77, no. 3, 335-350. DOI: 10.3233/CH-201028}} @misc{krgergenge_response_of_2021, author={Krüger-Genge, A., Hauser, S., Neffe, A., Liu, Y., Lendlein, A., Pietzsch, J., Jung, F.}, title={Response of Endothelial Cells to Gelatin-Based Hydrogels}, year={2021}, howpublished = {journal article}, doi = {https://doi.org/10.1021/acsbiomaterials.0c01432}, abstract = {The establishment of confluent endothelial cell (EC) monolayers on implanted materials has been identified as a concept to avoid thrombus formation but is a continuous challenge in cardiovascular device engineering. Here, material properties of gelatin-based hydrogels obtained by reacting gelatin with varying amounts of lysine diisocyanate ethyl ester were correlated with the functional state of hydrogel contacting venous EC (HUVEC) and HUVEC’s ability to form a monolayer on these hydrogels. The density of adherent HUVEC on the softest hydrogel at 37 °C (G’ = 1.02 kPa, E = 1.1 ± 0.3 kPa) was significantly lower (125 mm–1) than on the stiffer hydrogels (920 mm–1; G’ = 2.515 and 5.02 kPa, E = 4.8 ± 0.8 and 10.3 ± 1.2 kPa). This was accompanied by increased matrix metalloprotease activity (9 pmol·min–2 compared to 0.6 pmol·min–2) and stress fiber formation, while cell-to-cell contacts were comparable. Likewise, release of eicosanoids (e.g., prostacyclin release of 1.7 vs 0.2 pg·mL–1·cell–1) and the pro-inflammatory cytokine MCP-1 (8 vs <1.5 pg·mL–1·cell–1) was higher on the softer than on the stiffer hydrogels. The expressions of pro-inflammatory markers COX-2, COX-1, and RAGE were slightly increased on all hydrogels on day 2 (up to 200% of the control), indicating a weak inflammation; however, the levels dropped to below the control from day 6. The study revealed that hydrogels with higher moduli approached the status of a functionally confluent HUVEC monolayer. The results indicate the promising potential especially of the discussed gelatin-based hydrogels with higher G’ as biomaterials for implants foreseen for the venous system.}, note = {Online available at: \url{https://doi.org/10.1021/acsbiomaterials.0c01432} (DOI). Krüger-Genge, A.; Hauser, S.; Neffe, A.; Liu, Y.; Lendlein, A.; Pietzsch, J.; Jung, F.: Response of Endothelial Cells to Gelatin-Based Hydrogels. ACS Biomaterials Science & Engineering. 2021. vol. 7, no. 2, 527-540. DOI: 10.1021/acsbiomaterials.0c01432}} @misc{izraylit_polyester_urethane_2020, author={Izraylit, V., Hommes-Schattmann, P., Neffe, A., Gould, O., Lendlein, A.}, title={Polyester urethane functionalizable through maleimide side-chains and cross-linkable by polylactide stereocomplexes}, year={2020}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.eurpolymj.2020.109916}, abstract = {Sustainable multifunctional alternatives to fossil-derived materials, which can be functionalized and are degradable, can be envisioned by combining naturally derived starting materials with an established polymer design concept. Modularity and chemical flexibility of polyester urethanes (PEU) enable the combination of segments bearing functionalizable moieties and the tailoring of the mechanical and thermal properties. In this work, a PEU multiblock structure was synthesized from naturally derived L-lysine diisocyanate ethyl ester (LDI), poly(L-lactide) diol (PLLA) and N-(2,3-dihydroxypropyl)-maleimide (MID) in a one-step reaction. A maleimide side-chain (MID) provided a reactive site for the catalyst-free coupling of thiols shown for L-cysteine with a yield of 94%. Physical cross-links were generated by blending the PEU with poly(D-lactide) (PDLA), upon which the PLLA segments of the PEU and the PDLA formed stereocomplexes. Stereocomplexation occurred spontaneously during solution casting and was investigated with WAXS and DSC. Stereocomplex crystallites were observed in the blends, while isotactic PLA crystallization was not observed. The presented material platform with tailorable mechanical properties by blending is of specific interest for engineering biointerfaces of implants or carrier systems for bioactive molecules.}, note = {Online available at: \url{https://doi.org/10.1016/j.eurpolymj.2020.109916} (DOI). Izraylit, V.; Hommes-Schattmann, P.; Neffe, A.; Gould, O.; Lendlein, A.: Polyester urethane functionalizable through maleimide side-chains and cross-linkable by polylactide stereocomplexes. European Polymer Journal. 2020. vol. 137, 109916. DOI: 10.1016/j.eurpolymj.2020.109916}} @misc{lwenberg_supramolecular_gelatin_2020, author={Löwenberg, C., Tripodo, G., Julich-Gruner, K., Neffe, A., Lendlein, A.}, title={Supramolecular Gelatin Networks Based on Inclusion Complexes}, year={2020}, howpublished = {journal article}, doi = {https://doi.org/10.1002/mabi.202000221}, abstract = {Hydrogel forming physical networks based on gelatin are an attractive approach toward multifunctional biomaterials with the option of reshaping, self‐healing, and stimuli‐sensitivity. However, it is challenging to design such gelatin‐based hydrogels to be stable at body temperature. Here, gelatin functionalized with desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT) side chains is crosslinked with cyclodextrin (CD) dimers under formation of inclusions complexes. The supramolecular networks displayed at room temperature decreased water uptake (200–600 wt% for DAT‐based systems, 200 wt% for DATT based systems), and increased storage moduli up to 25.6 kPa determined by rheology compared to DAT(T) gelatin. The gel–sol transition temperature increased from 33 up to 42 °C. The presented system that is completely based on natural building blocks may form the basis for materials that may potentially respond by dissolution or changes of properties to changes in environmental conditions or to the presence of CD guest molecules.}, note = {Online available at: \url{https://doi.org/10.1002/mabi.202000221} (DOI). Löwenberg, C.; Tripodo, G.; Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Supramolecular Gelatin Networks Based on Inclusion Complexes. Macromolecular Bioscience. 2020. vol. 20, no. 10, 2000221. DOI: 10.1002/mabi.202000221}} @misc{lwenberg_saltinduced_shapememory_2020, author={Löwenberg, C., Julich-Gruner, K., Neffe, A., Behl, M., Lendlein, A.}, title={Salt-Induced Shape-Memory Effect in Gelatin-Based Hydrogels}, year={2020}, howpublished = {journal article}, doi = {https://doi.org/10.1021/acs.biomac.9b01753}, abstract = {Hydrophilic biopolymers display a strong tendency for self-organization into stable secondary, tertiary, and quaternary structures in aqueous environments. These structures are sensitive to changes in external conditions, such as temperature, pH or ions/salts, which may lead to molecular and/or macroscopic transitions. Here, we report on biopolymer-based stimuli-sensitive switchable matrices showing a shape-memory function as an output being alternatively switched by two different input signals, such as environmental changes in salt concentration or temperature. This was realized by implementing a shape-memory function in hydrogels based on the coil-to-helix transition of protein chains in gelatin-based networks. The hydrogels exhibited mechanical properties similar to that of soft tissue (storage modulus G′ = 1–100 kPa) and high swelling capabilities (Q = 1000–3000 vol %). In these gelatin-based networks, the covalent netpoints defined the permanent shape while after deformation helicalization of the gelatin acted as reversible stimuli-sensitive switches providing additional crosslinks capable of fixing the deformed temporary shape. By using either chaotropic salts to suppress gelatin helicalization or kosmotropic salts to support conformational changes of gelatin toward a helical orientation, these additional crosslinks could be cleaved or formed. In bending experiments, the strain fixity (Rf) and strain recovery ratios (Rr) were determined. While Rf ranged from 65 to 95% and was depending on the network composition, Rr were independent of the hydrogel composition with values about 100%. In addition, Rf and Rr were independent of the type of chaotropic salt that was used in this study, showing equal Rf and Rr values for MgCl2, NaSCN, and Mg(SCN)2.}, note = {Online available at: \url{https://doi.org/10.1021/acs.biomac.9b01753} (DOI). Löwenberg, C.; Julich-Gruner, K.; Neffe, A.; Behl, M.; Lendlein, A.: Salt-Induced Shape-Memory Effect in Gelatin-Based Hydrogels. Biomacromolecules. 2020. vol. 21, no. 6, 2024-2031. DOI: 10.1021/acs.biomac.9b01753}} @misc{izraylit_alkynylfunctionalized_chainextended_2020, author={Izraylit, V., Hommes-Schattmann, P., Neffe, A., Gould, O., Lendlein, A.}, title={Alkynyl-functionalized chain-extended PCL for coupling to biological molecules}, year={2020}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.eurpolymj.2020.109908}, abstract = {Chemical functionalization of poly(ε-caprolactone) (PCL) enables a molecular integration of additional function. Here, we report an approach to incorporate reactive alkynyl side-groups by synthesizing a chain-extended PCL, where the reactive site is introduced through the covalently functionalizable chain extender 3-(prop-2-yn-1-yloxy)propane-1,2-diol (YPD). Chain-extended PCL with Mw of 101 to 385 kg·mol−1 were successfully synthesized in a one-pot reaction from PCL-diols with various molar masses, L-lysine ethyl ester diisocyanate (LDI) or trimethyl(hexamethylene)diisocyanate (TMDI), and YPD, in which the density of functionalizable groups and spacing between them can be controlled by the composition of the polymer. The employed diisocyanate compounds and YPD possess an asymmetric structure and form a non-crystallizable segment leaving the PCL crystallites to dominate the material’s mechanical properties. The mixed glass transition temperature Tg = −60 to −46 °C of the PCL/polyurethane amorphous phase maintains the synthesized materials in a highly elastic state at ambient and physiological conditions. Reaction conditions for covalent attachment in copper(I)-catalyzed azide-alkyne-cycloaddition reactions (CuAAC) in solution were optimized in a series of model reactions between the alkyne moieties of the chain-extended PCL and benzyl azide, reaching conversions over 95% of the alkyne moieties and with yields of up to 94% for the purified functionalized PCL. This methodology was applied for reaction with the azide-functionalized cell adhesion peptide GRGDS. The required modification of the peptide provides selectivity in the coupling reactions. The obtained results suggest that YPD could potentially be employed as versatile molecular unit for the creation of a variety of functionalizable polyesters as well as polyurethanes and polycarbonates offering efficient and selective click-reactions.}, note = {Online available at: \url{https://doi.org/10.1016/j.eurpolymj.2020.109908} (DOI). Izraylit, V.; Hommes-Schattmann, P.; Neffe, A.; Gould, O.; Lendlein, A.: Alkynyl-functionalized chain-extended PCL for coupling to biological molecules. European Polymer Journal. 2020. vol. 136, 109908. DOI: 10.1016/j.eurpolymj.2020.109908}} @misc{folikumah_thiol_michaeltype_2019, author={Folikumah, M., Neffe, A., Behl, M., Lendlein, A.}, title={Thiol Michael-Type Reactions of Optically Active Mercapto-Acids in Aqueous Medium}, year={2019}, howpublished = {journal article}, doi = {https://doi.org/10.1557/adv.2019.308}, abstract = {In model reactions were investigated the kinetics, the specificity and influence of stereochemistry of this reaction. We could show that only reactions involving SH-Leu yielded the expected thiol-Michael product. The inability of SH-Phe to react was attributed to the steric hindrance of the bulky phenyl group. In aqueous media, successful reaction using SH-Leu is thought to proceed via the sodium salt formed in-situ by the addition of NaOH solution, which was intented to aid the solubility of the mercapto-acid in water. Fast reaction rates and complete acrylate/maleimide conversion were only realized at pH 7.2 or higher suggesting the possible use of SH-Leu under physiological conditions for thiol Michael-type reactions. This method of in-situ formed alkali salts could be used as a fast approach to screen mercapto-acids for thio Michael-type reactions without the synthesis of their corresponding esters.}, note = {Online available at: \url{https://doi.org/10.1557/adv.2019.308} (DOI). Folikumah, M.; Neffe, A.; Behl, M.; Lendlein, A.: Thiol Michael-Type Reactions of Optically Active Mercapto-Acids in Aqueous Medium. MRS Advances. 2019. vol. 4, no. 46 - 47, 2515-2525. DOI: 10.1557/adv.2019.308}} @misc{naolou_amides_as_2019, author={Naolou, T., Lendlein, A., Neffe, A.T.}, title={Amides as Non-polymerizable Catalytic Adjuncts Enable the Ring-Opening Polymerization of Lactide With Ferrous Acetate Under Mild Conditions}, year={2019}, howpublished = {journal article}, doi = {https://doi.org/10.3389/fchem.2019.00346}, abstract = {Sn-based catalysts are effective in the ring-opening polymerization (ROP) but are toxic. Fe(OAc)2 used as an alternative catalyst is suitable for the ROP of lactide only at higher temperatures (>170°C), associated with racemization. In the ROP of ester and amide group containing morpholinediones with Fe(OAc)2 to polydepsipeptides at 135°C, ester bonds were selectively opened. Here, it was hypothesized that ROP of lactones is possible with Fe(OAc)2 when amides are present in the reactions mixture as Fe-ligands could increase the solubility and activity of the metal catalytic center. The ROP of lactide in the melt with Fe(OAc)2 is possible at temperatures as low as 105°C, in the presence of N-ethylacetamide or N-methylbenzamide as non-polymerizable catalytic adjuncts (NPCA), with high conversion (up to 99 mol%) and yield (up to 88 mol%). Polydispersities of polylactide decreased with decreasing reaction temperature to ≤ 1.1. NMR as well as polarimetric studies showed that no racemization occurred at reaction temperatures ≤145°C. A kinetic study demonstrated a living chain-growth mechanism. MALDI analysis revealed that no side reactions (e.g., cyclization) occurred, though transesterification took place.}, note = {Online available at: \url{https://doi.org/10.3389/fchem.2019.00346} (DOI). Naolou, T.; Lendlein, A.; Neffe, A.: Amides as Non-polymerizable Catalytic Adjuncts Enable the Ring-Opening Polymerization of Lactide With Ferrous Acetate Under Mild Conditions. Frontiers in Chemistry. 2019. vol. 7, 346. DOI: 10.3389/fchem.2019.00346}} @misc{hauser_characterization_of_2019, author={Hauser, S., Wodtke, R., Tondera, C., Wodtke, J., Neffe, A., Hampe, J., Lendlein, A., Löser, R., Pietzsch, J.}, title={Characterization of Tissue Transglutaminase as a Potential Biomarker for Tissue Response toward Biomaterials}, year={2019}, howpublished = {journal article}, doi = {https://doi.org/10.1021/acsbiomaterials.9b01299}, abstract = {Tissue transglutaminase (TGase 2) is proposed to be important for biomaterial–tissue interactions due to its presence and versatile functions in the extracellular environment. TGase 2 catalyzes the cross-linking of proteins through its Ca2+-dependent acyltransferase activity. Moreover, it enhances the interactions between fibronectin and integrins, which in turn mediates the adhesion, migration, and motility of the cells. TGase 2 is also a key player in the pathogenesis of fibrosis. In this study, we investigated whether TGase 2 is present at the biomaterial–tissue interface and might serve as an informative biomarker for the visualization of tissue response toward gelatin-based biomaterials. Two differently cross-linked hydrogels were used, which were obtained by the reaction of gelatin with lysine diisocyanate ethyl ester. The overall expression of TGase 2 by endothelial cells, macrophages, and granulocytes was partly influenced by contact to the hydrogels or their degradation products, although no clear correlation was evidenced. In contrast, the secretion of TGase 2 differed remarkably between the different cells, indicating that it might be involved in the cellular reaction toward gelatin-based hydrogels. The hydrogels were implanted subcutaneously in immunocompetent, hairless SKH1-Elite mice. Ex vivo immunohistochemical analysis of tissue sections over 112 days revealed enhanced expression of TGase 2 around the hydrogels, in particular at days 14 and 21 post-implantation. The incorporation of fluorescently labeled cadaverine derivatives for the detection of active TGase 2 was in accordance with the results of the expression analysis. The presence of an irreversible inhibitor of TGase 2 led to attenuated incorporation of the cadaverines, which verified the catalytic action of TGase 2. Our in vitro and ex vivo results verified TGase 2 as a potential biomarker for tissue response toward gelatin-based hydrogels. In vivo, no TGase 2 activity was detectable, which is mainly attributed to the unfavorable physicochemical properties of the cadaverine probe used.}, note = {Online available at: \url{https://doi.org/10.1021/acsbiomaterials.9b01299} (DOI). Hauser, S.; Wodtke, R.; Tondera, C.; Wodtke, J.; Neffe, A.; Hampe, J.; Lendlein, A.; Löser, R.; Pietzsch, J.: Characterization of Tissue Transglutaminase as a Potential Biomarker for Tissue Response toward Biomaterials. ACS Biomaterials Science & Engineering. 2019. vol. 5, no. 11, 5979-5989. DOI: 10.1021/acsbiomaterials.9b01299}} @misc{luetzow_perfluorophenyl_azide_2019, author={Luetzow, K., Hommes-Schattmann, P.J., Neffe, A.T., Ahmad, B., Williams, G.R., Lendlein, A.}, title={Perfluorophenyl azide functionalization of electrospun poly(para-dioxanone)}, year={2019}, howpublished = {journal article}, doi = {https://doi.org/10.1002/pat.4331}, abstract = {Strategies to surface‐functionalize scaffolds by covalent binding of biologically active compounds are of fundamental interest to control the interactions between scaffolds and biomolecules or cells. Poly(para‐dioxanone) (PPDO) is a clinically established polymer that has shown potential as temporary implant, eg, for the reconstruction of the inferior vena cava, as a nonwoven fiber mesh. However, PPDO lacks suitable chemical groups for covalent functionalization. Furthermore, PPDO is highly sensitive to hydrolysis, reflected by short in vivo half‐life times and degradation during storage. Establishing a method for covalent functionalization without degradation of this hydrolyzable polymer is therefore important to enable the surface tailoring for tissue engineering applications. It was hypothesized that treatment of PPDO with an N‐hydroxysuccinimide ester group bearing perfluorophenyl azide (PFPA) under UV irradiation would allow efficient surface functionalization of the scaffold. X‐ray photoelectron spectroscopy and attenuated total reflectance Fourier‐transformed infrared spectroscopy investigation revealed the successful binding, while a gel permeation chromatography study showed that degradation did not occur under these conditions. Coupling of a rhodamine dye to the N‐hydroxysuccinimide esters on the surface of a PFPA‐functionalized scaffold via its amine linker showed a homogenous staining of the PPDO in laser confocal microscopy. The PFPA method is therefore applicable even to the surface functionalization of hydrolytically labile polymers, and it was demonstrated that PFPA chemistry may serve as a versatile tool for the (bio‐)functionalization of PPDO scaffolds.}, note = {Online available at: \url{https://doi.org/10.1002/pat.4331} (DOI). Luetzow, K.; Hommes-Schattmann, P.; Neffe, A.; Ahmad, B.; Williams, G.; Lendlein, A.: Perfluorophenyl azide functionalization of electrospun poly(para-dioxanone). Polymers for Advanced Technologies. 2019. vol. 30, no. 5, 1165-1172. DOI: 10.1002/pat.4331}} @misc{folikumah_thiolmichael_reactions_2019, author={Folikumah, M., Neffe, A., Behl, M., Lendlein, A.}, title={Thiol-Michael reactions of optically-active mercapto-acids in aqueous medium}, year={2019}, howpublished = {conference poster: Espoo (FIN);}, note = {Folikumah, M.; Neffe, A.; Behl, M.; Lendlein, A.: Thiol-Michael reactions of optically-active mercapto-acids in aqueous medium. In: Advanced Functional Polymers for Medicine (AFPM) 2019. Espoo (FIN). 2019.}} @misc{brunacci_oligodepsipeptide_nanocarriers_2019, author={Brunacci, N., Neffe, A., Wischke, C., Naolou, T., Nöchel, U., Lendlein, A.}, title={Oligodepsipeptide (nano)carriers: Computational design and analysis of enhanced drug loading}, year={2019}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jconrel.2019.03.004}, abstract = {High drug loads of nanoparticles are essential to efficiently provide a desired dosage in the required timeframe, however, these conditions may not be reached with so far established degradable matrices. Our conceptual approach for increasing the drug load is based on strengthening the affinity between drug and matrix in combination with stabilizing drug-matrix-hybrids through strong intermolecular matrix interactions. Here, a method for designing such complex drug-matrix hybrids is introduced employing computational methods (molecular dynamics and docking) as well as experimental studies (affinity, drug loading and distribution, drug release from films and nanoparticles). As model system, dexamethasone (DXM), relevant for the treatment of inflammatory diseases, in combination with poly[(rac-lactide)-co-glycolide] (PLGA) as standard degradable matrix or oligo[(3-(S)-sec-butyl)morpholine-2,5-dione]diol (OBMD) as matrix with hypothesized stronger interaction with DXM were investigated. Docking studies predicted higher affinity of DXM to OBMD than PLGA and displayed amide bond participation in hydrogen bonding with OBMD. Experimental investigations on films and nanoparticles, i.e. matrices of different shapes and sizes, confirmed this phenomenon as shown e.g. by a ~10 times higher solid state solubility of DXM in OBMD than in PLGA. DXM-loaded particles of ~ 150 nm prepared by nanoprecipitation in aqueous environment had a drug loading (DL) up to 16 times higher when employing OBMD as matrix compared to PLGA carriers due to enhanced drug retention in the OBMD phase. Importantly, drug relase periods were not altered as the release from films and particles was mainly ruled by the diffusion length as well as matrix degradation rather than the matrix type, which can be assigned to water diffusing into the matrix and breaking up of drug-matrix hydrogen bonds. Overall, the presented design and fabrication scheme showed predictive power and might universally enable the screening of drug/matrix interactions particularly to expand the oligodepsipeptide platform technology, e.g. by varying the depsipeptide side chains, for drug carrier and release systems.}, note = {Online available at: \url{https://doi.org/10.1016/j.jconrel.2019.03.004} (DOI). Brunacci, N.; Neffe, A.; Wischke, C.; Naolou, T.; Nöchel, U.; Lendlein, A.: Oligodepsipeptide (nano)carriers: Computational design and analysis of enhanced drug loading. Journal of Controlled Release. 2019. vol. 301, 146-156. DOI: 10.1016/j.jconrel.2019.03.004}} @misc{folikumah_thiolmichael_reactions_2019, author={Folikumah, M., Neffe, A., Behl, M., Lendlein, A.}, title={Thiol-Michael reactions of optically active mercapto-acids in aqueous medium}, year={2019}, howpublished = {conference poster: Berlin (DEU);}, note = {Folikumah, M.; Neffe, A.; Behl, M.; Lendlein, A.: Thiol-Michael reactions of optically active mercapto-acids in aqueous medium. In: Polydays 2019 - Polymer Science and Engineering in View of Digitalization. Berlin (DEU). 2019.}} @misc{papagrigorakes_admscs_change_2018, author={Papagrigorakes, M., Chirico, N., Blocki, A., Neffe, A.T., Jung, F., Ma, N., Lendlein, A.}, title={AD-MSCS change their morphology and secretion profile as a response to changes in substrates`elastic properties in combination with inflammatory stimuli}, year={2018}, howpublished = {conference lecture: Krakau (PL);}, note = {Papagrigorakes, M.; Chirico, N.; Blocki, A.; Neffe, A.; Jung, F.; Ma, N.; Lendlein, A.: AD-MSCS change their morphology and secretion profile as a response to changes in substrates`elastic properties in combination with inflammatory stimuli. Joint Conference of Three Societies: European Society of Clinical Hemorheology and Miclrocirculation, International Society of Biorheology, International Society of Clinical Hemorheology, ESCHM-ISB-ISCH 2018. Krakau (PL), 2018.}} @misc{lendlein_depsipeptides__2018, author={Lendlein, A., Neffe, A.T.}, title={Depsipeptides - An innovative nanocarrier system}, year={2018}, howpublished = {conference lecture: Berlin (D);}, note = {Lendlein, A.; Neffe, A.: Depsipeptides - An innovative nanocarrier system. 2nd International Conference on Dermal Delivery by Nanocarriers: Highlights from SFB 1112, Abschlusscolloquium. Berlin (D), 2018.}} @misc{sarem_interplay_between_2018, author={Sarem, M., Arya, N., Heizmann, M., Neffe, A., Barbero, A., Gebauer, T., Martin, I., Lendlein, A., Shastri, V.}, title={Interplay between stiffness and degradation of architectured gelatin hydrogels leads to differential modulation of chondrogenesis in vitro and in vivo}, year={2018}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.actbio.2018.01.025}, abstract = {The limited capacity of cartilage to heal large lesions through endogenous mechanisms has led to extensive effort to develop materials to facilitate chondrogenesis. Although physical-chemical properties of biomaterials have been shown to impact in vitro chondrogenesis, whether these findings are translatable in vivo is subject of debate. Herein, architectured 3D hydrogel scaffolds (ArcGel) (produced by crosslinking gelatin with ethyl lysine diisocyanate (LDI)) were used as a model system to investigate the interplay between scaffold mechanical properties and degradation on matrix deposition by human articular chondrocytes (HAC) from healthy donors in vitro and in vivo. Using ArcGel scaffolds of different tensile and shear modulus, and degradation behavior; in this study, we compared the fate of ex vivo engineered ArcGels-chondrocytes constructs, i.e. the traditional tissue engineering approach, with the de novo formation of cartilaginous tissue in HAC laden ArcGels in an ectopic nude mouse model. While the softer and fast degrading ArcGel (LNCO3) was more efficient at promoting chondrogenic differentiation in vitro, upon ectopic implantation, the stiffer and slow degrading ArcGel (LNCO8) was superior in maintaining chondrogenic phenotype in HAC and retention of cartilaginous matrix. Furthermore, surprisingly the de novo formation of cartilage tissue was promoted only in LNCO8. Since HAC cultured for only three days in the LNCO8 environment showed upregulation of hypoxia-associated genes, this suggests a potential role for hypoxia in the observed in vivo outcomes. In summary, this study sheds light on how immediate environment (in vivo versus in vitro) can significantly impact the outcomes of cell-laden biomaterials.}, note = {Online available at: \url{https://doi.org/10.1016/j.actbio.2018.01.025} (DOI). Sarem, M.; Arya, N.; Heizmann, M.; Neffe, A.; Barbero, A.; Gebauer, T.; Martin, I.; Lendlein, A.; Shastri, V.: Interplay between stiffness and degradation of architectured gelatin hydrogels leads to differential modulation of chondrogenesis in vitro and in vivo. Acta Biomaterialia. 2018. vol. 69, 83-94. DOI: 10.1016/j.actbio.2018.01.025}} @misc{piluso_sequential_alkyneazide_2018, author={Piluso, S., Vukicevic, R., Noechel, U., Braune, S., Lendlein, A., Neffe, A.}, title={Sequential alkyne-azide cycloadditions for functionalized gelatin hydrogel formation}, year={2018}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.eurpolymj.2018.01.017}, abstract = {While click chemistry reactions for biopolymer network formation are attractive as the defined reactions may allow good control of the network formation and enable subsequent functionalization, tailoring of gelatin network properties over a wide range of mechanical properties has yet to be shown. Here, it is demonstrated that copper-catalyzed alkyne-azide cycloaddition of alkyne functionalized gelatin with diazides gave hydrogel networks with properties tailorable by the ratio of diazide to gelatin and diazide rigidity. 4,4′-diazido-2,2′-stilbenedisulfonic acid, which has been used as rigid crosslinker, yielded hydrogels with Young’s moduli E of 50–390 kPa and swelling degrees Q of 150–250 vol.%, while the more flexible 1,8-diazidooctane resulted in hydrogels with E = 125–280 kPa and Q = 225–470 vol.%. Storage moduli could be varied by two orders of magnitude (G′ = 100–20,000 Pa). An indirect cytotoxicity test did not show cytotoxic properties. Even when employing 1:1 ratios of alkyne and azide moieties, the hydrogels were shown to contain both, unreacted alkyne groups on the gelatin backbone as well as dangling chains carrying azide groups as shown by reaction with functionalized fluorescein. The free groups, which can be tailored by the employed ratio of the reactants, are accessible for covalent attachment of drugs, as was demonstrated by functionalization with dexamethasone. The sequential network formation and functionalization with click chemistry allows access to multifunctional materials relevant for medical applications.}, note = {Online available at: \url{https://doi.org/10.1016/j.eurpolymj.2018.01.017} (DOI). Piluso, S.; Vukicevic, R.; Noechel, U.; Braune, S.; Lendlein, A.; Neffe, A.: Sequential alkyne-azide cycloadditions for functionalized gelatin hydrogel formation. European Polymer Journal. 2018. vol. 100, 77-85. DOI: 10.1016/j.eurpolymj.2018.01.017}} @misc{blocki_response_of_2017, author={Blocki, A., Loewenberg, C., Jiang, Y., Kratz, K., Neffe, A.T., Jung, F., Lendlein, A.}, title={Response of encapsulated cells to a gelatin matrix with varied bulk and microenvironmental elastic properties}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1002/pat.3947}, abstract = {Gelatin-based hydrogels offer various biochemical cues that support encapsulated cells and are therefore suitable as cell delivery vehicles in regenerative medicine. However, besides the biochemical signals, biomechanical cues are crucial to ensure an optimal support of encapsulated cells. Hence, we aimed to correlate the cellular response of encapsulated cells to macroscopic and microscopic elastic properties of glycidylmethacrylate (GMA)-functionalized gelatin-based hydrogels. To ensure that different observations in cellular behavior could be attributed to differences in elastic properties, an identical concentration as well as degree of functionalization of biopolymers was utilized to form covalently crosslinked hydrogels. Elastic properties were merely altered by varying the average gelatin-chain length. Hydrogels exhibited an increased degree of swelling and a decreased bulk elastic modulus G′ with prolonged autoclaving of the starting solution. This was accompanied by an increase of hydrogel mesh size and thus by a reduction of crosslinking density. Tougher hydrogels retained the largest amount of cells; however, they also interfered with cell viability. Softer gels contained a lower cell density, but supported cell elongation and viability. Observed differences could be partially attributed to differences in bulk properties, as high crosslinking densities interfere with diffusion and cell spreading and thus can impede cell viability. Interestingly, a microscopic elastic modulus in the range of native soft tissue supported cell viability and elongation best while ensuring a good cell entrapment. In conclusion, gelatin-based hydrogels providing a soft tissue-like microenvironment represent adequate cell delivery vehicles for tissue engineering approaches.}, note = {Online available at: \url{https://doi.org/10.1002/pat.3947} (DOI). Blocki, A.; Loewenberg, C.; Jiang, Y.; Kratz, K.; Neffe, A.; Jung, F.; Lendlein, A.: Response of encapsulated cells to a gelatin matrix with varied bulk and microenvironmental elastic properties. Polymers for Advanced Technologies. 2017. vol. 28, no. 10, 1245-1251. DOI: 10.1002/pat.3947}} @misc{zhang_polyacrylonitrileconvinyl_pyrrolidone_2017, author={Zhang, N., Said, A., Wischke, C., Kral, V., Brodwolf, R., Volz, P., Boreham, A., Gerecke, C., Li, W., Neffe, A.T., Kleuser, B., Alexiev, U., Lendlein, A., Schaefer-Korting, M.}, title={Poly[acrylonitrile-co-(N-vinyl pyrrolidone)] nanoparticles – Composition-dependent skin penetration enhancement of a dye probe and biocompatibility}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.ejpb.2016.10.019}, abstract = {Nanoparticles can improve topical drug delivery: size, surface properties and flexibility of polymer nanoparticles are defining its interaction with the skin. Only few studies have explored skin penetration for one series of structurally related polymer particles with systematic alteration of material composition. Here, a series of rigid poly[acrylonitrile-co-(N-vinyl pyrrolidone)] model nanoparticles stably loaded with Nile Red or Rhodamin B, respectively, was comprehensively studied for biocompatibility and functionality. Surface properties were altered by varying the molar content of hydrophilic NVP from 0 to 24.1% and particle size ranged from 35 to 244 nm. Whereas irritancy and genotoxicity were not revealed, lipophilic and hydrophilic nanoparticles taken up by keratinocytes affected cell viability. Skin absorption of the particles into viable skin ex vivo was studied using Nile Red as fluorescent probe. Whilst an intact stratum corneum efficiently prevented penetration, almost complete removal of the horny layer allowed nanoparticles of smaller size and hydrophilic particles to penetrate into viable epidermis and dermis.}, note = {Online available at: \url{https://doi.org/10.1016/j.ejpb.2016.10.019} (DOI). Zhang, N.; Said, A.; Wischke, C.; Kral, V.; Brodwolf, R.; Volz, P.; Boreham, A.; Gerecke, C.; Li, W.; Neffe, A.; Kleuser, B.; Alexiev, U.; Lendlein, A.; Schaefer-Korting, M.: Poly[acrylonitrile-co-(N-vinyl pyrrolidone)] nanoparticles – Composition-dependent skin penetration enhancement of a dye probe and biocompatibility. European Journal of Pharmaceutics and Biopharmaceutics. 2017. vol. 116, 66-75. DOI: 10.1016/j.ejpb.2016.10.019}} @misc{wang_polydepsipeptide_blockstabilized_2017, author={Wang, W., Naolou, T., Ma, N., Deng, Z., Xu, X., Mansfeld, U., Wischke, C., Gossen, M., Neffe, A.T., Lendlein, A.}, title={Polydepsipeptide Block-Stabilized Polyplexes for Efficient Transfection of Primary Human Cells}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1021/acs.biomac.7b01034}, abstract = {The rational design of a polyplex gene carrier aims to balance maximal effectiveness of nucleic acid transfection into cells with minimal adverse effects. Depsipeptide blocks with an Mn ∼ 5 kDa exhibiting strong physical interactions were conjugated with PEI moieties (2.5 or 10 kDa) to di- and triblock copolymers. Upon nanoparticle formation and complexation with DNA, the resulting polyplexes (sizes typically 60–150 nm) showed remarkable stability compared to PEI-only or lipoplex and facilitated efficient gene delivery. Intracellular trafficking was visualized by observing fluorescence-labeled pDNA and highlighted the effective cytoplasmic uptake of polyplexes and release of DNA to the perinuclear space. Specifically, a triblock copolymer with a middle depsipeptide block and two 10 kDa PEI swallowtail structures mediated the highest levels of transgenic VEGF secretion in mesenchymal stem cells with low cytotoxicity. These nanocarriers form the basis for a delivery platform technology, especially for gene transfer to primary human cells.}, note = {Online available at: \url{https://doi.org/10.1021/acs.biomac.7b01034} (DOI). Wang, W.; Naolou, T.; Ma, N.; Deng, Z.; Xu, X.; Mansfeld, U.; Wischke, C.; Gossen, M.; Neffe, A.; Lendlein, A.: Polydepsipeptide Block-Stabilized Polyplexes for Efficient Transfection of Primary Human Cells. Biomacromolecules. 2017. vol. 18, no. 11, 3819-3833. DOI: 10.1021/acs.biomac.7b01034}} @misc{blocki_engineering_of_2017, author={Blocki, A., Loeper, F., Chirico, N., Neffe, A.T., Jung, F., Stamm, C., Lendlein, A.}, title={Engineering of cell-laden gelatin-based microcapsules for cell delivery and immobilization in regenerative therapies}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-179206}, abstract = {10 wt% GMA-gelatin-based hydrogels with E moduli properties comparable to the native cellular niche proved to be a promising biomaterial suitable for the production of cell-laden microcapsules and shall be evaluated further for biomedical application.}, note = {Online available at: \url{https://doi.org/10.3233/CH-179206} (DOI). Blocki, A.; Loeper, F.; Chirico, N.; Neffe, A.; Jung, F.; Stamm, C.; Lendlein, A.: Engineering of cell-laden gelatin-based microcapsules for cell delivery and immobilization in regenerative therapies. Clinical Hemorheology and Microcirculation. 2017. vol. 67, no. 3-4, 251-259. DOI: 10.3233/CH-179206}} @misc{piluso_enzymatic_action_2017, author={Piluso, S., Lendlein, A., Neffe, A.T.}, title={Enzymatic action as switch of bulk to surface degradation of clicked gelatin-based networks}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1002/pat.3962}, abstract = {Polymer degradation occurs under physiological conditions in vitro and in vivo, especially when bonds susceptible to hydrolysis are present in the polymer. Understanding of the degradation mechanism, changes of material properties over time, and overall rate of degradation is a necessary prerequisite for the knowledge-based design of polymers with applications in biomedicine. Here, hydrolytic degradation studies of gelatin-based networks synthesized by copper-catalyzed azide-alkyne cycloaddition reaction are reported, which were performed with or without addition of an enzyme. In all cases, networks with a stilbene as crosslinker proofed to be more resistant to degradation than when an octyl diazide was used. Without addition of an enzyme, the rate of degradation was ruled by the crosslinking density of the network and proceeded via a bulk degradation mechanism. Addition of Clostridium histolyticum collagenase resulted in a much enhanced rate of degradation, which furthermore occurred via surface erosion. The mesh size of the hydrogels (>7 nm) was in all cases larger than the hydrodynamic radius of the enzyme (4.5 nm) so that even in very hydrophilic networks with large mesh size enzymes may be used to induce a fast surface degradation mechanism. This observation is of general interest when designing hydrogels to be applied in the presence of enzymes, as the degradation mechanism and material performance are closely interlinked.}, note = {Online available at: \url{https://doi.org/10.1002/pat.3962} (DOI). Piluso, S.; Lendlein, A.; Neffe, A.: Enzymatic action as switch of bulk to surface degradation of clicked gelatin-based networks. Polymers for Advanced Technologies. 2017. vol. 28, no. 10, 1318-1324. DOI: 10.1002/pat.3962}} @misc{blocki_engineering_of_2017, author={Blocki, A., Loeper, F., Chirico, N., Neffe, A.T., Jung, F., Stamm, C., Lendlein, A.}, title={Engineering of cell-laden gelatin-based microcapsules for cell delivery and immobilization in regenerative therapies}, year={2017}, howpublished = {conference lecture: Greifswald (D);}, note = {Blocki, A.; Loeper, F.; Chirico, N.; Neffe, A.; Jung, F.; Stamm, C.; Lendlein, A.: Engineering of cell-laden gelatin-based microcapsules for cell delivery and immobilization in regenerative therapies. 36th Conference of the German Society for Clinical Microcirculation and Hemorheology. Greifswald (D), 2017.}} @misc{lohmann_bone_regeneration_2017, author={Lohmann, P., Willuweit, A., Neffe, A.T., Geisler, S., Gebauer, T.P., Beer, S., Coenen, H.H., Fischer, H., Hermanns-Sachweh, B., Lendlein, A., Shah, N.J., Kiessling, F., Langen, K.-J.}, title={Bone regeneration induced by a 3D architectured hydrogel in a rat critical-size calvarial defect}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.biomaterials.2016.10.039}, abstract = {Bone regeneration can be stimulated by implantation of biomaterials, which is especially important for larger bone defects. Here, healing potency of the porous ArcGel was evaluated in a critical-size calvarial bone defect in rats in comparison with clinical standard autologous bone and Bio-Oss® Collagen (BioOss), a bone graft material frequently used in clinics. Bone healing and metabolic processes involved were monitored longitudinally by [18F]-fluoride and [18F]-FDG μ-PET/CT 1d, 3d, 3w, 6w, and 12w post implantation. Differences in quality of bone healing were assessed by ex vivo μ-CT, mechanical tests and histomorphometry. The amount of bone formed after implantation of ArcGel was comparable to autologous bone and superior to BioOss (histomorphometry). Furthermore, microarchitecture of newly formed bone was more physiological and better functional in case of ArcGel (push-out tests). [18F]-FDG uptake increased until 3d after implantation, and decreased until 12w for both ArcGel and BioOss. [18F]-fluoride uptake increased until 3w post implantation for all materials, but persisted significantly longer at higher levels for BioOss, which indicates a prolonged remodelling phase. The study demonstrates the potential of ArcGel to induce restitutio ad integrum comparable with clinical standard autologous bone and better bone regeneration in large defects compared to a commercial state-of-the-art biomaterial.}, note = {Online available at: \url{https://doi.org/10.1016/j.biomaterials.2016.10.039} (DOI). Lohmann, P.; Willuweit, A.; Neffe, A.; Geisler, S.; Gebauer, T.; Beer, S.; Coenen, H.; Fischer, H.; Hermanns-Sachweh, B.; Lendlein, A.; Shah, N.; Kiessling, F.; Langen, K.: Bone regeneration induced by a 3D architectured hydrogel in a rat critical-size calvarial defect. Biomaterials. 2017. vol. 113, 158-169. DOI: 10.1016/j.biomaterials.2016.10.039}} @misc{lendlein_shapememory_polymers_2017, author={Lendlein, A., Razzaq, M.Y., Wischke, C., Kratz, K., Heuchel, M., Zotzmann, J., Hiebl, B., Neffe, A.T., Behl, M.}, title={Shape-Memory Polymers}, year={2017}, howpublished = {book part}, doi = {https://doi.org/10.1016/B978-0-12-803581-8.10213-9}, abstract = {Medical devices such as implants, surgical instruments, extracorporal devices, and wound covers, as well as controlled drug delivery systems (CDDS) require a specific combination of material properties and functions including, for example, mechanical stability, biocompatibility, and biofunctionality. Polymeric biomaterials are of high relevance for such applications, as properties and functions can be tuned in a wide range by only small defined variations of their chemical or morphological structure. The rapid progress in surgical techniques, especially in minimally invasive surgery, requires smart materials, which are capable of an active on-demand movement and which do not need to be removed in a second surgery. These challenges can be addressed by shape-memory polymers (SMPs) described in this chapter. SMPs are of high technological significance for biomedical applications as they enable on-demand predefined changes in the shape of a device upon exposure to a suitable stimulus such as heat or alternating magnetic field (AMF). Multifunctional materials are obtained when the shape-memory effect is combined with an additional function such as hydrolytic degradability, biofunctionality, and controlled drug release. Selected biomaterials with shape-memory capability are presented, including data on their biocompatibility. The potential of SMPs as a platform technology for biomedical applications is sketched by an overview on SMP-based medical devices being developed and the potential use of SMPs as matrix for CDDS.}, note = {Online available at: \url{https://doi.org/10.1016/B978-0-12-803581-8.10213-9} (DOI). Lendlein, A.; Razzaq, M.; Wischke, C.; Kratz, K.; Heuchel, M.; Zotzmann, J.; Hiebl, B.; Neffe, A.; Behl, M.: Shape-Memory Polymers. In: Ducheyne, P.; Healy, K.; Hutmacher, D.; Grainger, D.; Kirkpatrick, C. (Ed.): Comprehensive Biomaterials II - Reference Module in Materials Science and Materials Engineering, Metallic, Ceramic, and Polymeric Biomaterials. Elsevier. 2017. 620-647. DOI: 10.1016/B978-0-12-803581-8.10213-9}} @misc{lendlein_wahlpflicht_polymerchemie_2017, author={Lendlein, A., Schroeter, M., Schulz, B., Neffe, A.T.}, title={Wahlpflicht Polymerchemie – Anwendungen in der Medizin Resorbierbare Polymere}, year={2017}, howpublished = {lecture: Universitaet Potsdam, FB Chemie;}, note = {Lendlein, A.; Schroeter, M.; Schulz, B.; Neffe, A.: Wahlpflicht Polymerchemie – Anwendungen in der Medizin Resorbierbare Polymere. Universitaet Potsdam, FB Chemie, 2017.}} @misc{brunacci_influence_of_2017, author={Brunacci, N., Wischke, C., Naolou, T., Neffe, A.T., Lendlein, A.}, title={Influence of surfactants on depsipeptide submicron particle formation}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.ejpb.2016.11.011}, abstract = {Surfactants are required for the formation and stabilization of hydrophobic polymeric particles in aqueous environment. In order to form submicron particles of varying sizes from oligo[3-(S)-sec-butylmorpholine-2,5-dione]diols ((OBMD)-diol), different surfactants were investigated. As new surfactants, four-armed star-shaped oligo(ethylene glycol)s of molecular weights of 5–20 kDa functionalized with desamino-tyrosine (sOEG-DAT) resulted in smaller particles with lower PDI than with desaminotyrosyl tyrosine (sOEG-DATT) in an emulsion/solvent evaporation method. In a second set of experiments, sOEG-DAT of Mn = 10 kDa was compared with the commonly employed emulsifiers polyvinylalcohol (PVA), polyoxyethylene (20) sorbitan monolaurate (Tween 20), and D-α-tocopherol polyethylene glycol succinate (VIT E-TPGS) for OBMD particle preparation. sOEG-DAT allowed to systematically change sizes in a range of 300 up to 900 nm with narrow polydispersity, while in the other cases, a lower size range (250–400 nm, PVA; ∼300 nm, Tween 20) or no effective particle formation was observed. The ability of tailoring particle size in a broad range makes sOEG-DAT of particular interest for the formation of oligodepsipeptide particles, which can further be investigated as drug carriers for controlled delivery.}, note = {Online available at: \url{https://doi.org/10.1016/j.ejpb.2016.11.011} (DOI). Brunacci, N.; Wischke, C.; Naolou, T.; Neffe, A.; Lendlein, A.: Influence of surfactants on depsipeptide submicron particle formation. European Journal of Pharmaceutics and Biopharmaceutics. 2017. vol. 116, 61-65. DOI: 10.1016/j.ejpb.2016.11.011}} @misc{hommesschattmann_rgd_constructs_2017, author={Hommes-Schattmann, P.J., Neffe, A.T., Ahmad, B., Williams, G.R., M´Bele, G., Vanneaux, V., Menasche, P., Kalfa, D., Lendlein, A.}, title={RGD constructs with physical anchor groups as polymer co-electrospinnable cell adhesives}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.1002/pat.3963}, abstract = {The tissue integration of synthetic polymers can be promoted by displaying RGD peptides at the biointerface with the objective of enhancing colonization of the material by endogenous cells. A firm but flexible attachment of the peptide to the polymer matrix, still allowing interaction with receptors, is therefore of interest. Here, the covalent coupling of flexible physical anchor groups, allowing for temporary immobilization on polymeric surfaces via hydrophobic or dipole–dipole interactions, to a RGD peptide was investigated. For this purpose, a stearate or an oligo(ethylene glycol) (OEG) was attached to GRGDS in 51–69% yield. The obtained RGD linker constructs were characterized by NMR, IR and MALDI-ToF mass spectrometry, revealing that the commercially available OEG and stearate linkers are in fact mixtures of similar compounds. The RGD linker constructs were co-electrospun with poly(p-dioxanone) (PPDO). After electrospinning, nitrogen could be detected on the surface of the PPDO fibers by X-ray photoelectron spectroscopy. The nitrogen content exceeded the calculated value for the homogeneous material mixture suggesting a pronounced presentation of the peptide on the fiber surface. Increasing amounts of RGD linker constructs in the electrospinning solution did not lead to a detection of an increased amount of peptide on the scaffold surface, suggesting inhomogeneous distribution of the peptide on the PPDO fiber surface. Human adipose-derived stem cells cultured on the patches showed similar viability as when cultured on PPDO containing pristine RGD. The fully characterized RGD linker constructs could serve as valuable tools for the further development of tissue-integrating polymeric scaffolds.}, note = {Online available at: \url{https://doi.org/10.1002/pat.3963} (DOI). Hommes-Schattmann, P.; Neffe, A.; Ahmad, B.; Williams, G.; M´Bele, G.; Vanneaux, V.; Menasche, P.; Kalfa, D.; Lendlein, A.: RGD constructs with physical anchor groups as polymer co-electrospinnable cell adhesives. Polymers for Advanced Technologies. 2017. vol. 28, no. 10, 1312-1317. DOI: 10.1002/pat.3963}} @misc{brunacci_evaluation_of_2017, author={Brunacci, N., Wischke, C., Neffe, A., Lendlein, A.}, title={Evaluation of surfactants for the formation of sub-micron depsipeptide particles}, year={2017}, howpublished = {conference lecture: Karlsruhe (DEU);}, note = {Brunacci, N.; Wischke, C.; Neffe, A.; Lendlein, A.: Evaluation of surfactants for the formation of sub-micron depsipeptide particles. BIFTM PhD Symposium - BioInterfaces in Technology and Medicine. Karlsruhe (DEU), 2017.}} @misc{zou_adipogenic_differentiation_2017, author={Zou, J., Wang, W., Neffe, A.T., Xu, X., Li, Z., Deng, Z., Sun, X. Ma, N., Lendlein, A.}, title={Adipogenic differentiation of human adipose derived mesenchymal stem cells in 3D architectured gelatin based hydrogels (ArcGel)}, year={2017}, howpublished = {conference lecture: Greifswald (D);}, note = {Zou, J.; Wang, W.; Neffe, A.; Xu, X.; Li, Z.; Deng, Z.; Sun, X.; Lendlein, A.: Adipogenic differentiation of human adipose derived mesenchymal stem cells in 3D architectured gelatin based hydrogels (ArcGel). 36th Conference of the German Society for Clinical Microcirculation and Hemorheology. Greifswald (D), 2017.}} @misc{zou_adipogenic_differentiation_2017, author={Zou, J., Wang, W., Neffe, A.T., Xu, X., Li, Z., Deng, Z., Sun, X. Ma, N., Lendlein, A.}, title={Adipogenic differentiation of human adipose derived mesenchymal stem cells in 3D architectured gelatin based hydrogels (ArcGel)}, year={2017}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-179210}, abstract = {Polymeric matrices mimicking multiple functions of the ECM are expected to enable a material induced regeneration of tissues. Here, we investigated the adipogenic differentiation of human adipose derived mesenchymal stem cells (hADSCs) in a 3D architectured gelatin based hydrogel (ArcGel) prepared from gelatin and L-lysine diisocyanate ethyl ester (LDI) in an one-step process, in which the formation of an open porous morphology and the chemical network formation were integrated. The ArcGel was designed to support adipose tissue regeneration with its 3D porous structure, high cell biocompatibility, and mechanical properties compatible with human subcutaneous adipose tissue. The ArcGel could support initial cell adhesion and survival of hADSCs. Under static culture condition, the cells could migrate into the inner part of the scaffold with a depth of 840±120 μm after 4 days, and distributed in the whole scaffold (2 mm in thickness) within 14 days. The cells proliferated in the scaffold and the fold increase of cell number after 7 days of culture was 2.55±0.08. The apoptotic rate of hADSCs in the scaffold was similar to that of cells maintained on tissue culture plates. When cultured in adipogenic induction medium, the hADSCs in the scaffold differentiated into adipocytes with a high efficiency (93±1%). Conclusively, this gelatin based 3D scaffold presented high cell compatibility for hADSC cultivation and differentiation, which could serve as a potential implant material in clinical applications for adipose tissue reparation and regeneration.}, note = {Online available at: \url{https://doi.org/10.3233/CH-179210} (DOI). Zou, J.; Wang, W.; Neffe, A.; Xu, X.; Li, Z.; Deng, Z.; Sun, X.; Lendlein, A.: Adipogenic differentiation of human adipose derived mesenchymal stem cells in 3D architectured gelatin based hydrogels (ArcGel). Clinical Hemorheology and Microcirculation. 2017. vol. 67, no. 3-4, 297-307. DOI: 10.3233/CH-179210}} @misc{neffe_influence_of_2017, author={Neffe, A., Naolou, T., Lendlein, A.}, title={Influence of metal softness on the ring-opening polymerization of 2;5-morpholinediones and lactones}, year={2017}, howpublished = {conference lecture: Phoenix, AZ. (USA);}, note = {Neffe, A.; Naolou, T.; Lendlein, A.: Influence of metal softness on the ring-opening polymerization of 2;5-morpholinediones and lactones. MRS Spring Meeting 2017. Phoenix, AZ. (USA), 2017.}} @misc{zou_adipogenic_differentiation_2017, author={Zou, J., Wang, W., Neffe, A., Xu, X., Li, Z., Deng, Z., Sun, X., Ma, N., Lendlein, A.}, title={Adipogenic differentiation of human adipose derived mesenychmal stem cells in 3D architectured gelatin-based hydrogels (ArcGel)}, year={2017}, howpublished = {conference lecture: Greifswald (DEU);}, note = {Zou, J.; Wang, W.; Neffe, A.; Xu, X.; Li, Z.; Deng, Z.; Sun, X.; Ma, N.; Lendlein, A.: Adipogenic differentiation of human adipose derived mesenychmal stem cells in 3D architectured gelatin-based hydrogels (ArcGel). 36. Jahrestagung der Deutschen Gesellschaft für Klinische Mikrozirkulation und Hämorheologie (DGKMH). Greifswald (DEU), 2017.}} @misc{hommesschattmann_polyester_urethanes_2017, author={Hommes-Schattmann, P., Neffe, A., Zierke, M., Lendlein, A.}, title={Polyester urethanes with multiblock sequence structure and alkynyl-functionalized side chains}, year={2017}, howpublished = {conference poster: Cancun (MEX);}, abstract = {conditions.}, note = {Hommes-Schattmann, P.; Neffe, A.; Zierke, M.; Lendlein, A.: Polyester urethanes with multiblock sequence structure and alkynyl-functionalized side chains. In: XXVI International Materials Research Congress (IMRC) 2017; Symp. F4: Shape-memory and self-repairing materials. Cancun (MEX). 2017.}} @misc{neffe_clicked_gelatin_2017, author={Neffe, A., Piluso, S., Lendlein, A.}, title={Clicked gelatin hydrogels - Multifunctional networks showing enzymatic surface degradation}, year={2017}, howpublished = {conference lecture: Manchester (GBR);}, note = {Neffe, A.; Piluso, S.; Lendlein, A.: Clicked gelatin hydrogels - Multifunctional networks showing enzymatic surface degradation. Polymers for Advanced Technologies Conference 2017. Manchester (GBR), 2017.}} @misc{krgergenge_endothelialisation_of_2017, author={Krüger-Genge, A., Hauser, S., Neffe, A., Pietzsch, J., Lendlein, A., Jung, F.}, title={Endothelialisation of gelatin- based hydrogels with different elasticity and degradation time}, year={2017}, howpublished = {conference lecture: Greifswald (DEU);}, note = {Krüger-Genge, A.; Hauser, S.; Neffe, A.; Pietzsch, J.; Lendlein, A.; Jung, F.: Endothelialisation of gelatin- based hydrogels with different elasticity and degradation time. 36. Jahrestagung der Deutschen Gesellschaft für Klinische Mikrozirkulation und Hämorheologie (DGKMH). Greifswald (DEU), 2017.}} @misc{blocki_engineering_of_2017, author={Blocki, A., Löper, F., Chirico, N., Neffe, A., Jung, F., Stamm, C., Lendlein, A.}, title={Engineering of cell-laden gelatin-based microcapsules for cell delivery and immobilization in cell-based regenerative therapies}, year={2017}, howpublished = {conference lecture: Greifswald (DEU);}, note = {Blocki, A.; Löper, F.; Chirico, N.; Neffe, A.; Jung, F.; Stamm, C.; Lendlein, A.: Engineering of cell-laden gelatin-based microcapsules for cell delivery and immobilization in cell-based regenerative therapies. 36. Jahrestagung der Deutschen Gesellschaft für Klinische Mikrozirkulation und Hämorheologie. Greifswald (DEU), 2017.}} @misc{neffe_design_strategy_2016, author={Neffe, A., Federico, S., Piluso, S., Löwenberg, C., Pierce, B., Nöchel, U., Wischke, C., Lendlein, A.}, title={Design Strategy for the Elucidation of Protein-Protein-Binding Epitopes; and Application of the Derived Peptides in Biomaterials}, year={2016}, howpublished = {conference lecture: Potsdam (DEU);}, note = {Neffe, A.; Federico, S.; Piluso, S.; Löwenberg, C.; Pierce, B.; Nöchel, U.; Wischke, C.; Lendlein, A.: Design Strategy for the Elucidation of Protein-Protein-Binding Epitopes; and Application of the Derived Peptides in Biomaterials. Polydays 2016. Potsdam (DEU), 2016.}} @misc{vogt_nanocarriers_for_2016, author={Vogt, A., Wischke, C., Neffe, A.T., Ma, N., Alexiev, U., Lendlein, A.}, title={Nanocarriers for drug delivery into and through the skin - Do existing technologies match clinical challenges?}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jconrel.2016.07.027}, abstract = {The topical application of drug-loaded particles has been explored extensively aiming at a dermal, follicular or transdermal drug delivery. This review summarizes the present state of the field of polymeric nanocarriers for skin application, also covering methodologies to clinically characterize their interaction and penetration in skin in vivo. Furthermore, with a focus on a clinical perspective, a number of questions are addressed: How well are existing nanoparticle systems penetrating the skin? Which functions of new carrier concepts may meet the clinical requirements? To which extend will instrumental imaging techniques provide information on the biological functions of nanocarriers? Which issues have to be addressed for translating experimental concepts into a future clinical application?}, note = {Online available at: \url{https://doi.org/10.1016/j.jconrel.2016.07.027} (DOI). Vogt, A.; Wischke, C.; Neffe, A.; Ma, N.; Alexiev, U.; Lendlein, A.: Nanocarriers for drug delivery into and through the skin - Do existing technologies match clinical challenges?. Journal of Controlled Release. 2016. vol. 242, 3-15. DOI: 10.1016/j.jconrel.2016.07.027}} @misc{naolou_ringopening_polymerization_2016, author={Naolou, T., Neffe, A.T., Lendlein, A.}, title={Ring-Opening polymerization of morpholine-2,5-diones by iron(II) acetate and metal alkoxides}, year={2016}, howpublished = {conference poster: Berlin (D);}, note = {Naolou, T.; Neffe, A.; Lendlein, A.: Ring-Opening polymerization of morpholine-2,5-diones by iron(II) acetate and metal alkoxides. In: International Conference on Dermal Drug Delivers by Nanocarrierrs, SFB Konferenz. Berlin (D). 2016.}} @misc{zhang_compositiondependent_skin_2016, author={Zhang, N., Said, A., Wischke, C., Kral, V., Brodwolf, R., Boreham, A., Gerecke, C., Li, W., Neffe, A.T., Kleuser, B., Alexiev, U., Lendlein, A., Schaefer-Korting, M.}, title={Composition-dependent skin penetration and toxicity of a series of poly[acrylonitrile-co-(N-vinyl pyrrolidone)] nanoparticles}, year={2016}, howpublished = {conference poster: Berlin (D);}, note = {Zhang, N.; Said, A.; Wischke, C.; Kral, V.; Brodwolf, R.; Boreham, A.; Gerecke, C.; Li, W.; Neffe, A.; Kleuser, B.; Alexiev, U.; Lendlein, A.; Schaefer-Korting, M.: Composition-dependent skin penetration and toxicity of a series of poly[acrylonitrile-co-(N-vinyl pyrrolidone)] nanoparticles. In: International Conference on Dermal Drug Delivers by Nanocarrierrs, SFB Konferenz. Berlin (D). 2016.}} @misc{schulz_monolayer_formation_2016, author={Schulz, C., Vukicevic, R., Krueger-Genge, A., Neffe, A.T., Lendlein, A., Jung, F.}, title={Monolayer formation and shear-resistance of human umbilical vein endothelial cells on gelatin-based hydrogels with tailorable elsticity and degradability}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-168007}, abstract = {The formation of a functionally-confluent and shear-resistant endothelial cell monolayer on cardiovascular implants is a promising strategy to prevent thrombogenic processes after implantation. On the basis of existing studies with arterial endothelial cells adhering after two hours on gelatin-based hydrogels in marked higher numbers compared to tissue culture plates, we hypothesize that also venous endothelial cells (HUVEC) should be able to adhere and form an endothelial monolayer on these hydrogels after days. Furthermore, variation of the hydrogel composition, which slightly influences the materials elasticity and even more the degradation behaviour, should have no considerable effect on HUVEC. Therefore, the monolayer formation and shear resistance of HUVEC were explored on two gelatin-based hydrogels differing in their elasticity (Young’s moduli between 35 and 55 kPa) in comparison to a positive control (HUVEC on glass cover slips) and a negative control (HUVEC on glass cover slips activated with interleukin-1β) after 9 days of culturing. HUVEC density after 9 days of culturing under static conditions was lower on the hydrogels compared to both controls (p < 0.05 each). On G10_LNCO8 slightly more EC adhered than on G10_LNCO5. Staining of the actin cytoskeleton and VE-cadherin revealed a pronounced cell-substrate interaction while the cell-cell interaction was comparable to the controls (HUVEC on glass). The secretion of vasoactive and inflammatory mediators did not differ between the hydrogels and the controls. Adherent HUVEC seeded on the hydrogels were able to resist physiological shear forces and the release of cyto- and chemokines in response to the shear forces did not differ from controls (HUVEC on glass). Therefore, both gelatin-based hydrogels are a suitable substrate for EC and a promising candidate for cardiovascular applications.}, note = {Online available at: \url{https://doi.org/10.3233/CH-168007} (DOI). Schulz, C.; Vukicevic, R.; Krueger-Genge, A.; Neffe, A.; Lendlein, A.; Jung, F.: Monolayer formation and shear-resistance of human umbilical vein endothelial cells on gelatin-based hydrogels with tailorable elsticity and degradability. Clinical Hemorheology and Microcirculation. 2016. vol. 64, no. 4, 699-710. DOI: 10.3233/CH-168007}} @misc{tondera_gelatinbased_hydrogel_2016, author={Tondera, C., Hauser, S., Krueger-Genge, A., Jung, F., Neffe, A.T., Lendlein, A., Klopfleisch, R., Steinbach, J., Neuber, C., Pietzsch, J.}, title={Gelatin-based Hydrogel Degradation and Tissue Interaction in vivo: Insights from Multimodal Preclinical Imaging in Immunocompetent Nude Mice}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.7150/thno.16614}, abstract = {Hydrogels based on gelatin have evolved as promising multifunctional biomaterials. Gelatin is crosslinked with lysine diisocyanate ethyl ester (LDI) and the molar ratio of gelatin and LDI in the starting material mixture determines elastic properties of the resulting hydrogel. In order to investigate the clinical potential of these biopolymers, hydrogels with different ratios of gelatin and diisocyanate (3-fold (G10_LNCO3) and 8-fold (G10_LNCO8) molar excess of isocyanate groups) were subcutaneously implanted in mice (uni- or bilateral implantation). Degradation and biomaterial-tissue-interaction were investigated in vivo (MRI, optical imaging, PET) and ex vivo (autoradiography, histology, serum analysis). Multimodal imaging revealed that the number of covalent net points correlates well with degradation time, which allows for targeted modification of hydrogels based on properties of the tissue to be replaced. Importantly, the degradation time was also dependent on the number of implants per animal. Despite local mechanisms of tissue remodeling no adverse tissue responses could be observed neither locally nor systemically. Finally, this preclinical investigation in immunocompetent mice clearly demonstrated a complete restoration of the original healthy tissue.}, note = {Online available at: \url{https://doi.org/10.7150/thno.16614} (DOI). Tondera, C.; Hauser, S.; Krueger-Genge, A.; Jung, F.; Neffe, A.; Lendlein, A.; Klopfleisch, R.; Steinbach, J.; Neuber, C.; Pietzsch, J.: Gelatin-based Hydrogel Degradation and Tissue Interaction in vivo: Insights from Multimodal Preclinical Imaging in Immunocompetent Nude Mice. Theranostics. 2016. vol. 6, no. 12, 2114-2128. DOI: 10.7150/thno.16614}} @misc{tzoneva_angiogenic_potential_2016, author={Tzoneva, R., Uzunova, V., Apostolova, S., Krueger-Genge, A., Neffe, A.T., Jung, F., Lendlein, A.}, title={Angiogenic potential of endothelial and tumor cells seeded on gelatin–based hydrogels in response to electrical stimulations}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-168040}, abstract = {Angiogenesis is one of the key processes during development, wound healing and tumor formation. Prerequisite for its existence is the presence of endogenous electrical fields (EFs) generated by active ion transport across polarized epithelia and endothelia, and appearance of the transcellular potentials. During angiogenesis cellular factor as endothelial growth factor (VEGF), synthesis of adhesive proteins and membrane metalloproteinases (MMPs) govern the angiogenic response to different external stimuli as biomaterials interactions and/or exogenous EF. Gelatin-based hydrogels with elasticities comparable to human tissues have shown to influence cell behavior as well as cell attachment, protein synthesis, VEGF and MMP’s production after the application of EF. Gelatin-based matrices with 3 (G10_LNCO3), 5 (G10_LNCO5), and 8 (G10_LNCO8) fold excess of isocyanate groups per mol of amine groups present in gelatin were used. Human umbilical endothelial cells (HUVEC) (Lonza Basel, Switzerland) and highly invasive breast cancer MDA-MB-231 cells (ATCC®HTB-26TM) were used. For an estimation of the amount of VEGF released from cells a commercially available VEGF ELISA (Thermo Fisher Scientific, Germany) kit was used. Fibronectin (FN) enzyme immunoassay (EIA) was used to analyze the secreted amount of FN by cells seeded on the materials. Secreted MMPs were analyzed by zymography. Gelatin-based hydrogels attracted HUVEC adhesion and diminished the adhesion of MDA-MB-231 cells. The applied direct current (DC) EF induced an almost 5–fold increase in VEGF production by HUVEC seeded on gelatin-based hydrogels, while in contrast, the applied EF decreased the production of VEGF by cancer cells. FN synthesis was elevated in HUVEC cells seeded on gelatin-based materials in comparison to FN synthesis by cancer cells. HUVEC seeded on gelatin hydrogels showed an expression mainly of MMP-2. The application of EF increased the production of MMP-2 in HUVEC seeded on gelatin materials. In contrast, for MDA-MB-231 the production of MMPs on gelatin materials was lower compared to control materials. With the application of EF the levels of MMP-9 decreased but MMP-2 expression raised significantly for gelatin materials. Overall, the results showed that studied gelatin materials suppressed attachment of cancerous cells, as well as suppressed their angiogenic potential revealed by decreased VEGF and MMP production. Thus, this study approved gelatin-based hydrogels with proper elasticity characteristics and different degradation behavior as useful matrices for use in vascular tissue regeneration or in restriction of tumor growth after tumor resection.}, note = {Online available at: \url{https://doi.org/10.3233/CH-168040} (DOI). Tzoneva, R.; Uzunova, V.; Apostolova, S.; Krueger-Genge, A.; Neffe, A.; Jung, F.; Lendlein, A.: Angiogenic potential of endothelial and tumor cells seeded on gelatin–based hydrogels in response to electrical stimulations. Clinical Hemorheology and Microcirculation. 2016. vol. 64, no. 4, 941-949. DOI: 10.3233/CH-168040}} @misc{tzoneva_angiogenic_potential_2016, author={Tzoneva, R., Uzunova, V., Apostolova, S., Krueger-Genge, A., Neffe, A.T., Jung, F., Lendlein, A.}, title={Angiogenic potential of endothelial and tumor cells seeded on gelatin–based hydrogels in response to electrical stimulations}, year={2016}, howpublished = {conference lecture: Lissabon (P);}, note = {Tzoneva, R.; Uzunova, V.; Apostolova, S.; Krueger-Genge, A.; Neffe, A.; Jung, F.; Lendlein, A.: Angiogenic potential of endothelial and tumor cells seeded on gelatin–based hydrogels in response to electrical stimulations. 18th Conference of the European Society for Clinical Hemorheology and Microcirculation, ESCHM 2016. Lissabon (P), 2016.}} @misc{tondera_gelatinbased_hydrogels_2016, author={Tondera, C., Ullm, S., Krüger-Genge, A., Jung, F., Gebauer, T., Neffe, A., Lendlein, A., Steinbach, J., Pietzsch, J.}, title={Gelatin-based hydrogels as versatile tools for tissue engineering: insights from multimodal imaging in vivo and ex vivo}, year={2016}, howpublished = {conference lecture: Montréal (CAN);}, note = {Tondera, C.; Ullm, S.; Krüger-Genge, A.; Jung, F.; Gebauer, T.; Neffe, A.; Lendlein, A.; Steinbach, J.; Pietzsch, J.: Gelatin-based hydrogels as versatile tools for tissue engineering: insights from multimodal imaging in vivo and ex vivo. World Biomaterials Congress (WBC 2016). Montréal (CAN), 2016.}} @misc{neffe_secondary_structure_2016, author={Neffe, A.T., Federico, S., Lendlein, A.}, title={Secondary Structure of Decorin-Derived Peptides in Solution}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.1557/adv.2016.266}, abstract = {Decorin is a small leucine-rich repeat proteoglycan supporting collagen fibril formation by controlling the rate of collagen fibrillogenesis and fibril dimensions. Peptides derived from the inner surface of decorin have been shown to bind to collagen, while peptides derived from the outer surface do not display such binding affinity. As typical secondary structural elements such as β-sheets and α-helical regions were found in the decorin X-ray crystal structure, here it was investigated by Circular Dichroism (CD) spectroscopy in solution, whether the same structural elements can be found in the derived peptides. Here it is shown that the peptide derived from decorin’s outer surface has the propensity to adopt helical conformation, as it was found in the crystal structure. The results were more pronounced in 80 vol% TFE solution, which led to an increase in the number as well as the length of helices. In contrast, peptides derived from the inner surface had a higher tendency to adopt β-sheet conformation, also in TFE, which corresponds to the conformation of the original sequence in the crystal structure of decorin. This suggests that the peptides derived from decorin adopt the structures present in the native protein.}, note = {Online available at: \url{https://doi.org/10.1557/adv.2016.266} (DOI). Neffe, A.; Federico, S.; Lendlein, A.: Secondary Structure of Decorin-Derived Peptides in Solution. MRS Advances. 2016. vol. 1, no. 27, 1965-1970. DOI: 10.1557/adv.2016.266}} @misc{vukicevic_mechanical_properties_2016, author={Vukicevic, R., Neffe, A.T., Gebauer, T., Frank, O., Schossig, M., Lendlein, A.}, title={Mechanical Properties of Architectured Gelatin-Based Hydrogels on Different Hierarchical Levels}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.1557/adv.2016.416}, abstract = {Preparation of three-dimensionally architectured porous biomaterials can be achieved in a one-step process by stabilizing gelatin with L-lysine diisocyanate ethyl ester (LDI) in water. The reaction of gelatin with LDI in presence of water leads to the formation of oligourea bridges between gelatin molecules and oligourea chains grafted on gelatin. The number and the length of the bridges, as well as of the grafted chains strongly depend on the concentration of the LDI used for the stabilization, and this has huge influence on the mechanical properties of the material on different hierarchical levels. Higher LDI concentrations yield materials with increased deformation resistance in tensile tests due to the higher number of covalent and physical netpoints in the material. However, mechanical properties determined on the micro-level by AFM indentation showed the opposite trend, i.e. a decrease of Young’s modulus with increasing LDI content. This was interpreted by a decreasing number of shorter oligourea bridges between gelatin chains with decreasing LDI content.}, note = {Online available at: \url{https://doi.org/10.1557/adv.2016.416} (DOI). Vukicevic, R.; Neffe, A.; Gebauer, T.; Frank, O.; Schossig, M.; Lendlein, A.: Mechanical Properties of Architectured Gelatin-Based Hydrogels on Different Hierarchical Levels. MRS Advances. 2016. vol. 1, no. 27, 1995-2001. DOI: 10.1557/adv.2016.416}} @misc{neffe_editorial_advanced_2016, author={Neffe, A.T., Grijpma, D.W., Lendlein, A.}, title={Editorial: Advanced Functional Polymers for Medicine}, year={2016}, howpublished = {Other: editorial}, doi = {https://doi.org/10.1002/mabi.201600419}, abstract = {No abstract}, note = {Online available at: \url{https://doi.org/10.1002/mabi.201600419} (DOI). Neffe, A.; Grijpma, D.; Lendlein, A.: Editorial: Advanced Functional Polymers for Medicine. Macromolecular Bioscience. 2016. vol. 16, no. 12, 1743-1744. DOI: 10.1002/mabi.201600419}} @misc{naolou_influence_of_2016, author={Naolou, T., Lendlein, A., Neffe A.T.}, title={Influence of metal softness on the metal-organic catalyzed polymerization of morpholin-2,5-diones to oligodepsipeptides}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.eurpolymj.2016.10.011}, abstract = {Synthetic access to oligodepsipeptides (ODP), polymers with high potential in biomedicine, is given by the ring-opening polymerization (ROP) of morpholine-2,5-diones (MDs). Classically, the ROP of MDs is mostly conducted by coordination-insertion polymerization using metal-organics as a catalyst e.g. tin(II) di(2-ethyl hexanoate) (Sn(Oct)2). This ROP has been shown to be significantly more difficult to conduct than the corresponding ROP of dilactide, which was related to different electronic properties of the monomers and potential steric crowding. Here, we investigated the ROP of 3-(S)-sec-butylmorpholine-2,5-dione (BMD) by varying the catalyst’s hardness, comparing Sn(Oct)2 with the ethoxides of indium, magnesium, aluminum and iron(III), as well as with iron(II) acetate. The ROP of BMD with Sn(Oct)2 in bulk at 135 °C for 24 h gave ODP with a number-average molecular weight (Mn) = 4.5 kDa. Mg(OEt)2 gave the best results among the other investigated metal ethoxides with ODP of Mn = 4 kDa and a conversion ratio of 57 mol%. On the other hand, high polymerization temperature was needed (160 °C) in the case of In(OEt)3, which resulted in partial degradation, while Al(OEt)3 and Fe(OEt)3 did not result in polymerization. Very effective for the ROP of the studied MD proofed to be Fe(OAc)2, giving OBMD with a Mn = 5.8 kDa, a polydispersity of 1.1, a conversion ratio of 86 mol%, and no racemization. This catalyst likewise performed well in the polymerization of Ser- and Tyr-based MDs. Fe(II) is softer than Sn(II) and may support the ROP by promoting the alkoxide transfer step of the polymerization, while suppressing the formation of unreactive coordination complexes. In contrast, the metal alkoxides investigated were harder than Fe(II) or Sn(II), but had low steric demand. The results suggest that the hardness of the central atom is the key property in the polymerization, while steric considerations are of lower importance. In addition, a synthesis of MDs with protected side chains in improved yields was introduced. This was achieved by in situ formation of an alkyl iodide that is very effective in the ring closing reaction.}, note = {Online available at: \url{https://doi.org/10.1016/j.eurpolymj.2016.10.011} (DOI). Naolou, T.; Lendlein, A.; Neffe A.T.: Influence of metal softness on the metal-organic catalyzed polymerization of morpholin-2,5-diones to oligodepsipeptides. European Polymer Journal. 2016. vol. 85, 139-149. DOI: 10.1016/j.eurpolymj.2016.10.011}} @misc{schulz_monolayer_formation_2016, author={Schulz, C., Vukicevic, R., Krueger-Genge, A., Neffe, A.T., Lendlein, A., Jung, F.}, title={Monolayer formation and shear-resistance of human umbilical vein endothelial cells on gelatin-based hydrogels with tailorable elsticity and degradability}, year={2016}, howpublished = {conference lecture: Lissabon (P);}, note = {Schulz, C.; Vukicevic, R.; Krueger-Genge, A.; Neffe, A.; Lendlein, A.; Jung, F.: Monolayer formation and shear-resistance of human umbilical vein endothelial cells on gelatin-based hydrogels with tailorable elsticity and degradability. 18th European Conference for Clinical Hemorheology and Microcirculation, ESCHM 2016. Lissabon (P), 2016.}} @misc{schulz_cell_layer_2016, author={Schulz, C., Vukicevic, R., Krueger-Genge, A., Neffe, A.T., Lendlein, A., Jung, F.}, title={Cell layer formation and shear-resistance of human endothelial cells on gelatin-based hydrogels with tailorable elasticity}, year={2016}, howpublished = {conference lecture: Lissabon (P);}, note = {Schulz, C.; Vukicevic, R.; Krueger-Genge, A.; Neffe, A.; Lendlein, A.; Jung, F.: Cell layer formation and shear-resistance of human endothelial cells on gelatin-based hydrogels with tailorable elasticity. 18th Conference of the European Society for Clinical Hemorheology and Microcirculation, ESCHM 2016. Lissabon (P), 2016.}} @misc{federico_supramolecular_hydrogel_2016, author={Federico, S., Noechel, U., Loewenberg, C., Lendlein, A., Neffe, A.T.}, title={Supramolecular hydrogel networks formed by molecular recognition of collagen and a peptide grafted to hyaluronic acid}, year={2016}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.actbio.2016.04.018}, abstract = {The extracellular matrix (ECM) is a nano-structured, highly complex hydrogel, in which the macromolecules are organized primarily by non-covalent interactions. Here, in a biomimetic approach, the decorin-derived collagen-binding peptide LSELRLHNN was grafted to hyaluronic acid (HA) in order to enable the formation of a supramolecular hydrogel network together with collagen. The storage modulus of a mixture of collagen and HA was increased by more than one order of magnitude (G′ = 157 Pa) in the presence of the HA-grafted peptide compared to a mixture of collagen and HA (G′ = 6 Pa). The collagen fibril diameter was decreased, as quantified using electron microscopy, in the presence of the HA-grafted peptide. Here, the peptide mimicked the function of decorin by spatially organizing collagen. The advantage of this approach is that the non-covalent crosslinks between collagen molecules and the HA chains created by the peptide form a reversible and dynamic hydrogel, which could be employed for a diverse range of applications in regenerative medicine.}, note = {Online available at: \url{https://doi.org/10.1016/j.actbio.2016.04.018} (DOI). Federico, S.; Noechel, U.; Loewenberg, C.; Lendlein, A.; Neffe, A.: Supramolecular hydrogel networks formed by molecular recognition of collagen and a peptide grafted to hyaluronic acid. Acta Biomaterialia. 2016. vol. 38, 1-10. DOI: 10.1016/j.actbio.2016.04.018}} @misc{hudson_classifying_calpain_2016, author={Hudson, I.L., Leemaqz, S.Y., Neffe, A.T., Abell, A.D.}, title={Classifying calpain inhibitors for the treatment of cataracts: a Self Organising Map (SOM) ANN/KM approach in drug discovery}, year={2016}, howpublished = {book part}, doi = {https://doi.org/10.1007/978-3-319-28495-8_9}, abstract = {number of FNs by 64 % and FPs by 26 %, compared to the glide score alone. FPs were shown to be mostly esters and amides plus alcohols and non-classical, and FNs mainly aldehydes and ketones, masked aldehydes and ketones and Michael.}, note = {Online available at: \url{https://doi.org/10.1007/978-3-319-28495-8_9} (DOI). Hudson, I.; Leemaqz, S.; Neffe, A.; Abell, A.: Classifying calpain inhibitors for the treatment of cataracts: a Self Organising Map (SOM) ANN/KM approach in drug discovery. In: Shanmuganathan, S. (Ed.): Artificial Neural Network Modelling - Studies in Computational Intelligence. Springer. 2016. 161-212. DOI: 10.1007/978-3-319-28495-8_9}} @misc{lendlein_functional_polymers_2016, author={Lendlein, A., Neffe, A.T., Ma, N., Behl, M., Wischke, C.}, title={Functional Polymers and Carriers Systems}, year={2016}, howpublished = {conference lecture: Berlin (D);}, note = {Lendlein, A.; Neffe, A.; Ma, N.; Behl, M.; Wischke, C.: Functional Polymers and Carriers Systems. International Conference on Dermal Drug Delivers by Nanocarrierrs, SFB Konferenz. Berlin (D), 2016.}} @misc{brunacci_evaluation_of_2016, author={Brunacci, N., Naolou, T., Neffe, A.T., Wischke, C., Lendlein, A.}, title={Evaluation of surfactants for the formation of sub-micron depsipeptide particles}, year={2016}, howpublished = {conference poster: Berlin (D);}, note = {Brunacci, N.; Naolou, T.; Neffe, A.; Wischke, C.; Lendlein, A.: Evaluation of surfactants for the formation of sub-micron depsipeptide particles. In: International Conference on Dermal Drug Delivers by Nanocarrierrs, SFB Konferenz. Berlin (D). 2016.}} @misc{neffe_sequential_alkyneazide_2016, author={Neffe, A.T., Piluso, S., Vukicevic, R., Noechel, U., Braune, S., Lendlein, A.}, title={Sequential Alkyne-Azide Cycloadditions for Functionalized Gelatin Hydrogel Formation}, year={2016}, howpublished = {conference poster: Twente (NL);}, note = {Neffe, A.; Piluso, S.; Vukicevic, R.; Noechel, U.; Braune, S.; Lendlein, A.: Sequential Alkyne-Azide Cycloadditions for Functionalized Gelatin Hydrogel Formation. In: Advanced Functional Polymers for Medicine, AFPM 2016. Twente (NL). 2016.}} @misc{hommesschattmann_electrospun_ppdo_2016, author={Hommes-Schattmann, P.J., Neffe, A.T., Ahmad, B., Williams, G.R., Vanneaux, V., Menasche, P., Kalfa, D., Lendlein, A.}, title={Electrospun PPDO- Patches With Incorporated RGD Constructs}, year={2016}, howpublished = {conference poster: Twente (NL);}, note = {Hommes-Schattmann, P.; Neffe, A.; Ahmad, B.; Williams, G.; Vanneaux, V.; Menasche, P.; Kalfa, D.; Lendlein, A.: Electrospun PPDO- Patches With Incorporated RGD Constructs. In: Advanced Functional Polymers for Medicine, AFPM 2016. Twente (NL). 2016.}} @misc{blocki_response_of_2016, author={Blocki, A., Loewenberg, C., Jiang, Y., Kratz, K., Neffe, A.T., Jung, F., Lendlein, A.}, title={Response of encapsulated cells to a gelatin matrix with varied bulk and microenvironmental elastic properties}, year={2016}, howpublished = {conference poster: Berlin (D);}, note = {Blocki, A.; Loewenberg, C.; Jiang, Y.; Kratz, K.; Neffe, A.; Jung, F.; Lendlein, A.: Response of encapsulated cells to a gelatin matrix with varied bulk and microenvironmental elastic properties. In: RegMed Forum - 10 Jahre BCRT. Berlin (D). 2016.}} @misc{blocki_mechanobiological_response_2016, author={Blocki, A., Loewenberg, C., Neffe, A.T., Jung, F., Lendlein, A.}, title={Mechanobiological response of encapsulated cells to a gelatin matrix with varied crosslinking density}, year={2016}, howpublished = {conference poster: Twente (NL);}, note = {Blocki, A.; Loewenberg, C.; Neffe, A.; Jung, F.; Lendlein, A.: Mechanobiological response of encapsulated cells to a gelatin matrix with varied crosslinking density. In: Advanced Functional Polymers for Medicine, AFPM 2016. Twente (NL). 2016.}} @misc{lendlein_functional_polymers_2016, author={Lendlein, A., Neffe, A.T., Ma, N., Behl, M., Wischke, C.}, title={Functional Polymers and Carriers Systems}, year={2016}, howpublished = {conference lecture: Berlin (D);}, note = {Lendlein, A.; Neffe, A.; Ma, N.; Behl, M.; Wischke, C.: Functional Polymers and Carriers Systems. Multifunctional Biomaterials for Medicine, HVI Symposium 2016. Berlin (D), 2016.}} @misc{brunacci_comparison_of_2016, author={Brunacci, N., Naolou, T., Wischke, C., Neumann, F., Ma, N., Neffe, A.T., Lendlein, A.}, title={Comparison of particulate carriers for dexamethasone with oligodepsipeptide or OLGA as matrix material}, year={2016}, howpublished = {conference poster: Twente (NL);}, note = {Brunacci, N.; Naolou, T.; Wischke, C.; Neumann, F.; Ma, N.; Neffe, A.; Lendlein, A.: Comparison of particulate carriers for dexamethasone with oligodepsipeptide or OLGA as matrix material. In: Advanced Functional Polymers for Medicine, AFPM 2016. Twente (NL). 2016.}} @misc{behl_design_principles_2016, author={Behl, M., Neffe, A., Kratz, K., Ma, N., Lendlein, A.}, title={Design Principles of Multifunctional Materials Interacting With Cells}, year={2016}, howpublished = {conference lecture (invited): Stuttgart (DEU);}, note = {Behl, M.; Neffe, A.; Kratz, K.; Ma, N.; Lendlein, A.: Design Principles of Multifunctional Materials Interacting With Cells. Medtec Europe. Stuttgart (DEU), 2016.}} @misc{schulz_monolayer_formation_2016, author={Schulz, C., Krueger-Genge, A., Vukicevic, R., Neffe, A.T., Lendlein, A., Jung, F.}, title={Monolayer formation and shear-resistance of human vein endothelial cells on gelatin-based hydrogels with tailorable elasticity and degradability}, year={2016}, howpublished = {conference lecture: Lissabon (P);}, note = {Schulz, C.; Krueger-Genge, A.; Vukicevic, R.; Neffe, A.; Lendlein, A.; Jung, F.: Monolayer formation and shear-resistance of human vein endothelial cells on gelatin-based hydrogels with tailorable elasticity and degradability. 18th Conference of the European Society for Clinical Hemorheology and Microcirculation, ESCHM 2016. Lissabon (P), 2016.}} @misc{brunacci_depsipeptide_submicron_2016, author={Brunacci, N., Naolou, T., Wischke, C., Neffe, A., Lendlein, A.}, title={Depsipeptide submicron particles of different sizes as drug delivery systems}, year={2016}, howpublished = {conference poster: Potsdam (DEU);}, note = {Brunacci, N.; Naolou, T.; Wischke, C.; Neffe, A.; Lendlein, A.: Depsipeptide submicron particles of different sizes as drug delivery systems. In: Polydays 2016. Potsdam (DEU). 2016.}} @misc{neffe_one_step_2016, author={Neffe, A., Pierce, B., Tronci, G., Ma, N., Pittermann, E., Gebauer, T., Frank, O., Schossig, M., Xu, X., Willie, B., Forner, M., Ellinghaus, A., Lienau, J., Duda, G., Lendlein, A.}, title={One step creation of multifunctional 3D architectured hydrogels inducing bone regeneration}, year={2016}, howpublished = {conference lecture: Berlin (DEU);}, note = {Neffe, A.; Pierce, B.; Tronci, G.; Ma, N.; Pittermann, E.; Gebauer, T.; Frank, O.; Schossig, M.; Xu, X.; Willie, B.; Forner, M.; Ellinghaus, A.; Lienau, J.; Duda, G.; Lendlein, A.: One step creation of multifunctional 3D architectured hydrogels inducing bone regeneration. RegMed Forum & 10 Years BCRT. Berlin (DEU), 2016.}} @misc{julichgruner_synthesis_and_2015, author={Julich-Gruner, K.K., Roch, T., Ma, N., Neffe, A.T., Lendlein, A.}, title={Synthesis and characterization of star-shaped oligo(ethylene glycol) with tyrosine derived moieties under variation of their molecular weight}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-151938}, abstract = {Conclusively, our data suggested that the sOEG solutions have surface active properties without inducing unwanted cellular responses, which is required e.g. in pharmaceutical applications to solubilize hydophobic substances.}, note = {Online available at: \url{https://doi.org/10.3233/CH-151938} (DOI). Julich-Gruner, K.; Roch, T.; Ma, N.; Neffe, A.; Lendlein, A.: Synthesis and characterization of star-shaped oligo(ethylene glycol) with tyrosine derived moieties under variation of their molecular weight. Clinical Hemorheology and Microcirculation. 2015. vol. 60, no. 1, 13-23. DOI: 10.3233/CH-151938}} @misc{roch_immunocompatibility_of_2015, author={Roch, T., Julich-Gruner, K.K., Neffe, A.T., Ma, N., Leindlein, A.}, title={Immuno-compatibility of desaminotyrosine and desaminotyrosyl tyrosine functionalized star-shaped oligo(ethylene glycol)s with different molecular weights}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2015.327}, abstract = {Polymer-based therapeutic strategies require biomaterials with properties and functions tailored to the demands of specific applications leading to an increasing number of newly designed polymers. For the evaluation of those new materials, comprehensive biocompatibility studies including cyto-, tissue-, and immunocompatibility are essential. Recently, it could be demonstrated that star-shaped amino oligo(ethylene glycol)s (sOEG) with a number average molecular weight of 5 kDa and functionalized with the phenol-derived moieties desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT) behave in aqueous solution like surfactants without inducing a substantial cytotoxicity, which may qualify them as solubilizer for hydrophobic drugs in aqueous solution. However, for biomedical applications the polymer solutions need to be free of immunogenic contaminations, which could result from inadequate laboratory environment or contaminated starting material. Furthermore, the materials should not induce uncontrolled or undesired immunological effects arising from material intrinsic properties. Therefore, a comprehensive immunological evaluation as perquisite for application of each biomaterial batch is required. This study investigated the immunological properties of sOEG-DAT(T) solutions, which were prepared using sOEG with number average molecular weights of 5 kDa, 10 kDa, and 20 kDa allowing analyzing the influence of the sOEG chain lengths on innate immune mechanisms. A macrophage-based assay was used to first demonstrate that all DAT(T)-sOEG solutions are free of endotoxins and other microbial contaminations such as fungal products. In the next step, the capacity of the different DAT(T)-functionalized sOEG solutions to induce cytokine secretion and generation of reactive oxygen species (ROS) was investigated using whole human blood. It was observed that low levels of the pro-inflammatory cytokines interleukin(IL)-1β and IL-6 were detected for all sOEG solutions but only when used at concentrations above 250 µg·mL-1. Furthermore, only the 20 kDa sOEG-DAT induced low amounts of ROS-producing monocytes. Conclusively, the data indicate that the materials were not contaminated with microbial products and do not induce substantial immunological adverse effects in vitro, which is a prerequisite for future biological applications.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2015.327} (DOI). Roch, T.; Julich-Gruner, K.; Neffe, A.; Ma, N.; Leindlein, A.: Immuno-compatibility of desaminotyrosine and desaminotyrosyl tyrosine functionalized star-shaped oligo(ethylene glycol)s with different molecular weights. MRS Online Proceedings Library. 2015. vol. 1718, DOI: 10.1557/opl.2015.327}} @misc{lohmann_untersuchung_der_2015, author={Lohmann, P., Willuweit, A., Geisler, S., Gebauer, T., Neffe, A., Lendlein, A., Shah, N., Langen, K.}, title={Untersuchung der Knochenregeneration mittels 18F-Fluorid und 18F-FDG mittels Inveon PET/CT im kritischen Knochendefektmodell bei der Ratte}, year={2015}, howpublished = {conference poster: Hannover (DEU);}, note = {Lohmann, P.; Willuweit, A.; Geisler, S.; Gebauer, T.; Neffe, A.; Lendlein, A.; Shah, N.; Langen, K.: Untersuchung der Knochenregeneration mittels 18F-Fluorid und 18F-FDG mittels Inveon PET/CT im kritischen Knochendefektmodell bei der Ratte. In: Konferenz der Deutschen Gesellschaft für Nuklearmedizin (DGN). Hannover (DEU). 2015.}} @misc{neffe_strategy_for_2015, author={Neffe, A., Federico, S., Lendlein, A.}, title={Strategy for the Computer-assisted Design of Collagen Binding Peptides and their Application in Biomaterial Design}, year={2015}, howpublished = {conference lecture (invited): Galway (IRL);}, note = {Neffe, A.; Federico, S.; Lendlein, A.: Strategy for the Computer-assisted Design of Collagen Binding Peptides and their Application in Biomaterial Design. Advanced Functional Polymers for Medicine (AFPM). Galway (IRL), 2015.}} @misc{federico_design_von_2015, author={Federico, S., Pierce, B.F., Piluso, S., Lendlein, A., Neffe, A.T.}, title={Design von Decorin-basierten Peptiden, die an Kollagen I binden, und ihr Potenzial als Adhaesionssequenzen in Biomaterialien}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1002/ange.201505227}, abstract = {Das Nachbilden der Bindungsepitope von Protein-Protein-Wechselwirkungen mithilfe kleiner Peptide ist wichtig bei der Entwicklung modularer biomimetischer Systeme. Hier beschreiben wir eine Strategie zum Design solcher bioaktiver Peptide, ohne dass Strukturdaten des Proteinkomplexes benötigt werden, und weisen den Effekt der Inkorporierung solcher Adhäsionssequenzen in komplexen Biomaterialsystemen nach. Dazu wurde die hochrepetitive Struktur von Decorin analysiert. Für dessen innere sowie äußere Oberfläche wurden repräsentative Peptide identifiziert und synthetisiert. Nur Peptide auf Basis der inneren Oberfläche binden an Kollagen. Das Peptid mit der höchsten Bindungsaffinität für Kollagen I führte zu einer geringeren Diffusionsgeschwindigkeit eines gekuppelten Farbstoffs in einem Kollagengel. Dimere des Peptids ermöglichten eine physikalische Vernetzung von Kollagen, wodurch der Speichermodul eines Gels stark erhöht werden konnte. Dies belegt das Potenzial der Peptide für das Design von Biomaterialien für die regenerative Medizin.}, note = {Online available at: \url{https://doi.org/10.1002/ange.201505227} (DOI). Federico, S.; Pierce, B.; Piluso, S.; Lendlein, A.; Neffe, A.: Design von Decorin-basierten Peptiden, die an Kollagen I binden, und ihr Potenzial als Adhaesionssequenzen in Biomaterialien. Angewandte Chemie. 2015. vol. 127, no. 37, 11131-11135. DOI: 10.1002/ange.201505227}} @misc{loewenberg_influence_of_2015, author={Loewenberg, C., Julich-Gruner, K.K., Neffe, A.T.Lendlein, A.}, title={Influence of glycidylmethacrylate functional groups attached to gelatin on the formation and properties of hydrogels}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2015.491}, abstract = {Gelatin functionalized with glycidyl methacrylate (GMA) has been shown to allow crosslinking by photopolymerization and metathesis reaction. However, side chain functionalization of gelatin might reduce triple helicalization, which influences mechanical properties of gelatin-based polymer networks. Here, the influence of glycidylmethycrylation of gelatin on the chain organization, swelling, and mechanical properties is investigated by comparing among each other physical gels prepared from GMA-gelatin solutions of different concentrations (5-20 wt.-%) by drying and rehydration. An increase of GMA-gelatin concentration from 5 wt.-% to 20 wt.-% led to an increased density of produced gelatin films and a decreasing water uptake of the films from 1160 wt.-% to 730 wt.-%, while the storage modulus was increasing about one order of magnitude from 440 Pa to 4090 Pa. The relative single and triple helix content was not influenced by the variation of polymer concentration.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2015.491} (DOI). Loewenberg, C.; Julich-Gruner, K.; Neffe, A.: Influence of glycidylmethacrylate functional groups attached to gelatin on the formation and properties of hydrogels. MRS Online Proceedings Library. 2015. vol. 1718, 76-81. DOI: 10.1557/opl.2015.491}} @misc{vukicevic_conditional_ultrasound_2015, author={Vukicevic, R., Neffe, A.T., Luetzow, K., Pierce, B.F., Lendlein, A.}, title={Conditional Ultrasound Sensitivity of Poly[(N-isopropylacrylamide)-co-(vinyl imidazole)] Microgels for Controlled Lipase Release}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1002/marc.201500311}, abstract = {Triggering the release of cargo from a polymer network by ultrasonication as an external, noninvasive stimulus can be an interesting concept for on-demand release. Here, it is shown that, in pH- and thermosensitive microgels, the ultrasound sensitivity of the polymer network depends on the external conditions. Crosslinked poly[(N-isopropylacrylamide)-co-(vinyl imidazole)] microgels showed a volume phase transition temperature (VPTT) of 25–50 °C, which increases with decreasing pH. Above the VPTT the polymer chains are collapsed, while below VPTT they are extended. Only in the case of maximum observed swelling, where the polymer chains are expanded, the microgels are mechanically fragmented through ultrasonication. In contrast, when the polymer chains are partially collapsed it is not possible to manipulate the microgels by ultrasound. Additionally, the ultrasound-induced on-demand release of wheat germ lipase from the microgels could be demonstrated successfully. The principle of conditional ultrasound sensitivity is likely to be general and can be used for selection of matrix–cargo combinations.}, note = {Online available at: \url{https://doi.org/10.1002/marc.201500311} (DOI). Vukicevic, R.; Neffe, A.; Luetzow, K.; Pierce, B.; Lendlein, A.: Conditional Ultrasound Sensitivity of Poly[(N-isopropylacrylamide)-co-(vinyl imidazole)] Microgels for Controlled Lipase Release. Macromolecular Rapid Communications. 2015. vol. 36, no. 21, 1891-1896. DOI: 10.1002/marc.201500311}} @misc{lendlein_editorial_advanced_2015, author={Lendlein, A., Neffe, A.T., Jerome, C.}, title={Editorial: Advanced Functional Polymers in Medicine (AFPM)}, year={2015}, howpublished = {Other: editorial}, doi = {https://doi.org/10.3233/CH-151941}, abstract = {No abstract}, note = {Online available at: \url{https://doi.org/10.3233/CH-151941} (DOI). Lendlein, A.; Neffe, A.; Jerome, C.: Editorial: Advanced Functional Polymers in Medicine (AFPM). Clinical Hemorheology and Microcirculation. 2015. vol. 60, no. 1, 1-2. DOI: 10.3233/CH-151941}} @misc{lwenberg_influence_of_2015, author={Löwenberg, C., Julich-Gruner, K., Neffe, A., Lendlein, A.}, title={Influence of Ions on the Properties of Swollen Gelatin-Based Networks}, year={2015}, howpublished = {conference poster: Galway (IRL);}, note = {Löwenberg, C.; Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Influence of Ions on the Properties of Swollen Gelatin-Based Networks. In: Advanced Functional Polymers for Medicine (AFPM). Galway (IRL). 2015.}} @misc{mathew_effect_of_2015, author={Mathew, S., Baudis, S., Neffe, A.T., Behl, M., Wischke, C., Lendlein, A.}, title={Effect of diisocyanate linkers on the degradation characteristics of copolyester urethanes as potential drug carrier matrices}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.ejpb.2015.03.025}, abstract = {In this study, the effect of three aliphatic diisocyanate linkers, L-lysine diisocyanate ethyl ester (LDI), hexamethylene diisocyanate (HDI), and racemic 2,2,4-/2,4,4-trimethyl hexamethylene diisocyanate (TMDI), on the degradation of oligo[(rac-lactide)-co-glycolide] (64:36 mol%) based polyester urethanes (PEU) was examined. Samples were characterized for their molecular weight, mass loss, water uptake, sequence structure, and thermal and mechanical properties. Compared to non-segmented PLGA, the PEU showed higher water uptake and generally degraded faster. Interestingly, the rate of degradation was not directly correlating with the hydrophilicity of the diisocyanate moieties; instead, competing intra-/intermolecular hydrogen bonds in between urethane moieties appear to substantially decrease the rate of degradation for LDI-derived PEU. By comparing microparticles (μm) and films (mm) as matrices of different dimensions, it was shown that autocatalysis remains a contributor to degradation of the larger-sized PEU matrices as it is typical for non-segmented lactide/glycolide copolymers. The shown capacity of lactide/glycolide-based multiblock copolymers to degrade faster than PLGA and exhibit improved elastic properties could be of interest for medical implants and drug release systems.}, note = {Online available at: \url{https://doi.org/10.1016/j.ejpb.2015.03.025} (DOI). Mathew, S.; Baudis, S.; Neffe, A.; Behl, M.; Wischke, C.; Lendlein, A.: Effect of diisocyanate linkers on the degradation characteristics of copolyester urethanes as potential drug carrier matrices. European Journal of Pharmaceutics and Biopharmaceutics A. 2015. vol. 95, 18-26. DOI: 10.1016/j.ejpb.2015.03.025}} @misc{neffe_one_step_2015, author={Neffe, A.T., Pierce, B.F., Tronci, G., Ma, N., Pittermann, E., Gebauer, T., Frank, O., Schossig, M., Xu, X., Willie, B.M., Forner, M., Ellinghaus, A., Lienau, J., Duda, G.N., Lendlein, A.}, title={One Step Creation of Multifunctional 3D Architectured Hydrogels Inducing Bone Regeneration}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1002/adma.201404787}, abstract = {Structured hydrogels showing form stability and elastic properties individually tailorable on different length scales are accessible in a one-step process. They support cell adhesion and differentiation and display growing pore size during degradation. In vivo experiments demonstrate their efficacy in biomaterial-induced bone regeneration, not requiring addition of cells or growth factors.}, note = {Online available at: \url{https://doi.org/10.1002/adma.201404787} (DOI). Neffe, A.; Pierce, B.; Tronci, G.; Ma, N.; Pittermann, E.; Gebauer, T.; Frank, O.; Schossig, M.; Xu, X.; Willie, B.; Forner, M.; Ellinghaus, A.; Lienau, J.; Duda, G.; Lendlein, A.: One Step Creation of Multifunctional 3D Architectured Hydrogels Inducing Bone Regeneration. Advanced Materials. 2015. vol. 27, no. 10, 1738-1744. DOI: 10.1002/adma.201404787}} @misc{julichgruner_anisotropic_composites_2015, author={Julich-Gruner, K.K., Lendlein, A., Boccaccini, A.R., Neffe, A.T.}, title={Anisotropic Composites of Desaminotyrosine and Desaminotyrosyl Tyrosine Functionalized Gelatin and Bioactive Glass Microparticles}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2015.492}, abstract = {Functionalization of gelatin with desaminotyrosine (DAT) and desamino tyrosyl tyrosine (DATT) has been demonstrated to allow network formation based on non-covalent interactions of the aromatic moieties. Based on the observation that the DAT(T) groups furthermore could interact with hydroxyapatite fillers, here it was investigated whether such interactions of DAT(T) could also be employed to stabilize composites formed by functionalized gelatins and bioactive glass (BG) particles. Because of sedimentation of the BG microparticles during the gelification, anisotropic composites with two distinct layers were formed. The characterization of mechanical properties by tensile tests and rheology showed that all composites of non-functionalized and DAT(T) functionalized gelatins with BG microparticles showed an increased Young’s modulus (E) up to 3 MPa, an increased storage modulus (G’) up to 100 kPa, increased tensile strength (σmax) up to 3.4 MPa, and increased loss modulus (G’’) compared to the pure matrices. As the observed effects were more pronounced in the DAT(T) functionalized gelatins compared to non-functionalized gelatins, and a much increased thermal stability of these composites was found, it is likely that there are binding interactions between the aromatic moieties and the BG microparticles. This effect open opportunities for the further development of this type of gelatin-based composites for bone regeneration applications.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2015.492} (DOI). Julich-Gruner, K.; Lendlein, A.; Boccaccini, A.; Neffe, A.: Anisotropic Composites of Desaminotyrosine and Desaminotyrosyl Tyrosine Functionalized Gelatin and Bioactive Glass Microparticles. MRS Online Proceedings Library. 2015. vol. 1718, 82-87. DOI: 10.1557/opl.2015.492}} @misc{neffe_ultrasoundsensitive_microparticles_2015, author={Neffe, A., Vukićević, R., Lützow, K., Pierce, B., Lendlein, A.}, title={Ultrasound-sensitive microparticles based on poly[(N-isopropylacrylamide)-co-(vinyl imidazole)]}, year={2015}, howpublished = {conference poster: Galway (IRL);}, note = {Neffe, A.; Vukićević, R.; Lützow, K.; Pierce, B.; Lendlein, A.: Ultrasound-sensitive microparticles based on poly[(N-isopropylacrylamide)-co-(vinyl imidazole)]. In: 5. Int. Seminar “Advanced Functional Polymers for Medicine (AFPM)”. Galway (IRL). 2015.}} @misc{krger_biomaterialien_fr_2015, author={Krüger, A., Löwenberg, C., Neffe, A., Roch, T., Schöne, A., Schroeter, M., Viszokai, P., Vuki evi , R., Lendlein, A.}, title={Biomaterialien für die Medizin}, year={2015}, howpublished = {conference lecture: Potsdam (DEU);}, note = {Krüger, A.; Löwenberg, C.; Neffe, A.; Roch, T.; Schöne, A.; Schroeter, M.; Viszokai, P.; Vuki evi, R.; Lendlein, A.: Biomaterialien für die Medizin. Potsdamer Tag der Wissenschaften 2015. Potsdam (DEU), 2015.}} @misc{tzoneva_effect_of_2015, author={Tzoneva, R., Uzunova, V., Apostolova, S., Krüger, A., Neffe, A., Jung, F., Lendlein, A.}, title={Effect of electrical stimulation on vascular endothelial and tumor cells on angiogenic response}, year={2015}, howpublished = {conference poster: Regensburg (DEU);}, note = {Tzoneva, R.; Uzunova, V.; Apostolova, S.; Krüger, A.; Neffe, A.; Jung, F.; Lendlein, A.: Effect of electrical stimulation on vascular endothelial and tumor cells on angiogenic response. In: 34. Jahrestagung der Deutschen Gesellschaft für klinische Mikrozirkulation und Hämorheologie (DGKMH). Regensburg (DEU). 2015.}} @misc{neffe_crosslinking_of_2015, author={Neffe, A., Federico, S., Lendlein, A.}, title={Crosslinking of Collagen Through Molecular Recognition by Biomimetic; Decorin-Derived Peptides}, year={2015}, howpublished = {conference lecture: Boston, MA. (USA);}, note = {Neffe, A.; Federico, S.; Lendlein, A.: Crosslinking of Collagen Through Molecular Recognition by Biomimetic; Decorin-Derived Peptides. MRS Fall Meeting 2015; Symposium H “Multifunctionality in Polymer-Based Materials; Gels and Interfaces”. Boston, MA. (USA), 2015.}} @misc{vukievi_mechanical_properties_2015, author={Vuki evi , R., Neffe, A., Gebauer, T., Xu, X., Ma, N., Lendlein, A.}, title={Mechanical Properties of Architectured Gelatin-Based Hydrogels on Different Hierarchical Levels}, year={2015}, howpublished = {conference poster: Boston, MA. (USA);}, note = {Vuki evi, R.; Neffe, A.; Gebauer, T.; Xu, X.; Ma, N.; Lendlein, A.: Mechanical Properties of Architectured Gelatin-Based Hydrogels on Different Hierarchical Levels. In: MRS Fall Meeting 2015; Symposium H “Multifunctionality in Polymer-Based Materials; Gels and Interfaces”. Boston, MA. (USA). 2015.}} @misc{naolou_oligodepsipeptide_synthesis_2015, author={Naolou, T., Brunacci, N., Neffe, A., Lendlein, A.}, title={Oligodepsipeptide synthesis and formation of submicron particles thereof}, year={2015}, howpublished = {conference poster: Boston, MA. (USA);}, note = {Naolou, T.; Brunacci, N.; Neffe, A.; Lendlein, A.: Oligodepsipeptide synthesis and formation of submicron particles thereof. In: MRS Fall Meeting 2015; Symposium H “Multifunctionality in Polymer-Based Materials; Gels and Interfaces”. Boston, MA. (USA). 2015.}} @misc{neffe_biomaterialien_quo_2015, author={Neffe, A.}, title={Biomaterialien quo vadis? Innovative Materialien für die Biomedizin}, year={2015}, howpublished = {conference lecture: Dresden (DEU);}, note = {Neffe, A.: Biomaterialien quo vadis? Innovative Materialien für die Biomedizin. i-WING 2015 – Vom Material zur Innovation. Dresden (DEU), 2015.}} @misc{neffe_going_beyond_2015, author={Neffe, A.T., Lendlein, A.}, title={Going Beyond Compromises in Multifunctionality of Biomaterials}, year={2015}, howpublished = {journal article}, doi = {https://doi.org/10.1002/adhm.201400724}, abstract = {Prioritizing one function in biomaterial and biomedical device design goes hand in hand with compromises with respect to other functions. Strategies to overcome the limitations of such an approach for realizing novel fields of biomaterial application are critically evaluated to promote interdisciplinary and integrative research.}, note = {Online available at: \url{https://doi.org/10.1002/adhm.201400724} (DOI). Neffe, A.; Lendlein, A.: Going Beyond Compromises in Multifunctionality of Biomaterials. Advanced Healthcare Materials. 2015. vol. 4, no. 5, 642-645. DOI: 10.1002/adhm.201400724}} @misc{schoenwaelder_interaction_of_2014, author={Schoenwaelder, S.M.S., Bally, F., Heinke, L., Azucena, C., Bulut, Oe.D., Heissler, S., Kirschhoefer, F., Gebauer, T.P., Neffe, A.T., Lendlein, A., Brenner-Weiss, G., Lahann, J., Welle, A., Overhage, J., Woell, C.}, title={Interaction of Human Plasma Proteins with Thin Gelatin-Based Hydrogel Films: A QCM-D and ToF-SIMS Study}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1021/bm500750v}, abstract = {In the fields of surgery and regenerative medicine, it is crucial to understand the interactions of proteins with the biomaterials used as implants. Protein adsorption directly influences cell-material interactions in vivo and, as a result, regulates, for example, cell adhesion on the surface of the implant. Therefore, the development of suitable analytical techniques together with well-defined model systems allowing for the detection, characterization, and quantification of protein adsorbates is essential. In this study, a protocol for the deposition of highly stable, thin gelatin-based films on various substrates has been developed. The hydrogel films were characterized morphologically and chemically. Due to the obtained low thickness of the hydrogel layer, this setup allowed for a quantitative study on the interaction of human proteins (albumin and fibrinogen) with the hydrogel by Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D). This technique enables the determination of adsorbant mass and changes in the shear modulus of the hydrogel layer upon adsorption of human proteins. Furthermore, Secondary Ion Mass Spectrometry and principal component analysis was applied to monitor the changed composition of the topmost adsorbate layer. This approach opens interesting perspectives for a sensitive screening of viscoelastic biomaterials that could be used for regenerative medicine.}, note = {Online available at: \url{https://doi.org/10.1021/bm500750v} (DOI). Schoenwaelder, S.; Bally, F.; Heinke, L.; Azucena, C.; Bulut, O.; Heissler, S.; Kirschhoefer, F.; Gebauer, T.; Neffe, A.; Lendlein, A.; Brenner-Weiss, G.; Lahann, J.; Welle, A.; Overhage, J.; Woell, C.: Interaction of Human Plasma Proteins with Thin Gelatin-Based Hydrogel Films: A QCM-D and ToF-SIMS Study. Biomacromolecules. 2014. vol. 15, no. 7, 2398-2406. DOI: 10.1021/bm500750v}} @misc{julichgruner_anisotropic_composites_2014, author={Julich-Gruner, K.K., Lendlein, A., Boccaccini, A.R., Neffe, A.T.}, title={Anisotropic Composites of Desaminotyrosine and Desaminotyrosyl Tyrosine Functionalized Gelatin and Bioactive Glass Microparticles}, year={2014}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Julich-Gruner, K.; Lendlein, A.; Boccaccini, A.; Neffe, A.: Anisotropic Composites of Desaminotyrosine and Desaminotyrosyl Tyrosine Functionalized Gelatin and Bioactive Glass Microparticles. 2014 MRS Fall Meeting & Exhibit. Boston, MA (USA), 2014.}} @misc{ullm_biocompatibility_and_2014, author={Ullm, S., Krueger, A., Tondera, C., Gebauer, T.P., Neffe, A.T., Lendlein, A., Jung, F., Pietzsch, J.}, title={Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.biomaterials.2014.08.023}, abstract = {Hydrogels prepared from gelatin and lysine diisocyanate ethyl ester provide tailorable elastic properties and degradation behavior. Their interaction with human aortic endothelial cells (HAEC) as well as human macrophages (Mɸ) and granulocytes (Gɸ) were explored. The experiments revealed a good biocompatibility, appropriate cell adhesion, and cell infiltration. Direct contact to hydrogels, but not contact to hydrolytic or enzymatic hydrogel degradation products, resulted in enhanced cyclooxygenase-2 (COX-2) expression in all cell types, indicating a weak inflammatory activation in vitro. Only Mɸ altered their cytokine secretion profile after direct hydrogel contact, indicating a comparably pronounced inflammatory activation. On the other hand, in HAEC the expression of tight junction proteins, as well as cytokine and matrix metalloproteinase secretion were not influenced by the hydrogels, suggesting a maintained endothelial cell function. This was in line with the finding that in HAEC increased thrombomodulin synthesis but no thrombomodulin membrane shedding occurred. First in vivo data obtained after subcutaneous implantation of the materials in immunocompetent mice revealed good integration of implants in the surrounding tissue, no progredient fibrous capsule formation, and no inflammatory tissue reaction in vivo. Overall, the study demonstrates the potential of gelatin-based hydrogels for temporal replacement and functional regeneration of damaged soft tissue.}, note = {Online available at: \url{https://doi.org/10.1016/j.biomaterials.2014.08.023} (DOI). Ullm, S.; Krueger, A.; Tondera, C.; Gebauer, T.; Neffe, A.; Lendlein, A.; Jung, F.; Pietzsch, J.: Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties. Biomaterials. 2014. vol. 35, no. 37, 9755-9766. DOI: 10.1016/j.biomaterials.2014.08.023}} @misc{wei_wechselwirkungen_von_2014, author={Wei, Q., Becherer, T., Angioletti-Uberti, S., Dzubiella, J., Wischke, C., Neffe, A.T., Lendlein, A., Ballauff, M., Haag, R.}, title={Wechselwirkungen von Proteinen mit Polymerbeschichtungen und Biomaterialien}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1002/ange.201400546}, abstract = {Die Proteinadsorption gilt als der wichtigste Faktor der Wechselwirkung zwischen polymeren Biomaterialien und Körperflüssigkeiten oder -gewebe. Die Haupteinflussfaktoren auf die Proteinadsorption sind wasservermittelte hydrophobe und Hydratationskräfte sowie elektrostatische Wechselwirkungen. Eine systematische Analyse verschiedener Monolagen führte zur Aufstellung allgemeiner Leitsätze, den sogenannten “Whitesides-Regeln”. Diese Konzepte wurden erfolgreich auf die Entwicklung verschiedener proteinresistenter Oberflächen angewendet und werden kontinuierlich weiterentwickelt, um das Verständnis von Protein-Material-Wechselwirkungen über die bisherigen Grenzen hinaus zu erweitern. Ebenso werden die Theorien zu Proteinadsorptionsmechanismen aufgrund der sich schnell entwickelnden analytischen Technologien fortlaufend verbessert. Ziel dieses Aufsatzes ist die Verbesserung der aufgestellten empirischen Leitlinien im Hinblick auf die theoretischen und analytischen Fortschritte. Dabei werden die aktuellen analytischen Methoden zur Untersuchung mechanistischer Hypothesen und Theorien zu Protein-Oberflächen-Wechselwirkungen besprochen. Ein besonderes Augenmerk liegt auf aktuellen Technologien im Bereich bioinerter und biospezifischer Beschichtungen und ihrer Anwendungen in der Biomedizin.}, note = {Online available at: \url{https://doi.org/10.1002/ange.201400546} (DOI). Wei, Q.; Becherer, T.; Angioletti-Uberti, S.; Dzubiella, J.; Wischke, C.; Neffe, A.; Lendlein, A.; Ballauff, M.; Haag, R.: Wechselwirkungen von Proteinen mit Polymerbeschichtungen und Biomaterialien. Angewandte Chemie. 2014. vol. 126, no. 31, 8138-8169. DOI: 10.1002/ange.201400546}} @misc{julichgruner_synthesis_and_2014, author={Julich-Gruner, K.K., Roch, T., Ma, N., Neffe, A.T., Lendlein, A.}, title={Synthesis and characterization of star-shaped oligo(ethylene glycol) with tyrosine derived moieties under variation of their molecular weight}, year={2014}, howpublished = {conference lecture: Liege (B);}, note = {Julich-Gruner, K.; Roch, T.; Ma, N.; Neffe, A.; Lendlein, A.: Synthesis and characterization of star-shaped oligo(ethylene glycol) with tyrosine derived moieties under variation of their molecular weight. Advanced Functional Polymers for Medicine, AFPM 2014. Liege (B), 2014.}} @misc{lendlein_editorial_advanced_2014, author={Lendlein, A., Neffe, A.T., Jerome, C.}, title={Editorial: Advanced Functional Polymers for Medicine}, year={2014}, howpublished = {Other: editorial}, abstract = {No abstract}, note = {Lendlein, A.; Neffe, A.; Jerome, C.: Editorial: Advanced Functional Polymers for Medicine. Advanced Healthcare Materials. 2014. vol. 3, no. 12, 1939-1940.}} @misc{rijckaert_a_high_2014, author={Rijckaert, B., Neffe, A.T., Roch, T., Gebauer, T., Pierce, B.F., Goers, J., Smink, J.J., Gossen, M., Lendlein, A., Leutz, A.}, title={A High Content Screening Assay for Evaluation of Biomaterial-Mediated Cell Fusion Processes}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201400147}, abstract = {Biomaterials are of increasing importance in regenerative medicine and entail delivery systems in somatic cell therapies, matrices for tissue engineering or tissue regeneration. The evaluation of biomaterial induced biological effects remains a key issue in clinical application. Cell-based assays for potential cytotoxic and immunological responses have been developed but are often inadequate to address cell-type specific responses to biomaterials. To quantitatively monitor attachment, survival, proliferation and fusion-controlled differentiation of osteoclasts (bone resorbing cells), a High Content Screening (HCS) assay has been developed based on osteoclast differentiation of the murine monocytic cell line RAW 264.7. This assay was applied to investigate the influence of degradation products of polymers from gelatin and lysine diisocyanate, which display tailorable mechanical properties and have potential as biomaterials. The data show that the degradation products inhibit formation of multinuclear osteoclasts and suggest a potential support of bone regeneration by suppression of bone resorption.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201400147} (DOI). Rijckaert, B.; Neffe, A.; Roch, T.; Gebauer, T.; Pierce, B.; Goers, J.; Smink, J.; Gossen, M.; Lendlein, A.; Leutz, A.: A High Content Screening Assay for Evaluation of Biomaterial-Mediated Cell Fusion Processes. Macromolecular Symposia. 2014. vol. 346, no. 1, 91-99. DOI: 10.1002/masy.201400147}} @misc{neffe_polyethers_on_2014, author={Neffe, A.T., Ruesten-Lange, M.v., Braune, S., Luetzow, K., Roch, T., Jung, F., Weinhart, M., Haag, R., Lendlein, A.}, title={Polyethers on Porous Polymer Surfaces: Relevant Models to Study Protein Adsorption and Hemocompatibility}, year={2014}, howpublished = {conference poster: Freiburg i. Br. (D);}, note = {Neffe, A.; Ruesten-Lange, M.; Braune, S.; Luetzow, K.; Roch, T.; Jung, F.; Weinhart, M.; Haag, R.; Lendlein, A.: Polyethers on Porous Polymer Surfaces: Relevant Models to Study Protein Adsorption and Hemocompatibility. In: Makromolekulares Kolloquium 2014. Freiburg i. Br. (D). 2014.}} @misc{neffe_crosslinking_of_2014, author={Neffe, A.T., Chua, K., Luetzow, K., Pierce, B.F., Lendlein, A., Abell, A.D.}, title={Crosslinking of gelatin by ring opening metathesis under aqueous conditions - An exploratory study}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1002/pat.3359}, abstract = {Ring-opening metathesis catalysis has received little attention as a means to functionalize or crosslink biopolymers in water since the required catalysts are usually not stable under these conditions. However, biopolymer solubility suggests such a procedure. We show that Grubbs first and second generation catalysts (in emulsion) as well as a water-soluble Hoveyda-Grubbs catalyst can be applied to crosslink glycidyl methacrylated gelatin with norbornene dicarboxylic acid in water by a cross metathesis-ring opening metathesis approach. A mechanistic study suggests that cross metathesis between the functionalized polymer and the cyclic olefin acts as an initiating step for the crosslinking reaction.}, note = {Online available at: \url{https://doi.org/10.1002/pat.3359} (DOI). Neffe, A.; Chua, K.; Luetzow, K.; Pierce, B.; Lendlein, A.; Abell, A.: Crosslinking of gelatin by ring opening metathesis under aqueous conditions - An exploratory study. Polymers for Advanced Technologies. 2014. vol. 25, no. 11, 1371-1375. DOI: 10.1002/pat.3359}} @misc{arya_chondrocyte_redifferentiation_2014, author={Arya, N., Gebauer, T.P., Neffe, A.T., Lendlein, A., Shastri, V.P.}, title={Chondrocyte re-differentiation on ethyl lysine diisocyanate cross-linked gelatin based 3-D scaffolds: Application in cartilage tissue engineering}, year={2014}, howpublished = {conference object: ;}, doi = {https://doi.org/10.1002/term.1932}, abstract = {No abstract}, note = {Online available at: \url{https://doi.org/10.1002/term.1932} (DOI). Arya, N.; Gebauer, T.; Neffe, A.; Lendlein, A.; Shastri, V.: Chondrocyte re-differentiation on ethyl lysine diisocyanate cross-linked gelatin based 3-D scaffolds: Application in cartilage tissue engineering. Journal of Tissue Engineering and Regenerative Medicine. 2014. DOI: 10.1002/term.1932}} @misc{neffe_multivalent_grafting_2014, author={Neffe, A.T., Ruesten-Lange, M.v., Braune, S., Luetzow, K., Roch, T., Richau, K., Krueger, A., Becherer, T., Thuenemann, A.F., Jung, F., Haag, R., Lendlein, A.}, title={Multivalent grafting of hyperbranched oligo- and polyglycerols shielding rough membranes to mediate hemocompatibility}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1039/C4TB00184B}, abstract = {Hemocompatible materials are needed for internal and extracorporeal biomedical applications, which should be realizable by reducing protein and thrombocyte adhesion to such materials. Polyethers have been demonstrated to be highly efficient in this respect on smooth surfaces. Here, we investigate the grafting of oligo- and polyglycerols to rough poly(ether imide) membranes as a polymer relevant to biomedical applications and show the reduction of protein and thrombocyte adhesion as well as thrombocyte activation. It could be demonstrated that, by performing surface grafting with oligo- and polyglycerols of relatively high polydispersity (>1.5) and several reactive groups for surface anchoring, full surface shielding can be reached, which leads to reduced protein adsorption of albumin and fibrinogen. In addition, adherent thrombocytes were not activated. This could be clearly shown by immunostaining adherent proteins and analyzing the thrombocyte covered area. The presented work provides an important strategy for the development of application relevant hemocompatible 3D structured materials.}, note = {Online available at: \url{https://doi.org/10.1039/C4TB00184B} (DOI). Neffe, A.; Ruesten-Lange, M.; Braune, S.; Luetzow, K.; Roch, T.; Richau, K.; Krueger, A.; Becherer, T.; Thuenemann, A.; Jung, F.; Haag, R.; Lendlein, A.: Multivalent grafting of hyperbranched oligo- and polyglycerols shielding rough membranes to mediate hemocompatibility. Journal of Materials Chemistry B. 2014. vol. 2, no. 23, 3626-3635. DOI: 10.1039/C4TB00184B}} @misc{neffe_micellization_of_2014, author={Neffe, A.T., Santan, H.D., Kamlage, S., Gottschalk, B., Lendlein, A.}, title={Micellization of Aminoterminated Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) in the Presence of Hyaluronic Acid}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201400145}, abstract = {At concentrations above 15 wt-%, aqueous solutions of mixtures of aminoterminated poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) block copolymers (PEPE) and hyaluronic acid show a reversible sol-gel-transition upon change of temperature. For non-aminoterminated PEPEs, this gel transition could be related to micellization and subsequent colloidal jamming of the micelles. Here, the mechanism of gelation for the aminoterminated compound is investigated by fluorescence spectroscopy and 1H-NMR spectroscopy. The aminoterminated PEPE also form micelles. The onset of micellization is temperature and concentration dependent, and can be tuned to 20–40 °C. Interestingly, with increasing molecular weight of the hyaluronic acid component, the micellization is observed at lower concentrations.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201400145} (DOI). Neffe, A.; Santan, H.; Kamlage, S.; Gottschalk, B.; Lendlein, A.: Micellization of Aminoterminated Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) in the Presence of Hyaluronic Acid. Macromolecular Symposia. 2014. vol. 345, no. 1, 91-97. DOI: 10.1002/masy.201400145}} @misc{wei_protein_interactions_2014, author={Wei, Q., Becherer, T., Angioletti-Uberti, S., Dzubiella, J., Wischke, C., neffe, A.T., Lendlein, A., Ballauff, M., Haag, R.}, title={Protein Interactions with Polymer Coatings and Biomaterials}, year={2014}, howpublished = {journal article}, doi = {https://doi.org/10.1002/anie.201400546}, abstract = {Protein adsorption is considered to be the most important factor of the interaction between polymeric biomaterials and body fluids or tissues. Water-mediated hydrophobic and hydration forces as well as electrostatic interactions are believed to be the major factors of protein adsorption. A systematic analysis of various monolayer systems has resulted in general guidelines, the so-called “Whitesides rules”. These concepts have been successfully applied for designing various protein-resistant surfaces and are being studied to expand the understanding of protein–material interactions beyond existing limitations. Theories on the mechanisms of protein adsorption are constantly being improved due to the fast-developing analytical technologies. This Review is aimed at improving these empirical guidelines with regard to present theoretical and analytical advances. Current analytical methods to test mechanistic hypotheses and theories of protein–surface interactions will be discussed. Special focus will be given to state-of-the-art bioinert and biospecific coatings and their applications in biomedicine.}, note = {Online available at: \url{https://doi.org/10.1002/anie.201400546} (DOI). Wei, Q.; Becherer, T.; Angioletti-Uberti, S.; Dzubiella, J.; Wischke, C.; neffe, A.; Lendlein, A.; Ballauff, M.; Haag, R.: Protein Interactions with Polymer Coatings and Biomaterials. Angewandte Chemie - International Edition. 2014. vol. 53, no. 31, 8004-8031. DOI: 10.1002/anie.201400546}} @misc{krueger_interaction_between_2014, author={Krueger, A., Ullm, S., Gebauer, T.G., Neffe, A.T., Pietzsch, J., Jung, F., Lendlein, A.}, title={Interaction between human umbilical venous endothelial cell and a gelatin-based hydrogel}, year={2014}, howpublished = {conference lecture: Villingen-Schwenningen (D);}, note = {Krueger, A.; Ullm, S.; Gebauer, T.; Neffe, A.; Pietzsch, J.; Jung, F.; Lendlein, A.: Interaction between human umbilical venous endothelial cell and a gelatin-based hydrogel. 33. Jahrestagung der Deutschen Gesellschaft fuer klinische Mikrozirkulation und Haemorheologie. Villingen-Schwenningen (D), 2014.}} @misc{neffe_combinations_of_2014, author={Neffe, A., Julich-Gruner, K., Lendlein, A.}, title={Combinations of biopolymers and synthetic polymers for bone regeneration}, year={2014}, howpublished = {book part}, abstract = {Critical bone defects do not heal by themselves, but the regeneration process can be supported by biomaterial implants. Combinations of synthetic polymers, intended to provide the required mechanical strength and processability, and biopolymers, giving cells a suitable environment for proliferation and inducing bone growth, has recently drawn attention in order to provide multifunctional implants. In this chapter, the combination of the different polymer classes on the molecular level and by surface functionalization is discussed, with an emphasis on physicochemical properties and the biological functions of the materials as well as future trends in this research field.}, note = {Neffe, A.; Julich-Gruner, K.; Lendlein, A.: Combinations of biopolymers and synthetic polymers for bone regeneration. In: Dubruel, P.; Vlierberghe, S. (Ed.): Biomaterials for Bone Regeneration: Novel Techniques and Applications. Woodhead Publishing. 2014. 87-109.}} @misc{gebauer_single_protein_2014, author={Gebauer, T., Neffe, A.T., Lendlein, A.}, title={Single Protein Adsorption to Hydrogels Obtained from Gelatin and a Diisocyanate}, year={2014}, howpublished = {conference lecture: Villingen-Schwenningen (D);}, note = {Gebauer, T.; Neffe, A.; Lendlein, A.: Single Protein Adsorption to Hydrogels Obtained from Gelatin and a Diisocyanate. 33. Jahrestagung der Deutschen Gesellschaft fuer klinische Mikrozirkulation und Haemorheologie. Villingen-Schwenningen (D), 2014.}} @misc{loewenberg_influence_of_2014, author={Loewenberg, C., Julich-Gruner, K.K., Neffe, A.T, Lendlein, A.}, title={Influence of glycidylmethacrylate functional groups attached to gelatin on the formation and properties of hydrogels}, year={2014}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Loewenberg, C.; Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Influence of glycidylmethacrylate functional groups attached to gelatin on the formation and properties of hydrogels. 2014 MRS Fall Meeting & Exhibit. Boston, MA (USA), 2014.}} @misc{lendlein_design_of_2014, author={Lendlein, A., Behl, M., Neffe, A.T.}, title={Design of Multifuncational Biomaterials by Chemical Integration of Functions}, year={2014}, howpublished = {conference lecture (invited): Nice (F);}, note = {Lendlein, A.; Behl, M.; Neffe, A.: Design of Multifuncational Biomaterials by Chemical Integration of Functions. 2nd International Conference on Bioinspired and Biobased Chemistry and Materials: Nature Inspires Chemical Engineers, NICE 2014. Nice (F), 2014.}} @misc{neffe_mechanic_studies_2014, author={Neffe, A.T., Lendlein, A.}, title={Mechanic Studies of Gelatin Crosslinking}, year={2014}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Neffe, A.; Lendlein, A.: Mechanic Studies of Gelatin Crosslinking. 2014 MRS Fall Meeting & Exhibit. Boston, MA (USA), 2014.}} @misc{racheva_functionalization_degree_2014, author={Racheva, M., Julich-Gruner, K.K., Neffe, A.T., Wischke, C., Lendlein, A.}, title={Functionalization Degree of Telechelics as a Structural Parameter to Influence the Properties of Enzymatically Formed Polyethylene Glycol Based Networks}, year={2014}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Racheva, M.; Julich-Gruner, K.; Neffe, A.; Wischke, C.; Lendlein, A.: Functionalization Degree of Telechelics as a Structural Parameter to Influence the Properties of Enzymatically Formed Polyethylene Glycol Based Networks. 2014 MRS Fall Meeting & Exhibit. Boston, MA (USA), 2014.}} @misc{arya_chondrocyte_redifferentiation_2014, author={Arya, N., Gebauer, T.P., Neffe, A.T., Lendlein, A., Shastri, V.P.}, title={Chondrocyte re-differentiation on ethyl lysine diisocyanate cross-linked gelatin based 3-D scaffolds: Application in cartilage tissue engineering}, year={2014}, howpublished = {conference lecture: Genova (I);}, note = {Arya, N.; Gebauer, T.; Neffe, A.; Lendlein, A.; Shastri, V.: Chondrocyte re-differentiation on ethyl lysine diisocyanate cross-linked gelatin based 3-D scaffolds: Application in cartilage tissue engineering. Tissue Engineering and Regenerative Medicine International Society, Termis 2014. Genova (I), 2014.}} @misc{roch_immunocompatibility_of_2014, author={Roch, T., Julich-Gruner, K.K., Neffe, A.T., Ma, N., Leindlein, A.}, title={Immuno-compatibility of desaminotyrosine and desaminotyrosyl tyrosine functionalized star-shaped oligo(ethylene glycol)s with different molecular weights}, year={2014}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Roch, T.; Julich-Gruner, K.; Neffe, A.; Ma, N.; Leindlein, A.: Immuno-compatibility of desaminotyrosine and desaminotyrosyl tyrosine functionalized star-shaped oligo(ethylene glycol)s with different molecular weights. 2014 MRS Fall Meeting & Exhibit, Symposium B. Boston, MA (USA), 2014.}} @misc{loewenberg_synthesis_and_2014, author={Loewenberg, C., Julich-Gruner, K.K., Neffe, A.T, Lendlein, A.}, title={Synthesis and Characterization of Polymer Networks Based on Glycidylmethacrylated Gelatin}, year={2014}, howpublished = {conference poster: Boston, MA (USA);}, note = {Loewenberg, C.; Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Synthesis and Characterization of Polymer Networks Based on Glycidylmethacrylated Gelatin. In: 2014 MRS Fall Meeting & Exhibit. Boston, MA (USA). 2014.}} @misc{neffe_proteinmaterial_interactions_2014, author={Neffe, A.T., Ober, C.K.}, title={Protein-Material Interactions}, year={2014}, howpublished = {lecture: Boston University, FB Material Science;}, note = {Neffe, A.; Ober, C.: Protein-Material Interactions. Boston University, FB Material Science, 2014.}} @misc{julichgruner_starshaped_oligoethylene_2014, author={Julich-Gruner, K.K., Loewenberg, C., Roch, T., Neffe, A.T., Lendlein, A.}, title={Star-shaped Oligo(Ethylene Glycols) Functionalized with Desaminotyrosine and Desamino Tyrosyl Tyrosine}, year={2014}, howpublished = {conference poster: Montecatini Terme (I);}, note = {Julich-Gruner, K.; Loewenberg, C.; Roch, T.; Neffe, A.; Lendlein, A.: Star-shaped Oligo(Ethylene Glycols) Functionalized with Desaminotyrosine and Desamino Tyrosyl Tyrosine. In: 6th Forum on New Materials: Symposium Smart Polymers for Biomedical Applications, CIMTEC 2014. Montecatini Terme (I). 2014.}} @misc{wischke_a_polymeric_2014, author={Wischke, C., Loebler, M., Neffe, A.T., Hanh, B.D., Sternberg, K., Stachs, O., Guthoff, R., Lendlein, A.}, title={A polymeric multifunctional glaucoma implant}, year={2014}, howpublished = {conference lecture: Frankfurt / M (D);}, note = {Wischke, C.; Loebler, M.; Neffe, A.; Hanh, B.; Sternberg, K.; Stachs, O.; Guthoff, R.; Lendlein, A.: A polymeric multifunctional glaucoma implant. Jahrestagung Deutsche Pharmazeutische Gesellschaft 2014. Frankfurt / M (D), 2014.}} @misc{neffe_end_group_2014, author={Neffe, A.T., Julich-Gruner, K.K., Roch, T., Garcia Cruz, D.M., Naolou, T., Brunacci, N., Lendlein, A.}, title={End group functionalized starshaped oligo(ethylene glycols) and their biological evaluation}, year={2014}, howpublished = {conference poster: Liege (B);}, note = {Neffe, A.; Julich-Gruner, K.; Roch, T.; Garcia Cruz, D.; Naolou, T.; Brunacci, N.; Lendlein, A.: End group functionalized starshaped oligo(ethylene glycols) and their biological evaluation. In: Advanced Functional Polymers for Medicine, AFPM 2014. Liege (B). 2014.}} @misc{wischke_a_polymeric_2014, author={Wischke, C., Neffe, A.T., Loebler, M., Sternberg, K., Stachs, O., Guthoff, R., Lendlein, A.}, title={A polymeric multifunctional glaucoma implant}, year={2014}, howpublished = {conference poster: Egmond aan Zee (NL);}, note = {Wischke, C.; Neffe, A.; Loebler, M.; Sternberg, K.; Stachs, O.; Guthoff, R.; Lendlein, A.: A polymeric multifunctional glaucoma implant. In: 13th European Symposium on Controlled Drug Delivery, ESCDD 2014. Egmond aan Zee (NL). 2014.}} @misc{neffe_biopolymerbased_hydrogels_2014, author={Neffe, A.T., Lendlein, A.}, title={Biopolymer-based hydrogels and their evaluation as biomaterials}, year={2014}, howpublished = {conference lecture: Liege (B);}, note = {Neffe, A.; Lendlein, A.: Biopolymer-based hydrogels and their evaluation as biomaterials. Advanced Functional Polymers for Medicine, AFPM 2014. Liege (B), 2014.}} @misc{neffe_multifunktionale_polymere_2014, author={Neffe, A.T., Lendlein, A.}, title={Multifunktionale Polymere - Zukunftsperspektiven fuer Materialien in der Medizin}, year={2014}, howpublished = {conference lecture: Teltow (D);}, note = {Neffe, A.; Lendlein, A.: Multifunktionale Polymere - Zukunftsperspektiven fuer Materialien in der Medizin. Vortrag im Industriemuseum Teltow. Teltow (D), 2014.}} @misc{julichgruner_recent_trends_2013, author={Julich-Gruner, K.K., Loewenberg, C., Neffe, A.T., Behl, M., Lendlein, A.}, title={Recent Trends in the Chemistry of Shape-Memory Polymers}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1002/macp.201200607}, abstract = {Shape-memory polymers (SMPs) are stimuli-sensitive materials capable of performing complex movements on demand, which makes them interesting candidates for various applications, for example, in biomedicine or aerospace. This trend article highlights current approaches in the chemistry of SMPs, such as tailored segment chemistry to integrate additional functions and novel synthetic routes toward permanent and temporary netpoints. Multiphase polymer networks and multimaterial systems illustrate that SMPs can be constructed as a modular system of different building blocks and netpoints. Future developments are aiming at multifunctional and multistimuli-sensitive SMPs.}, note = {Online available at: \url{https://doi.org/10.1002/macp.201200607} (DOI). Julich-Gruner, K.; Loewenberg, C.; Neffe, A.; Behl, M.; Lendlein, A.: Recent Trends in the Chemistry of Shape-Memory Polymers. Macromolecular Chemistry and Physics. 2013. vol. 214, no. 5, 527-536. DOI: 10.1002/macp.201200607}} @misc{krueger_influence_of_2013, author={Krueger, A., Goers, J., Zaupa, A., Loewenberg, C., Pierce, B.F., Wischke, C., Neffe, A.T., Jung, F., Lendlein, A.}, title={Influence of physically crosslinked gelatins on the vasculature in the avian chorioallantoic membrane}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-131697}, abstract = {Gelatins functionalized with desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT) form physically crosslinked hydrogels, due to the interactions between the introduced aromatic moieties and gelatin triple helices, whose extent depends on the thermal treatment of the material. The G-modulus of these hydrogels can be tailored to the range of the natural extracellular matrix by adjusting the degree of crosslinking. While these gelatin-based materials have been shown to be not angiogenic, the aim of the study was to evaluate whether these biomaterials influence the regulation of blood vessels when positioned on the chorionallantoic membrane (CAM) of fertilized eggs. The results clearly indicate that the DAT-functionalized gelatin led to an increase of the diameter of the blood vessels in the CAM, which at the same time is probably associated with an increased blood flow in these CAM vessels. The vessel diameters of the four groups (DAT-functionalized gelatin, DATT-functionalized gelatin, plain gelatin, control group without gelatin, each n = 10) differed significantly (p < 0.0001). Vessels in the CAM exposed to the DAT-functionalized gelatin showed with 36.4 μm ± 3.4 μm the largest mean diameters compared to the mean diameters of the samples exposed to DATT gelatin (16.0 μm ± 0.8 μm; p < 0.05) and the plain gelatin (21.2 μm ± 1.0 μm; p < 0.05), which both did not differ significantly from the vessels of the control group. The biocompatibility of the materials in vitro motivates the exploration of their application as matrix in local drug-release systems with short half-life times (one hour up to several days).}, note = {Online available at: \url{https://doi.org/10.3233/CH-131697} (DOI). Krueger, A.; Goers, J.; Zaupa, A.; Loewenberg, C.; Pierce, B.; Wischke, C.; Neffe, A.; Jung, F.; Lendlein, A.: Influence of physically crosslinked gelatins on the vasculature in the avian chorioallantoic membrane. Clinical Hemorheology and Microcirculation. 2013. vol. 55, no. 1, 133-142. DOI: 10.3233/CH-131697}} @misc{braune_dynamic_in_2013, author={Braune, S., Ruesten-Lange, M.v., Mrowietz, C., Luetzow, K., Roch, T., Neffe, A.T., Lendlein, A., Jung, F.}, title={Dynamic in vitro hemocompatibility testing of poly(ether imide) membranes functionalized with linear, methylated oligoglycerol and oligo(ethylene glycol)}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-131729}, abstract = {Linear, side-chain methylated oligoglycerols (OGMe) were recently reported as potential surface passivating molecules for improving the protein resistance of cardiovascular application relevant poly(ether imide) (PEI) membranes. A previously reported in vitro screening under static test conditions allowed an end-point evaluation of the adhesion and activation of adherent thrombocytes performed on the material surfaces and revealed similar levels of thrombogenicity on PEI membranes, functionalized with OGMe and oligo(ethylene glycol) (OEG) of similar molecular weight (Mn = 1,300 g·mol−1 - 1,800 g·mol−1). In the present study, we investigated the hemocompatibility of these materials in a dynamic closed loop system, in order to study time-dependent thrombocyte material interactions also of the circulating thrombocytes by mimicking in vivo relevant flow conditions in a dynamic test system with multiple material contacts. Activation and aggregation of circulating thrombocytes as well as complement activation and plasmatic coagulation were evaluated after 40 circulations of thrombocyte rich plasma in the closed loop system. The results of the dynamic tests revealed no differences between the OGMe and OEG functionalized PEI membranes. Furthermore, no differences were observed between the latter and a PEI membrane treated under the conditions of functionalization at pH 11 (PEI-pH11) without an oligoether being present. Blood plasma protein adsorption, as well as activation, and adherence of circulating thrombocytes occurred in a comparable, but minor manner on all investigated PEI membranes. From this we conclude that the OGMe and OEG surface functionalization did not lead to an improvement of the already good hemocompatibility of the PEI-pH11 membrane.}, note = {Online available at: \url{https://doi.org/10.3233/CH-131729} (DOI). Braune, S.; Ruesten-Lange, M.; Mrowietz, C.; Luetzow, K.; Roch, T.; Neffe, A.; Lendlein, A.; Jung, F.: Dynamic in vitro hemocompatibility testing of poly(ether imide) membranes functionalized with linear, methylated oligoglycerol and oligo(ethylene glycol). Clinical Hemorheology and Microcirculation. 2013. vol. 54, no. 3, 235-248. DOI: 10.3233/CH-131729}} @misc{wischke_sustained_release_2013, author={Wischke, C., Schneider, C., Neffe, A.T., Lendlein, A.}, title={Sustained release hydrogels by in situ polymerized polyalkylcyanoacrylate coating}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jconrel.2013.08.052}, abstract = {No abstract}, note = {Online available at: \url{https://doi.org/10.1016/j.jconrel.2013.08.052} (DOI). Wischke, C.; Schneider, C.; Neffe, A.; Lendlein, A.: Sustained release hydrogels by in situ polymerized polyalkylcyanoacrylate coating. Journal of Controlled Release. 2013. vol. 172, no. 1, e 22. DOI: 10.1016/j.jconrel.2013.08.052}} @misc{gebauer_protein_adsorption_2013, author={Gebauer, T., Neffe, A.T., Lendlein, A.}, title={Protein Adsorption to Gelatin-based Biomaterials}, year={2013}, howpublished = {conference lecture: Teltow (D);}, note = {Gebauer, T.; Neffe, A.; Lendlein, A.: Protein Adsorption to Gelatin-based Biomaterials. HVI Method Workshop 2013. Teltow (D), 2013.}} @misc{santan_protein_adsorption_2013, author={Santan, H., Wischke, C., Neffe, A., Lendlein, A.}, title={Protein Adsorption to Synthetic Polymers}, year={2013}, howpublished = {conference lecture: Teltow (D);}, note = {Santan, H.; Wischke, C.; Neffe, A.; Lendlein, A.: Protein Adsorption to Synthetic Polymers. HVI Method Workshop 2013. Teltow (D), 2013.}} @misc{neffe_biopolymerbased_biomaterials_2013, author={Neffe, A.T., Lendlein, A.}, title={Biopolymer-based Biomaterials}, year={2013}, howpublished = {conference lecture: Teltow (D);}, note = {Neffe, A.; Lendlein, A.: Biopolymer-based Biomaterials. Eroeffnung der Graduiertenschule MacroBio. Teltow (D), 2013.}} @misc{neffe_controlling_mechanical_2013, author={Neffe, A.T., Gebauer, T., Pierce, B.F., Lendlein, A.}, title={Controlling Mechanical Properties of Biopolymer-based Materials}, year={2013}, howpublished = {conference lecture: San Francisco, CA (USA);}, note = {Neffe, A.; Gebauer, T.; Pierce, B.; Lendlein, A.: Controlling Mechanical Properties of Biopolymer-based Materials. 2013 MRS Spring Meeting and Exhibit. San Francisco, CA (USA), 2013.}} @misc{neffe_tailoring_the_2013, author={Neffe, A.T., Gebauer, T., Lendlein, A.}, title={Tailoring the elastic properties and protein adsorption of gelatin-based networks by crosslinking with diisocyanates of differing reactivity and solubility in water}, year={2013}, howpublished = {conference lecture (invited): Berlin (D);}, note = {Neffe, A.; Gebauer, T.; Lendlein, A.: Tailoring the elastic properties and protein adsorption of gelatin-based networks by crosslinking with diisocyanates of differing reactivity and solubility in water. 12th International Conference on Polymers for Advanced Technologies, PAT 2013. Berlin (D), 2013.}} @misc{wischke_polyalkylcyanoacrylates_as_2013, author={Wischke, C., Schneider, C., Neffe, A.T., Lendlein, A.}, title={Polyalkylcyanoacrylates as in situ formed diffusion barriers in multimaterial drug carriers}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jconrel.2013.02.013}, abstract = {Polymeric hydrogels typically release their drug payload rapidly due to their high water content and the diffusivity for drug molecules. This study proposes a multimaterial system to sustain the release by covering the hydrogel with a poly(alkyl-2-cyanoacrylate) [PACA]-based film, which should be formed by an in situ polymerization on the hydrogel surface initiated upon contact with water. A series of PACA-hydrogel hybrid systems with increasing PACA side chain hydrophobicity was prepared using physically crosslinked alginate films and hydrophilic diclofenac sodium as model hydrogel/drug system. Successful synthesis of PACA at the hydrogel surface was confirmed and the PACA layer was identified to be most homogeneous for poly(n-butyl-2-cyanoacrylate) on both the micro- and nanolevel. At the same time, the diclofenac release from the hybrid systems was substantially sustained from ~ 1 day for unmodified hydrogels up to > 14 days depending on the type of PACA employed as diffusion barrier. Overall, in situ polymerized PACA films on hydrogels may be widely applicable to various hydrogel matrices, different matrix sizes as well as more complex shaped hydrogel carriers.}, note = {Online available at: \url{https://doi.org/10.1016/j.jconrel.2013.02.013} (DOI). Wischke, C.; Schneider, C.; Neffe, A.; Lendlein, A.: Polyalkylcyanoacrylates as in situ formed diffusion barriers in multimaterial drug carriers. Journal of Controlled Release. 2013. vol. 169, no. 3, 321-328. DOI: 10.1016/j.jconrel.2013.02.013}} @misc{tripodo_efficient_synthesis_2013, author={Tripodo, G., Wischke, C., Neffe, A.T., Lendlein, A.}, title={Efficient synthesis of pure monotosylated beta-cyclodextrin and its dimers}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.carres.2013.08.018}, abstract = {6-O-Monotosyl-β-cyclodextrin (mono-Ts-βCD) is one of the most important intermediates in the production of substituted βCD. So far, performing the monotosylation reaction and, in particular, the purification steps was challenging, relied on toxic solvents, and resulted in long and expensive procedures at, importantly, low yields. Here, the reaction of cyclodextrin with p-toluenesulfonyl chloride in aqueous environment is described to obtain a highly pure mono-Ts-βCD, for which a single-step purification with a cation exchange resin was applied. With this synthetic route and purification, yields could be increased from typically <10–15% to 35%, and organic solvents could be avoided. As characterized by FTIR, mass spectrometry, elemental analysis, and NMR, mono-Ts-βCD was obtained with a molar purity of >98 mol %. From mono-Ts-βCD, β-cyclodextrin dimers linked by ethylenediamine (bis-Et-βCD) were successfully prepared (yield 93%, purity 96 mol %) in a one-step approach using an anion exchange resin to trap leaving groups that typically interfere in the reaction. This synthesis procedure with a direct collection of side-products may be a general strategy applicable for nucleophilic substitution of tosylated cyclodextrins.}, note = {Online available at: \url{https://doi.org/10.1016/j.carres.2013.08.018} (DOI). Tripodo, G.; Wischke, C.; Neffe, A.; Lendlein, A.: Efficient synthesis of pure monotosylated beta-cyclodextrin and its dimers. Carbohydrate Research. 2013. vol. 381, 59-63. DOI: 10.1016/j.carres.2013.08.018}} @misc{julichgruner_immunological_investigations_2013, author={Julich-Gruner, K.K., Roch, T., Ma, N., Neffe, A.T., Lendlein, A.}, title={Immunological investigations of oligoethylene glycols functionalized with desaminotyrosine and desaminotyrosyltyrosine}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2013.831}, abstract = {Biomaterials require thorough in vitro testing before being applied in vivo. Unwanted contaminations of biomaterials but also their intrinsic properties can cause uncontrolled immune response leading to severe consequences for the patient. Therefore, immunological evaluation of materials for biomedical applications is mandatory before entering clinical application. In order to introduce physical netpoints, the aromatic compounds desaminotyrosine (DAT) and desaminotyrosyl-tyrosine (DATT) were successfully used to functionalize linear and star-shaped oligoethylene glycol (lOEG and sOEG) as previously described. The materials showed properties of surfactants and have potential to be used for solubilization of lipophilic drugs in water. Furthermore, the materials are susceptible for H2O2 degradation as determined by MALDI-ToF MS analyses. This is important for potential in vivo applications, as macrophages can release reactive oxygen species (ROS) under inflammatory conditions. As it is known that surfactant solutions of high concentration can lead to cell lysis, the effects of OEG-DAT(T) solutions on murine RAW macrophages were investigated. Even at highest OEG-DAT(T) concentration of 1000 µg·mL-1 the viability of the RAW cells was not significantly impaired. Additionally, the polymers were incubated with whole human blood and the production of inflammatory cytokines such as the tumor necrosis factor (TNF)-α and interleukin (IL)-6 was determined. Only at high concentrations, the OEG-DAT(T) solution induced low levels of TNF-α and IL-6, indicating that a mild inflammatory reaction could be expected when such high OEG-DAT(T) concentrations are applied in vivo. Similarly, the OEG-DAT(T) solution did not induce ROS in monocytes and neutrophils after incubation with whole human blood. Conclusively, the data presented here demonstrate that OEG-DAT(T) do not lead to a substantial activation of the innate immune mechanisms and could therefore be investigated for solubilizing pharmaceutical agents.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2013.831} (DOI). Julich-Gruner, K.; Roch, T.; Ma, N.; Neffe, A.; Lendlein, A.: Immunological investigations of oligoethylene glycols functionalized with desaminotyrosine and desaminotyrosyltyrosine. MRS Online Proceedings Library. 2013. vol. 1569, 9-14. DOI: 10.1557/opl.2013.831}} @misc{julichgruner_synthesis_and_2013, author={Julich-Gruner, K.K., Roch, T., Ma, N., Neffe, A.T., Lendlein, A.}, title={Synthesis and biological evaluation of oligoethylene glycol materials functionalized with desaminotyrosine and desaminotyrosyltyrosine}, year={2013}, howpublished = {conference lecture: San Francisco, CA (USA);}, note = {Julich-Gruner, K.; Roch, T.; Ma, N.; Neffe, A.; Lendlein, A.: Synthesis and biological evaluation of oligoethylene glycol materials functionalized with desaminotyrosine and desaminotyrosyltyrosine. 2013 MRS Spring Meeting and Exhibit, Symposium MM – Advanced Materials for Biological and Biomedical Applications. San Francisco, CA (USA), 2013.}} @misc{gebauer_influence_of_2013, author={Gebauer, T., Neffe, A.T., Lendlein, A.}, title={Influence of diisocyanate reactivity and water solubility on the formation of gelatin-based networks in water}, year={2013}, howpublished = {conference poster: San Francisco, CA (USA);}, note = {Gebauer, T.; Neffe, A.; Lendlein, A.: Influence of diisocyanate reactivity and water solubility on the formation of gelatin-based networks in water. In: 2013 MRS Spring Meeting and Exhibit, Symposium MM – Advanced Materials for Biological and Biomedical Applications. San Francisco, CA (USA). 2013.}} @misc{ullm_gelatinbased_biomaterials_2013, author={Ullm, S., Tondera, C., Gebauer, T., Neffe, A.T., Lendlein, A., Pietzsch, J.}, title={Gelatin-based biomaterials with tailorable mechanical properties as promising matrices for soft tissue replacement}, year={2013}, howpublished = {conference poster: Leipzig (D);}, note = {Ullm, S.; Tondera, C.; Gebauer, T.; Neffe, A.; Lendlein, A.; Pietzsch, J.: Gelatin-based biomaterials with tailorable mechanical properties as promising matrices for soft tissue replacement. In: World Conference on Regenerative Medicine, WCRM 2013. Leipzig (D). 2013.}} @misc{wischke_the_suitability_2013, author={Wischke, C., Neffe, A.T., Ruesten-Lange, M.v., Braune, S., Luetzow, K., Roch, T., Richau, K., Jung, F., Lendlein, A.}, title={The Suitability of Polyethers to Reduce Protein Adsorption and to Improve Hemocompatibility}, year={2013}, howpublished = {conference lecture: Berlin (D);}, note = {Wischke, C.; Neffe, A.; Ruesten-Lange, M.; Braune, S.; Luetzow, K.; Roch, T.; Richau, K.; Jung, F.; Lendlein, A.: The Suitability of Polyethers to Reduce Protein Adsorption and to Improve Hemocompatibility. 12th International Conference on Polymers for Advanced Technologies, PAT 2013. Berlin (D), 2013.}} @misc{neffe_biopolymers_master_2013, author={Neffe, A., Lendlein, A.}, title={Biopolymers; Master of Polymer Science - Advanced Topics}, year={2013}, howpublished = {lecture: Freie Universität Berlin;}, note = {Neffe, A.; Lendlein, A.: Biopolymers; Master of Polymer Science - Advanced Topics. Freie Universität Berlin, 2013.}} @misc{racheva_influence_of_2013, author={Racheva, M., Julich-Gruner, K.K., Nöchel, U., Neffe, A.T., Wischke, C., Lendlein, A.}, title={Influence of Drying Procedures on Network Formation and Properties of Hydrogels from Functionalized Gelatin}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201300112}, abstract = {Side chain functionalization of gelatin with tyrosine-derived moieties, desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT), has been reported to lead to physical networks stabilized by aromatic interactions and hydrogen bonds, while the inherent ability of gelatin chains to organize in helices is suppressed. Here, the treatment of DAT and DATT gelatin films at defined temperatures (drying at 5 °C, freeze-drying, and freeze-thawing) were explored for the potential to additionally stabilize the hydrogels by increasing the content of helical domains as additional physical netpoints. The influence of the drying procedures on the hydrogel properties such as network morphology and mechanical properties were analyzed by WAXS, swelling, and rheological measurements. The triple helix content had a stabilizing effect on gelatin-based hydrogels at temperatures below the helix-to-coil transition. However, this effect was less pronounced at physiological conditions above the transition temperature, resulting in rapid dissolution of the physical gelatin networks.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201300112} (DOI). Racheva, M.; Julich-Gruner, K.; Nöchel, U.; Neffe, A.; Wischke, C.; Lendlein, A.: Influence of Drying Procedures on Network Formation and Properties of Hydrogels from Functionalized Gelatin. Macromolecular Symposia. 2013. vol. 334, no. 1, 24-32. DOI: 10.1002/masy.201300112}} @misc{lendlein_preface_advanced_2013, author={Lendlein, A., Neffe, A.T.}, title={Preface: Advanced Functional Polymers in Medicine}, year={2013}, howpublished = {Other: editorial}, doi = {https://doi.org/10.1002/masy.201370041}, abstract = {This volume of Macromolecular Symposia is the conference proceeding volume from the 533th WE-Heraeus Seminar “Advanced Functional Polymers for Medicine” held in Bad Honnef, Germany, from May 27 to May 29, 2013. The meeting was organized by Andreas Lendlein and Axel T. Neffe as the third conference in the annual Advanced Functional Polymers for Medicine conference series}, note = {Online available at: \url{https://doi.org/10.1002/masy.201370041} (DOI). Lendlein, A.; Neffe, A.: Preface: Advanced Functional Polymers in Medicine. Macromolecular Symposia. 2013. vol. 334, no. 1, 8-9. DOI: 10.1002/masy.201370041}} @misc{neffe_progress_in_2013, author={Neffe, A.T., Wischke, C., Racheva, M., Lendlein, A.}, title={Progress in biopolymer-based biomaterials and their application in controlled drug delivery}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1586/17434440.2013.839209}, abstract = {Biopolymer-based materials are based on re-growing resources and are attractive for biomedical applications, as they can inherently combine degradability in vivo, can offer sites of adhesion for cells and proteins, often show good biocompatibility and may additionally be used to release embedded bioactive molecules. However, their selection and efficient use for specific applications require an understanding of molecular principles and relationships between the molecular and macroscopic level to establish distinct properties and functions. Here, synthetic routes are described, which allow tailoring properties and functions of biopolymer-based materials. The biological evaluation of such materials is discussed, with a special emphasis on their application in controlled release systems such as hydrogels and particulate carriers.}, note = {Online available at: \url{https://doi.org/10.1586/17434440.2013.839209} (DOI). Neffe, A.; Wischke, C.; Racheva, M.; Lendlein, A.: Progress in biopolymer-based biomaterials and their application in controlled drug delivery. Expert Review of Medical Devices. 2013. vol. 10, no. 6, 813-833. DOI: 10.1586/17434440.2013.839209}} @misc{wischke_a_multifunctional_2013, author={Wischke, C., Neffe, A.T., Hanh, B.D., Kreiner, C.F., Sternberg, K., Stachs, O., Guthoff, R.F., Lendlein, A.}, title={A multifunctional bilayered microstent as glaucoma drainage device}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jconrel.2013.10.021}, abstract = {Commercial non-degradable glaucoma implants are often associated with undesired hypotony, fibrosis, long term failure, and damage of adjacent tissues, which may be overcome by a multifunctional polymeric microstent for suprachoroidal drainage. This study reports the design and fabrication of such devices with tailorable internal diameters (50–300 μm) by solvent-free, continuous hot melt extrusion from blends of poly[(ε-caprolactone)-co-glycolide] and poly(ε-caprolactone) [PCL]. A spatially directed release was supported by bilayered microstents with an internal drug-free PCL layer, and a quantitative description of release kinetics with diclofenac sodium as model drug was provided. Furthermore, the slow degradation pattern (> 1 year) was analyzed and potential effects of 1–5 wt.% drug loading on material properties were excluded. Translational aspects including sterilization by γ-irradiation on dry ice, in vitro biocompatibility, and in vivo implantation were addressed. The promising results support further functional analysis of long-term in vivo performance and suppression of disadvantageous capsule formation.}, note = {Online available at: \url{https://doi.org/10.1016/j.jconrel.2013.10.021} (DOI). Wischke, C.; Neffe, A.; Hanh, B.; Kreiner, C.; Sternberg, K.; Stachs, O.; Guthoff, R.; Lendlein, A.: A multifunctional bilayered microstent as glaucoma drainage device. Journal of Controlled Release. 2013. vol. 172, no. 3, 1002-1010. DOI: 10.1016/j.jconrel.2013.10.021}} @misc{neffe_polyethylene_glycol_2013, author={Neffe, A.T., Von Ruesten-Lange, M., Braune, S., Luetzow, K., Roch, T., Richau, K., Jung, F., Lendlein, A.}, title={Poly(ethylene glycol) Grafting to Poly(ether imide) Membranes: Influence on Protein Adsorption and Thrombocyte Adhesion}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1002/mabi.201300309}, abstract = {The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) membranes by grafting with monoamino PEG of different chain lengths (Mn = 1 kDa or 10 kDa) and end groups (methoxy or hydroxyl) is proven by spectroscopy, changes of surface hydrophilicity, and surface shielding effects. The surface functionalization does lead to reduction of adsorption of BSA, but not of fibrinogen. The thrombocyte adhesion is increased compared to untreated PEI surfaces. Conclusively, rough instead of smooth polymer or gold surfaces should be investigated as relevant models.}, note = {Online available at: \url{https://doi.org/10.1002/mabi.201300309} (DOI). Neffe, A.; Von Ruesten-Lange, M.; Braune, S.; Luetzow, K.; Roch, T.; Richau, K.; Jung, F.; Lendlein, A.: Poly(ethylene glycol) Grafting to Poly(ether imide) Membranes: Influence on Protein Adsorption and Thrombocyte Adhesion. Macromolecular Bioscience. 2013. vol. 13, no. 12, 1720-1729. DOI: 10.1002/mabi.201300309}} @misc{gebauer_influence_of_2013, author={Gebauer, T., Neffe, A.T., Lendlein, A.}, title={Influence of diisocyanate reactivity and water solubility on the formation and the mechanical properties of gelatin-based networks in water}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2013.839}, abstract = {Gelatin can be covalently crosslinked in aqueous solution by application of diisocyanates like L-lysine diisocyanate ethyl ester in order to form hydrogels. Reaction of isocyanate groups with water is however a limiting factor in hydrogel network formation and can strongly influence the outcome of the crosslinking process. Here, diisocyanates with different water solubility and reactivity were applied for the formation of gelatin-based hydrogel networks and the mechanical properties of the hydrogels were investigated to gain a better understanding of starting material/ hydrogel property relations. L-Lysin diisocyanate ethyl ester (LDI), 2, 4-toluene diisocyanate (TDI), 1, 4-butane diisocyanate (BDI), and isophorone diisocyanate (IPDI) were selected, having different solubility in water ranging from 10-4 to 10-2 mol·L-1. BDI and LDI were estimated to have average reactive isocyanates groups, whereas TDI is highly reactive and IPDI has low reactivity. Formed hydrogels showed different morphologies and were partially very inhomogeneous. Gelation time (1 to 50 minutes), water uptake (300 to 900 wt.-%), and mechanical properties determined by tensile tests (E-moduli 35 to 370 kPa) and rheology (Shear moduli 4.5 to 19.5 kPa) showed that high water solubility as well as high reactivity leads to the formation of poorly crosslinked or inhomogeneous materials. Nevertheless, diisocyanates with lower solubility in water and low reactivity are able to form stable, homogeneous hydrogel networks with gelatin in water.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2013.839} (DOI). Gebauer, T.; Neffe, A.; Lendlein, A.: Influence of diisocyanate reactivity and water solubility on the formation and the mechanical properties of gelatin-based networks in water. MRS Online Proceedings Library. 2013. vol. 1569, 15-20. DOI: 10.1557/opl.2013.839}} @misc{neffe_tailoring_of_2013, author={Neffe, A.T., Gebauer, T., Lendlein, A.}, title={Tailoring of Mechanical Properties of Diisocyanate Crosslinked Gelatin-Based Hydrogels}, year={2013}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2013.837}, abstract = {Polymer network formation is an important tool for tailoring mechanical properties of polymeric materials. One option to synthesize a network is the addition of bivalent crosslinkers reacting with functional groups present in a polymer. In case of polymer network syntheses based on biopolymers, performing such a crosslinking reaction in water is sometimes necessary in view of the solubility of the biopolymer, such as gelatin, and can be beneficial to avoid potential contamination of the formed material with organic solvents in view of applications in biomedicine. In the case of applying diisocyanates for the crosslinking in water, it is necessary to show that the low molecular weight bifunctional crosslinker has fully reacted, while tailoring of the mechanical properties of the resulting hydrogels is possible despite the complex reaction mechanism. Here, the formation of gelatin-based hydrogel networks with the diisocyanates 2, 4-toluene diisocyanate, 1, 4-butane diisocyanate, and isophorone diisocyanate is presented. It is shown that extensive washing of materials is required to ensure full conversion of the diisocyanates. The use of different diisocyanates gives hydrogels covering a large range of Young’s moduli (12-450 kPa). The elongations at break (up to 83%) as well as the maximum tensile strengths (up to 410 kPa) of the hydrogels described here are much higher than for lysine diisocyanate ethyl ester crosslinked gelatin reported before. Rheological investigations suggest that the network formation in some cases is due to physical interactions and entanglements rather than covalent crosslink formation.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2013.837} (DOI). Neffe, A.; Gebauer, T.; Lendlein, A.: Tailoring of Mechanical Properties of Diisocyanate Crosslinked Gelatin-Based Hydrogels. MRS Online Proceedings Library. 2013. vol. 1569, 3-8. DOI: 10.1557/opl.2013.837}} @misc{lendlein_advanced_functional_2013, author={Lendlein, A., Neffe, A.T.}, title={Advanced Functional Polymers for Medicine}, year={2013}, howpublished = {Other: editorial}, doi = {https://doi.org/10.1002/mabi.201300507}, abstract = {The application of polymers in medicine requires tailored properties and functions. While properties are material inherent (e.g. thermal transitions, mechanical properties), functions can only be realized by combining a certain molecular structure with physical principles, such as the shape-memory effect based on entropy elasticity of a network and suitable phase transitions, while drug release is ruled by diffusion and interactions at interfaces.}, note = {Online available at: \url{https://doi.org/10.1002/mabi.201300507} (DOI). Lendlein, A.; Neffe, A.: Advanced Functional Polymers for Medicine. Macromolecular Bioscience. 2013. vol. 13, no. 12, 1639-1639. DOI: 10.1002/mabi.201300507}} @misc{racheva_influence_of_2013, author={Racheva, M., Julich-Gruner, K., Noechel, U., Neffe, A.T., Wischke, C., Lendlein, A.}, title={Influence of drying procedures on network properties of physical gelatin hydrogels}, year={2013}, howpublished = {conference poster: Bad Honnef (D);}, note = {Racheva, M.; Julich-Gruner, K.; Noechel, U.; Neffe, A.; Wischke, C.; Lendlein, A.: Influence of drying procedures on network properties of physical gelatin hydrogels. In: Advanced Functional Polymers for Medicine, 533th WE-Heraeus-Seminar. Bad Honnef (D). 2013.}} @misc{gebauer_influence_of_2013, author={Gebauer, T., Neffe, A.T., Lendlein, A.}, title={Influence of diisocyanate reactivity and water solubility on the formation of gelatin-based networks in water}, year={2013}, howpublished = {conference poster: Potsdam (D);}, note = {Gebauer, T.; Neffe, A.; Lendlein, A.: Influence of diisocyanate reactivity and water solubility on the formation of gelatin-based networks in water. In: 28. Tag der Chemie. Potsdam (D). 2013.}} @misc{ma_a_threedimensional_2013, author={Ma, N., Luetzow, C., Kratz, K., Furlani, D., Li, W., Wang, W., Pittermann, E., Neffe, A.T., Sauter, T., Jung, F., Lendlein, A.}, title={A three-dimensional stem cell culture system by polyurethane scaffold material}, year={2013}, howpublished = {conference lecture: Pecs (H);}, note = {Ma, N.; Luetzow, C.; Kratz, K.; Furlani, D.; Li, W.; Wang, W.; Pittermann, E.; Neffe, A.; Sauter, T.; Jung, F.; Lendlein, A.: A three-dimensional stem cell culture system by polyurethane scaffold material. 17th Conference of the European Society for Clinical Hemorheology and Microcirculation, ESCHM 2013. Pecs (H), 2013.}} @misc{rijckaert_a_high_2013, author={Rijckaert, B., Neffe, A.T., Roch, T., Gebauer, T., Pierce, B.F., Goers, J., Smink, J.J., Gossen, M., Lendlein, A., Leutz, A.}, title={A High Content Screening Assay for Evaluation of Biomaterial-Mediated Cell Fusion Processes}, year={2013}, howpublished = {conference lecture: Berlin (D);}, note = {Rijckaert, B.; Neffe, A.; Roch, T.; Gebauer, T.; Pierce, B.; Goers, J.; Smink, J.; Gossen, M.; Lendlein, A.; Leutz, A.: A High Content Screening Assay for Evaluation of Biomaterial-Mediated Cell Fusion Processes. 12th International Polymers for Advanced Technologies Conference, PAT 2013. Berlin (D), 2013.}} @misc{rijckaert_evaluation_of_2013, author={Rijckaert, B., Pierce, B.F., Neffe, A.T., Goers, J., Roch, T., Gebauer, T., Smink, J.J., Lendlein, A., Gossen, M., Leutz, A.}, title={Evaluation of Gelatin Hydrogels in their Interaction with Monocytic Osteoclast Precursor Cells}, year={2013}, howpublished = {conference poster: Berlin (D);}, note = {Rijckaert, B.; Pierce, B.; Neffe, A.; Goers, J.; Roch, T.; Gebauer, T.; Smink, J.; Lendlein, A.; Gossen, M.; Leutz, A.: Evaluation of Gelatin Hydrogels in their Interaction with Monocytic Osteoclast Precursor Cells. In: 12th International Conference on Polymers for Advanced Technologies, PAT 2013. Berlin (D). 2013.}} @misc{julichgruner_functionalization_of_2013, author={Julich-Gruner, K., Roch, T., Neffe, A.T., Lendlein, A.}, title={Functionalization of Polymers with the Amino Acid derived Moieties Desaminotyrosine and Desaminotyrosyl Tyrosine}, year={2013}, howpublished = {conference poster: Berlin (D);}, note = {Julich-Gruner, K.; Roch, T.; Neffe, A.; Lendlein, A.: Functionalization of Polymers with the Amino Acid derived Moieties Desaminotyrosine and Desaminotyrosyl Tyrosine. In: 12th International Conference on Polymers for Advanced Technologies, PAT 2013. Berlin (D). 2013.}} @misc{luetzow_shapememory_effect_2013, author={Luetzow, K., Weigel, T., Kosmella, H.-J., Neffe, A.T., Lendlein, A.}, title={Shape-Memory Effect of Polymeric Nanocomposite Polyether Urethane Foams with Magnetic Nanoparticles}, year={2013}, howpublished = {conference poster: Berlin (D);}, note = {Luetzow, K.; Weigel, T.; Kosmella, H.; Neffe, A.; Lendlein, A.: Shape-Memory Effect of Polymeric Nanocomposite Polyether Urethane Foams with Magnetic Nanoparticles. In: 12th International Conference on Polymers for Advanced Technologies, PAT 2013. Berlin (D). 2013.}} @misc{neffe_biopolymerbased_materials_2012, author={Neffe, A.T., Behl, M., Jung, F., Pierce, B.F., Lendlein, A.}, title={Biopolymer-Based Materials for Medical Applications}, year={2012}, howpublished = {conference lecture (invited): Adelaide (AUS);}, note = {Neffe, A.; Behl, M.; Jung, F.; Pierce, B.; Lendlein, A.: Biopolymer-Based Materials for Medical Applications. Smart Collaborations Seminar. Adelaide (AUS), 2012.}} @misc{neffe_biomimetic_polymers_2012, author={Neffe, A.T., Pierce, B.F., Jung, F., Ma, N., Lendlein, A.}, title={Biomimetic Polymers for Biomedical Applications}, year={2012}, howpublished = {conference lecture (invited): Adelaide (AUS);}, note = {Neffe, A.; Pierce, B.; Jung, F.; Ma, N.; Lendlein, A.: Biomimetic Polymers for Biomedical Applications. Chemistry Seminar University of Adelaide. Adelaide (AUS), 2012.}} @misc{neffe_biomimetic_polymers_2012, author={Neffe, A.T., Pierce, B.F., Jung, F., Ma, N., Lendlein, A.}, title={Biomimetic Polymers for Biomedical Applications}, year={2012}, howpublished = {conference lecture (invited): Adelaide (AUS);}, note = {Neffe, A.; Pierce, B.; Jung, F.; Ma, N.; Lendlein, A.: Biomimetic Polymers for Biomedical Applications. Chemistry Seminar Flinders University. Adelaide (AUS), 2012.}} @misc{julichgruner_synthesis_and_2012, author={Julich-Gruner, K.K., Neffe, A.T., Lendlein, A.}, title={Synthesis and characterization of oligo(ethylene glycol)s functionalized with desaminotyrosine or desaminotyrosyltyrosine}, year={2012}, howpublished = {conference poster: Berlin (D);}, note = {Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Synthesis and characterization of oligo(ethylene glycol)s functionalized with desaminotyrosine or desaminotyrosyltyrosine. In: Polydays 2012. Berlin (D). 2012.}} @misc{neffe_biomaterialien_ein_2012, author={Neffe, A.T., Lendlein, A.}, title={Biomaterialien: Ein Gewinn fuer die Regenerative Medizin und den Gesundheitsstandort Berlin-Brandenburg}, year={2012}, howpublished = {journal article}, abstract = {Biomaterialien sind Bestandteil regenerativer Therapien. Durch Vernetzung von herausragender Grundlagenforschung, klinischer Forschung mit Anwendungsorientierter Forschung und Entwicklung sowie einer interdisziplinären Ausbildung wird die gesamte Entwicklungskaskade abgebildet, so dass die Biomaterialforschung in der Region Berlin-Brandenburg einen internationalen Spitzenplatz belegt.}, note = {Neffe, A.; Lendlein, A.: Biomaterialien: Ein Gewinn fuer die Regenerative Medizin und den Gesundheitsstandort Berlin-Brandenburg. Berliner Wirtschaftsgespraeche, Themenbroschuere 2012, Gesundheitsstandort Berlin und Brandenburg. 2012. 73-74.}} @misc{goers_influence_of_2012, author={Goers, J., Mayer, A., Zaupa, A., Pierce, B., Neffe, A.T., Lendlein, A., Jung, F.}, title={Influence of physically (DAT) crosslinked gelatin on the vasculature in the avian chorioallantoic membrane}, year={2012}, howpublished = {conference poster: Halle / Saale (D);}, note = {Goers, J.; Mayer, A.; Zaupa, A.; Pierce, B.; Neffe, A.; Lendlein, A.; Jung, F.: Influence of physically (DAT) crosslinked gelatin on the vasculature in the avian chorioallantoic membrane. In: 31. Jahrestagung der Deutschen Gesellschaft fuer klinische Mikrozirkulation und Haemorheologie. Halle / Saale (D). 2012.}} @misc{julichgruner_synthesis_and_2012, author={Julich-Gruner, K.K., Neffe, A.T., Lendlein, A.}, title={Synthesis and characterization of oligo(ethylene glycol)s functionalized with desaminotyrosine or desaminotyrosyltyrosine}, year={2012}, howpublished = {conference poster: Vico Equense (I);}, note = {Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Synthesis and characterization of oligo(ethylene glycol)s functionalized with desaminotyrosine or desaminotyrosyltyrosine. In: Advanced Functional Polymers for Medicine, AFPM 2012. Vico Equense (I). 2012.}} @misc{neffe_biomaterials_for_2012, author={Neffe, A.T., Lendlein, A.}, title={Biomaterials for Regenerative Medicine}, year={2012}, howpublished = {conference lecture (invited): Bruessel (B);}, note = {Neffe, A.; Lendlein, A.: Biomaterials for Regenerative Medicine. Improving Health for the Ageing Society, Dialogue Forum. Bruessel (B), 2012.}} @misc{goers_influence_of_2012, author={Goers, J., Krueger, A., Gebauer, T., Pierce, B.F., Neffe, A.T., Lendlein, A., Jung, F.}, title={Influence of chemically crosslinked gelatin on the vasculature in the avian chorioallantoic membrane}, year={2012}, howpublished = {conference lecture: Istanbul (TR);}, note = {Goers, J.; Krueger, A.; Gebauer, T.; Pierce, B.; Neffe, A.; Lendlein, A.; Jung, F.: Influence of chemically crosslinked gelatin on the vasculature in the avian chorioallantoic membrane. 14th International Congress of Biorheology, 7th International Conference on Clinical Hemorheology. Istanbul (TR), 2012.}} @misc{neffe_biomaterials_in_2012, author={Neffe, A.T., Scharnagl, N., Behl, M., Lendlein, A.}, title={Biomaterials in Regenerative Medicine}, year={2012}, howpublished = {journal article}, abstract = {meets application-motivated science aiming at translation of the gained knowledge into products and clinical applications.}, note = {Neffe, A.; Scharnagl, N.; Behl, M.; Lendlein, A.: Biomaterials in Regenerative Medicine. BioTOPics : Journal of Biotechnology in Berlin-Brandenburg. 2012. vol. 43, 8-10.}} @misc{neffe_zukunftsperspektiven_in_2012, author={Neffe, A.T., Lendlein, A.}, title={Zukunftsperspektiven in der Biomaterialforschung}, year={2012}, howpublished = {conference lecture (invited): Nuernberg (D);}, note = {Neffe, A.; Lendlein, A.: Zukunftsperspektiven in der Biomaterialforschung. Medi-WING, Medizintechnik im BMBF-Foerderprogramm WING. Nuernberg (D), 2012.}} @misc{pierce_photocrosslinked_conetworks_2012, author={Pierce, B.F., Tronci, G., Roessle, M., Neffe, A.T., Jung, F., Lendlein, A.}, title={Photocrosslinked Co-Networks from Glycidylmethacrylated Gelatin and Poly(ethylene glycol) Methacrylates}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.1002/mabi.201100232}, abstract = {Biopolymer-based systems with adjustable macroscopic properties that can be varied in a wide range using only small changes in chemical composition are promising candidates for biomaterial-induced autoregeneration. Glycidylmethacrylated gelatin is photopolymerized with the addition of PEG mono- or dimethacrylate to form co-networks in pH = 7.4 PBS. The degree of swelling (Q) and water uptake (H) in PBS at 37 °C are tailorable for PEGDMA co-networks (Q ≈ 250–650 vol%), while the storage modulus of swollen networks at 37 °C can be adjusted by the PEG(D)MA content (G′ = 0.7–145 kPa). Indirect cytotoxicity tests on ethylene oxide sterilized films show non-toxic responses for the homonetwork and all but one PEGDMA-containing co-networks materials.}, note = {Online available at: \url{https://doi.org/10.1002/mabi.201100232} (DOI). Pierce, B.; Tronci, G.; Roessle, M.; Neffe, A.; Jung, F.; Lendlein, A.: Photocrosslinked Co-Networks from Glycidylmethacrylated Gelatin and Poly(ethylene glycol) Methacrylates. Macromolecular Bioscience. 2012. vol. 12, no. 4, 484-493. DOI: 10.1002/mabi.201100232}} @misc{neffe_surface_functionalization_2012, author={Neffe, A.T., Lange, M., Braune, S., Luetzow, K., Jung, F., Scharnagl, N., Richau, K., Weinhart, M., Haag, R., Lendlein, A.}, title={Surface Functionalization of Poly(ether imide) with Linear, Methylated Oligoglycerols to Increase the Hemocompatibility}, year={2012}, howpublished = {conference poster: Vico Equense (I);}, note = {Neffe, A.; Lange, M.; Braune, S.; Luetzow, K.; Jung, F.; Scharnagl, N.; Richau, K.; Weinhart, M.; Haag, R.; Lendlein, A.: Surface Functionalization of Poly(ether imide) with Linear, Methylated Oligoglycerols to Increase the Hemocompatibility. In: Advanced Functional Polymers for Medicine, AFPM 2012. Vico Equense (I). 2012.}} @misc{jung_physically_crosslinked_2012, author={Jung, F., Goers, J., Roch, T., Zaupa, A., Pierce, B., Neffe, A.T., Lendlein, A.}, title={Physically crosslinked gelatins functionalized with tyrosine moieties do not induce angiogenesis or thrombus formation in the developing vasculature in the avian chorioallantoic membrane}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.3233/CH-2011-1443}, abstract = {Gelatins functionalized with desaminotyrosine or desaminotyrosyl tyrosine form physically crosslinked polymer networks due to the interactions between the introduced aromatic moeties. In the swollen state, their mechanical properties can be tailored in a range similar to the elasticity of soft tissues. The aim of this study was to evaluate their potential as biomaterials by determining whether these materials – in comparison to plain gelatin – induce bleedings, thrombotic processes, or angiogenesis. These investigations were performed using the hen's egg chorioallantoic membrane (HETCAM) assay. These results indicate that the gelatin-based hydrogels did not possess angiogenic effects and also did not induce bleedings, thrombotic processes or vessel destruction (avascular zones). The biocompatibility of the materials in vitro motivates the exploration of their application as matrix in local drug-release systems with short half-life times (1 hour up to several days).}, note = {Online available at: \url{https://doi.org/10.3233/CH-2011-1443} (DOI). Jung, F.; Goers, J.; Roch, T.; Zaupa, A.; Pierce, B.; Neffe, A.; Lendlein, A.: Physically crosslinked gelatins functionalized with tyrosine moieties do not induce angiogenesis or thrombus formation in the developing vasculature in the avian chorioallantoic membrane. Clinical Hemorheology and Microcirculation. 2012. vol. 50, no. 1-2, 55-63. DOI: 10.3233/CH-2011-1443}} @misc{neffe_biomaterials_for_2012, author={Neffe, A.T., Lendlein, A.}, title={Biomaterials for Regenerative Medicine}, year={2012}, howpublished = {conference lecture: Berlin (D);}, note = {Neffe, A.; Lendlein, A.: Biomaterials for Regenerative Medicine. TERM - Trends and challenges in Regenerative Medicine - Towards interregional collaboration in Europe. Berlin (D), 2012.}} @misc{lange_characterization_of_2012, author={Lange, M., Luetzow, K., Neffe, A.T., Lendlein, A.}, title={Characterization of Oligo(ethylene glycol) and Oligoglycerol functionalized Poly(ether imide) by Angle-dependent X-ray Photoelectron Spectroscopy}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.5301/JABFM.2012.10345}, abstract = {Purpose: Previous investigations have shown that poly(ether imide) (PEI) membranes can be functionalized with aminated macromolecules. In this study we explored whether the characterization of PEI functionalized with oligo(ethylene glycol) (OEG) or linear, side chain methylated oligoglycerols (OGMe), by angle-dependent X-ray induced photoelectron spectroscopy (XPS) can be used to prove the functionalization, give insight into the reaction mechanism and reveal the spatial distribution of the grafts. 
Methods: PEI membranes were functionalized under alkaline conditions using an aqueous solution with 2 wt% of α-amino-ω-methoxy oligo(ethylene glycol) (Mn = 1,320 g·mol-1) or linear, side chain methylated monoamine oligoglycerols (Mn = 1,120, 1,800 or 2,270 g·mol-1), respectively. The functionalized membranes were investigated using XPS measurements at different detector angles to enable comparison between the signals related to the bulk and surface volume and were compared with untreated and alkaline-treated PEI membranes.
 Results: While at a perpendicular detector angle the bulk signals of the PEI were prominent, at larger surface volume-related detector angles, the signals for OGMe and OEG were determinable. 
Conclusion: The surface functionalization of PEI with OEG and OGMe could be verified by the angle-dependent XPS. The observations proved the functionalization at the PEI surface, as the polyethers were detected at angles providing signals of the surface volume. Furthermore, the chemical functions determined verified a covalent binding via the nucleophilic addition of the amine functionalized OGMe and OEG to the PEI imide function.}, note = {Online available at: \url{https://doi.org/10.5301/JABFM.2012.10345} (DOI). Lange, M.; Luetzow, K.; Neffe, A.; Lendlein, A.: Characterization of Oligo(ethylene glycol) and Oligoglycerol functionalized Poly(ether imide) by Angle-dependent X-ray Photoelectron Spectroscopy. Journal of Applied Biomaterials & Functional Materials. 2012. vol. 10, no. 3, 215-222. DOI: 10.5301/JABFM.2012.10345}} @misc{neffe_control_of_2012, author={Neffe, A.T., Pierce, B.F., Lendlein, A.}, title={Control of Mechanical Properties in Biopolymer-Based Biomaterials}, year={2012}, howpublished = {conference lecture: Rostock (D);}, note = {Neffe, A.; Pierce, B.; Lendlein, A.: Control of Mechanical Properties in Biopolymer-Based Biomaterials. 39th Congress of the European Society for Artificial Organs, ESAO 2012. Rostock (D), 2012.}} @misc{santan_thermal_gelation_2012, author={Santan, H.D., Neffe, A.T., Kamlage, S., Lendlein, A.}, title={Thermal Gelation and Stability of Pectin Grafted with PEPE}, year={2012}, howpublished = {conference paper: Boston, MA (USA);}, note = {Santan, H.; Neffe, A.; Kamlage, S.; Lendlein, A.: Thermal Gelation and Stability of Pectin Grafted with PEPE. In: Lendlein, A.; Behl, M.; Feng, Y.; Guan, Z.; Xie, T. (Ed.): MRS Symposium Proceedings, Multifunctional Polymer-Based Materials, 2011 MRS Fall Meeting. Boston, MA (USA). Cambridge University Press. 2012. 151-158.}} @misc{neffe_quantifying_protein_2012, author={Neffe, A.T., Pierce, B.F., Blaszkiewicz, J., Lendlein, A.}, title={Quantifying Protein Adsorption to Physically Crosslinked Gelatin-Based Networks}, year={2012}, howpublished = {conference paper: Boston, MA (USA);}, note = {Neffe, A.; Pierce, B.; Blaszkiewicz, J.; Lendlein, A.: Quantifying Protein Adsorption to Physically Crosslinked Gelatin-Based Networks. In: Lendlein, A.; Behl, M.; Feng, Y.; Guan, Z.; Xie, T. (Ed.): MRS Symposium Proceedings, Multifunctional Polymer-Based Materials, 2011 MRS Fall Meeting. Boston, MA (USA). Cambridge University Press. 2012. 145-150.}} @misc{piece_using_mass_2012, author={Piece, B.F., Neffe, A.T., Lendlein, A.}, title={Using Mass Spectrometry to Investigate the Structural Features of Photocrosslinked Co-Networks based on Gelatin and Poly(ethylene glycol) Methacrylates}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2012.221}, abstract = {Gelatin was functionalized with glycidyl methacrylate and photocrosslinked in the presence of poly(ethylene glycol) dimethacrylate (PEGDMA) or poly(ethylene glycol) monomethacrylate (PEGMA) to create a biopolymer-based system with tailorable properties. These co-networks were hydrolyzed using 6 M HCl and the degradation products were analyzed and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. This technique successfully identified gelatin-derived peptides such as FLPEPPE, SFLPEPPE, and SFLPEPPEE as well as an accompanying PEG-g-poly(methacrylic acid) component. No oligo- or polymethacrylates were monitored at any molecular weight range above m/z = 500, which indicated that they possessed lower molecular weights. An in vitro hydrolytic degradation experiment performed in pH 7.4 PBS buffer solution at 37 °C showed that these networks, which were prepared without the addition of a potentially toxic photoinitiator, exhibited mass loss of up to 50 wt% at 6 weeks of incubation time. These results provide valuable insight into how these functional gelatin-based co-network biomaterials will perform in a biological setting.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2012.221} (DOI). Piece, B.; Neffe, A.; Lendlein, A.: Using Mass Spectrometry to Investigate the Structural Features of Photocrosslinked Co-Networks based on Gelatin and Poly(ethylene glycol) Methacrylates. MRS Online Proceedings Library. 2012. vol. 1403, 13-18. DOI: 10.1557/opl.2012.221}} @misc{piece_using_mass_2012, author={Piece, B.F., Neffe, A.T., Lendlein, A.}, title={Using Mass Spectrometry to Investigate the Structural Features of Photocrosslinked Co-Networks based on Gelatin and Poly(ethylene glycol) Methacrylates}, year={2012}, howpublished = {conference paper: Boston, MA (USA);}, note = {Piece, B.; Neffe, A.; Lendlein, A.: Using Mass Spectrometry to Investigate the Structural Features of Photocrosslinked Co-Networks based on Gelatin and Poly(ethylene glycol) Methacrylates. In: Lendlein, A.; Behl, M.; Feng, Y.; Guan, Z.; Xie, T. (Ed.): MRS Symposium Proceedings, Multifunctional Polymer-Based Materials, 2011 MRS Fall Meeting. Boston, MA (USA). Cambridge University Press. 2012. 159-165.}} @misc{lendlein_knowledgebased_design_2012, author={Lendlein, A., Pierce, B.F., Neffe, A.T.}, title={Knowledge-based design of degradable polymers}, year={2012}, howpublished = {conference lecture (invited): Chengdu (VRC);}, note = {Lendlein, A.; Pierce, B.; Neffe, A.: Knowledge-based design of degradable polymers. 9th World Biomaterials Congress. Chengdu (VRC), 2012.}} @misc{pierce_viability_of_2012, author={Pierce, B.F., Pittermann, E., Ma, N., Gebauer, T., Neffe, A.T., Hoelscher, M., Jung, F., Lendlein, A.}, title={Viability of Human Mesenchymal Stem Cells Seeded on Crosslinked Entropy-Elastic Gelatin-Based Hydrogels}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.1002/mabi.201100237}, abstract = {Biomimetic polymer network systems with tailorable properties based on biopolymers represent a class of degradable hydrogels that provides sequences for protein adsorption and cell adhesion. Such materials show potential for in vitro MSC proliferation as well as in vivo applications and were obtained by crosslinking different concentrations of gelatin using varying amounts of ethyl lysine diisocyanate in the presence of a surfactant in pH 7.4 PBS solution. Material extracts, which were tested for cytotoxic effects using L929 mouse fibroblasts, were non-toxic. The hydrogels were seeded with human bone marrow-derived MSCs and supported viable MSCs for the incubation time of 9 d. Preadsorption of fibronectin on materials improved this biofunctionality.}, note = {Online available at: \url{https://doi.org/10.1002/mabi.201100237} (DOI). Pierce, B.; Pittermann, E.; Ma, N.; Gebauer, T.; Neffe, A.; Hoelscher, M.; Jung, F.; Lendlein, A.: Viability of Human Mesenchymal Stem Cells Seeded on Crosslinked Entropy-Elastic Gelatin-Based Hydrogels. Macromolecular Bioscience. 2012. vol. 12, no. 3, 312-321. DOI: 10.1002/mabi.201100237}} @misc{lange_characterization_of_2012, author={Lange, M., Luetzow, K., Neffe, A.T., Lendlein, A.}, title={Characterization of Oligo(ethylene glycol) and Oligoglycerol functionalized Poly(ether imide) by Angle-dependent X-ray Photoelectron Spectroscopy}, year={2012}, howpublished = {conference lecture: Vico Equense (I);}, note = {Lange, M.; Luetzow, K.; Neffe, A.; Lendlein, A.: Characterization of Oligo(ethylene glycol) and Oligoglycerol functionalized Poly(ether imide) by Angle-dependent X-ray Photoelectron Spectroscopy. Advanced Functional Polymers for Medicine, AFPM 2012. Vico Equense (I), 2012.}} @misc{julichgruner_synthesis_and_2012, author={Julich-Gruner, K.K., Neffe, A.T., Lendlein, A.}, title={Synthesis and characterization of oligo(ethylene glycol)s functionalized with desaminotyrosine or desaminotyrosyltyrosine}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.5301/JABFM.2012.10342}, abstract = {Purpose: The aromatic compounds desaminotyrosine (DAT) and desaminotyrosyltyrosine (DATT) have been successfully used to functionalize gelatin in order to form physically crosslinked networks via π-π interactions and hydrogen bonds of the introduced phenol moieties. Here, it was explored whether this concept can be applied to a synthetic polymer not engaging in additional interactions such as triple helix formation in gelatin, enabling a network to form by physical interactions mainly related to the terminal functional groups. Oligo(ethylene glycol) (OEG) was chosen as hydrophilic synthetic polymer for the backbone structure. Methods: Linear OEG (MP = 3 kDa) and four-arm OEG (Mn = 5 kDa) with amino functionalities as endgroups were functionalized with DAT and DATT using EDC·HCl and NHS as activating agents. The compounds were characterized by NMR, IR spectroscopy, and MALDI. Rheological behavior of aqueous solutions of the polymers was studied. The critical micelle concentration (CMC) was determined by a fluorescence spectroscopic analysis using the hydrophobic fluorescent dye pyrene. Results: DATT-functionalized linear OEG, four-arm DAT-functionalized OEG and four-arm DATT-functionalized OEG were synthesized with degrees of functionalization of 60-95 mol%. All compounds were water soluble, and rheological measurements revealed a decrease in storage modulus G’ and loss modulus G’’ compared to unfunctionalized OEG. Moreover, the CMC of linear OEG-DATT could be determined. Conclusions: The syntheses of OEG functionalized with the aromatic compounds DAT and DATT was reported. The polymers showed the properties of a surfactant.}, note = {Online available at: \url{https://doi.org/10.5301/JABFM.2012.10342} (DOI). Julich-Gruner, K.; Neffe, A.; Lendlein, A.: Synthesis and characterization of oligo(ethylene glycol)s functionalized with desaminotyrosine or desaminotyrosyltyrosine. Journal of Applied Biomaterials & Functional Materials. 2012. vol. 10, no. 3, 170-176. DOI: 10.5301/JABFM.2012.10342}} @misc{neffe_thermal_gelation_2012, author={Neffe, A.T., Santan, H.D., Kamlage, S., Lendlein, A.}, title={Thermal Gelation and Stability of Pectin and Chondroitin Sulfate Grafted with Poly(ethylene glycol-b-propylene glycol-b-ethylene glycol)}, year={2012}, howpublished = {conference poster: Mainz (D);}, note = {Neffe, A.; Santan, H.; Kamlage, S.; Lendlein, A.: Thermal Gelation and Stability of Pectin and Chondroitin Sulfate Grafted with Poly(ethylene glycol-b-propylene glycol-b-ethylene glycol). In: GDCh Biannual Meeting of the GDCh-Division Macromolecular Chemistry, Smart Polymers. Mainz (D). 2012.}} @misc{lange_surface_functionalization_2012, author={Lange, M., Braune, S., Luetzow, K., Richau, K., Scharnagl, N., Weinhart, M., Neffe, A.T., Jung, F., Haag, R., Lendlein, A.}, title={Surface Functionalization of Poly(ether imide) Membranes with Linear, Methylated Oligoglycerols for Reducing Thrombogenicity}, year={2012}, howpublished = {journal article}, doi = {https://doi.org/10.1002/marc.201200426}, abstract = {Materials for biomedical applications are often chosen for their bulk properties. Other requirements such as a hemocompatible surface shall be fulfilled by suitable chemical functionalization. Here we show, that linear, side-chain methylated oligoglycerols (OGMe) are more stable to oxidation than oligo(ethylene glycol) (OEG). Poly(ether imide) (PEI) membranes functionalized with OGMes perform at least as good as, and partially better than, OEG functionalized PEI membranes in view of protein resistance as well as thrombocyte adhesion and activation. Therefore, OGMes are highly potent surface functionalizing molecules for improving the hemocompatibility of polymers.}, note = {Online available at: \url{https://doi.org/10.1002/marc.201200426} (DOI). Lange, M.; Braune, S.; Luetzow, K.; Richau, K.; Scharnagl, N.; Weinhart, M.; Neffe, A.; Jung, F.; Haag, R.; Lendlein, A.: Surface Functionalization of Poly(ether imide) Membranes with Linear, Methylated Oligoglycerols for Reducing Thrombogenicity. Macromolecular Rapid Communications. 2012. vol. 33, no. 17, 1487-1492. DOI: 10.1002/marc.201200426}} @misc{wischke_sustained_release_2012, author={Wischke, C., Schneider, C., Neffe, A.T., Lendlein, A.}, title={Sustained release hydrogels by in situ polymerized coating with polyalkylcyanoacrylates}, year={2012}, howpublished = {conference poster: Souzhou (VRC);}, note = {Wischke, C.; Schneider, C.; Neffe, A.; Lendlein, A.: Sustained release hydrogels by in situ polymerized coating with polyalkylcyanoacrylates. In: 2nd Symposium on Innovative Polymers for Controlled Delivery, SIPCD 2012. Souzhou (VRC). 2012.}} @misc{neffe_functionalization_of_2012, author={Neffe, A.T., Zaupa, A., Pierce, B.F., Roch, T., Jung, F., Lendlein, A.}, title={Functionalization of Gelatin with Tyrosine Moieties: A Supramolecular Approach to Biomaterials}, year={2012}, howpublished = {conference poster: Wien (A);}, note = {Neffe, A.; Zaupa, A.; Pierce, B.; Roch, T.; Jung, F.; Lendlein, A.: Functionalization of Gelatin with Tyrosine Moieties: A Supramolecular Approach to Biomaterials. In: COST Namabio 2nd Joint Meeting. Wien (A). 2012.}} @misc{neffe_polymere__2011, author={Neffe, A.T., Behl, M., Lendlein, A.}, title={Polymere}, year={2011}, howpublished = {report part}, note = {Neffe, A.; Behl, M.; Lendlein, A.: Polymere. In: BioTOP Berlin-Brandenburg (Ed.): Technologiereport - Regenerative Medizin in Berlin-Brandenburg. TSB Innovationsagentur Berlin GmbH. 2011. 73-75.}} @misc{tripodo_highly_elastic_2011, author={Tripodo, G., Wischke, C., Neffe, A.T., Lendlein, A.}, title={Highly elastic poly(ethyl-2-cyanoacrylate) based materials obtained by incorporation of oligo(ethylene glycol) diglycidyl ether as a softener}, year={2011}, howpublished = {conference poster: Enschede (NL);}, note = {Tripodo, G.; Wischke, C.; Neffe, A.; Lendlein, A.: Highly elastic poly(ethyl-2-cyanoacrylate) based materials obtained by incorporation of oligo(ethylene glycol) diglycidyl ether as a softener. In: Advanced Functional Polymers for Medicine, AFPM 2011. Enschede (NL). 2011.}} @misc{neffe_gelatine_mit_2011, author={Neffe, A.T., Zaupa, A., Lendlein, A.}, title={Gelatine mit definierten physikalischen Netzpunkten als Basis fuer supramolekulare, multifunktionale Biomaterialien}, year={2011}, howpublished = {conference lecture: Karlsruhe (D);}, note = {Neffe, A.; Zaupa, A.; Lendlein, A.: Gelatine mit definierten physikalischen Netzpunkten als Basis fuer supramolekulare, multifunktionale Biomaterialien. Bioorganik Symposium, Nachwuchsgruppenleitertreffen. Karlsruhe (D), 2011.}} @misc{neffe_quantifying_protein_2011, author={Neffe, A.T., Pierce, B.F., Blaszkiewicz, J., Lendlein, A.}, title={Quantifying Protein Adsorption to Physically Crosslinked Gelatin-Based Networks}, year={2011}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Neffe, A.; Pierce, B.; Blaszkiewicz, J.; Lendlein, A.: Quantifying Protein Adsorption to Physically Crosslinked Gelatin-Based Networks. MRS Fall 2011 Meeting, Symposium V. Boston, MA (USA), 2011.}} @misc{jung_hemocompatibility_testing_2011, author={Jung, F., Neffe, A.T., Lendlein, A.}, title={Hemocompatibility Testing of Polymers and Hemo-/Histocompatible Polymers}, year={2011}, howpublished = {conference lecture: Porto (P);}, note = {Jung, F.; Neffe, A.; Lendlein, A.: Hemocompatibility Testing of Polymers and Hemo-/Histocompatible Polymers. 38th Congress of the European Society for Artificial Organs, ESAO 2011. Porto (P), 2011.}} @misc{lendlein_shape_memory_2011, author={Lendlein, A., Wischke, C., Kratz, K., Heuchel, M., Zotzmann, J., Hiebl, B., Neffe, A., Behl, M.}, title={Shape- Memory Polymers}, year={2011}, howpublished = {book part}, abstract = {Medical devices such as implants, surgical instruments, extracorporal devices or wound covers, as well as controlled drug delivery systems (CDDS) require a specific combination of material properties and functions including, e.g., mechanical stability, biocompatibility, or biofunctionality. Polymeric biomaterials are of high relevance for such applications, as properties and functions can be tuned in a wide range by only small defined variations of their chemical or morphological structure. The rapid progress in surgical techniques, especially in minimally-invasive surgery, requires smart materials, which are capable of an active on-demand movement and which do not need to be removed in a second surgery. These challenges can be addressed by shape-memory polymers (SMPs) described in this chapter. SMPs are of high technological significance for biomedical applications as they enable on demand predefined changes in the shape of a device upon exposure to a suitable stimulus. Multifunctional materials are obtained when the shape-memory effect is combined with an additional function such as hydrolytic degradability, biofunctionality, and controlled drug release. Selected biomaterials with shape-memory capability are presented, including data on their biocompatibility. The potential of SMPs as a platform technology for biomedical applications is sketched by an overview on SMP-based medical devices being developed and the potential use of SMPs as matrix for CDDS.}, note = {Lendlein, A.; Wischke, C.; Kratz, K.; Heuchel, M.; Zotzmann, J.; Hiebl, B.; Neffe, A.; Behl, M.: Shape- Memory Polymers. In: Ducheyne, P.; Healy, K.; Hutmacher, D.; Grainger, D.; Kirkpatrick, C. (Ed.): Comprehensive Biomaterials. Elsevier. 2011. 479-496.}} @misc{kobuch_in_vivo_2011, author={Kobuch, K.A., Maier, M., Feucht, N., Wolfstein, A., Streufert, D., Lohmann, C.P., Kamlage, S., Neffe, A.T., Lendlein, A.}, title={In vivo Evaluation of a Multifunctional Hydrogel System for Local Application on the Retina}, year={2011}, howpublished = {conference poster: Twente (NL);}, note = {Kobuch, K.; Maier, M.; Feucht, N.; Wolfstein, A.; Streufert, D.; Lohmann, C.; Kamlage, S.; Neffe, A.; Lendlein, A.: In vivo Evaluation of a Multifunctional Hydrogel System for Local Application on the Retina. In: Advanced Functional Polymers for Medicine, AFPM 2011. Twente (NL). 2011.}} @misc{roch_analysis_of_2011, author={Roch, T., Pierce, B.F., Zaupa, A., Goers, J., Jung, F., Neffe, A.T., Lendlein, A.}, title={Analysis of Endotoxin Content and Evaluation of Angiogenic Effects of Desaminotyrosine- and Desaminotyrosyl Tyrosine-Functionalized Gelatin}, year={2011}, howpublished = {conference poster: Twente (NL);}, note = {Roch, T.; Pierce, B.; Zaupa, A.; Goers, J.; Jung, F.; Neffe, A.; Lendlein, A.: Analysis of Endotoxin Content and Evaluation of Angiogenic Effects of Desaminotyrosine- and Desaminotyrosyl Tyrosine-Functionalized Gelatin. In: Advanced Functional Polymers for Medicine, AFPM 2011. Twente (NL). 2011.}} @misc{lendlein_why_are_2011, author={Lendlein, A., Neffe, A.T., Pierce, B., Vienken, J.}, title={Why are so few degradable polymeric biomaterials currently established in clinical applications?}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.5301/IJAO.2011.6422}, abstract = {No abstract}, note = {Online available at: \url{https://doi.org/10.5301/IJAO.2011.6422} (DOI). Lendlein, A.; Neffe, A.; Pierce, B.; Vienken, J.: Why are so few degradable polymeric biomaterials currently established in clinical applications?. The International Journal of Artificial Organs. 2011. vol. 34, no. 2, 71-75. DOI: 10.5301/IJAO.2011.6422}} @misc{lendlein_shapememory_polymers_2011, author={Lendlein, A., Neffe, A., Wischke, C.}, title={Shape-memory Polymers as Platform Technology for Multifunctional Materials}, year={2011}, howpublished = {conference lecture (invited): Shenzhen (VRC);}, note = {Lendlein, A.; Neffe, A.; Wischke, C.: Shape-memory Polymers as Platform Technology for Multifunctional Materials. 3rd International Conference on Smart Materials and Nanotechnology in Engineering, SMN 2011. Shenzhen (VRC), 2011.}} @misc{neffe_in_vitro_2011, author={Neffe, A.T., Kobuch, K.A., Maier, M., Feucht, N., Lohmann, C.P., Wolfstein, A., Streufert, D., Kamlage, S., Lendlein, A.}, title={In vitro and in vivo Evaluation of a Multifunctional Hyaluronic acid based Hydrogel System for Local Application on the Retina}, year={2011}, howpublished = {conference poster: Twente (NL);}, note = {Neffe, A.; Kobuch, K.; Maier, M.; Feucht, N.; Lohmann, C.; Wolfstein, A.; Streufert, D.; Kamlage, S.; Lendlein, A.: In vitro and in vivo Evaluation of a Multifunctional Hyaluronic acid based Hydrogel System for Local Application on the Retina. In: Advanced Functional Polymers for Medicine, AFPM 2011. Twente (NL). 2011.}} @misc{loebler_polymers_and_2011, author={Loebler, M., Sternberg, K., Stachs, O., Allemann, R., Grabow, N., Roock, A., Kreiner, C.F., Streufert, D., Neffe, A.T., Hanh, B.D., Lendlein, A., Schmitz, K.-P., Guthoff, R.}, title={Polymers and drugs suitable for the development of a drug delivery drainage system in glaucoma surgery}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/jbm.b.31826}, abstract = {Implantation of a glaucoma drainage system is an appropriate therapeutic intervention in some glaucoma patients. However, one drawback with this approach is the fibrotic tissue response to the implant material, leading to reduced flow of aqueous liquid or complete blockage of the drainage system. As a basis for developing an aqueous shunt we report here investigations with poly(3-hydroxybutyrate) (P(3HB)) and poly(4-hydroxybutyrate) (P(4HB)) as polymer matrices and with paclitaxel (PTX) and triamcinolone acetonide (TA) as drugs that might, in combination, delay or prevent the process of fibrosis by reducing fibroblast activity. P(3HB) and P(4HB) were fabricated into test prototypes with 500 μm outer and 200 μm inner diameter and ∼1 cm length. The antiproliferative agent PTX and the anti-inflammatory agent TA were incorporated into the polymer matrices and were released by diffusion. In vitro cell assays demonstrated that the polymers have the potential to reduce fibroblast viability, while TA showed differential inhibition of Tenon fibroblasts, but not cornea keratocytes. Implantation of polymer disks and prototype devices into rabbit eyes confirmed the good biocompatibility of the materials. The combined use of a poly(hydroxybutyrate) polymer with PTX or TA has the potential to reduce the fibrosis associated with conventional glaucoma drainage systems.}, note = {Online available at: \url{https://doi.org/10.1002/jbm.b.31826} (DOI). Loebler, M.; Sternberg, K.; Stachs, O.; Allemann, R.; Grabow, N.; Roock, A.; Kreiner, C.; Streufert, D.; Neffe, A.; Hanh, B.; Lendlein, A.; Schmitz, K.; Guthoff, R.: Polymers and drugs suitable for the development of a drug delivery drainage system in glaucoma surgery. Journal of Biomedical Materials Research B. 2011. vol. 97, no. 2, 388-395. DOI: 10.1002/jbm.b.31826}} @misc{neffe_gelatin_functionalization_2011, author={Neffe, A.T., Loebus, A., Zaupa, A., Stoetzel, C., Mueller, F.A., Lendlein, A.}, title={Gelatin Functionalization with Tyrosine Derived Moieties for Increasing the Interaction with Hydroxyapatite Fillers}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.actbio.2010.11.025}, abstract = {Combining gelatins functionalized with the tyrosine-derived groups desaminotyrosine or desaminotyrosyl tyrosine with hydroxyapatite (HAp) led to the formation of composite materials with much lower swelling ratio than of the pure matrices. Shifts of IR bands related to the free carboxyl groups could be observed in the presence of HAp, which suggested a direct interaction of matrix and filler that formed additional physical crosslinks in the material. In tensile tests and rheological measurements, the composites equilibrated in water had increased Young’s moduli (from 200 kPa up to 2 MPa) and tensile strength (from 57 kPa up to 1.1 MPa) compared to the matrix polymers without affecting the elongation at break. Furthermore, an increased thermal stability from 40 °C to 85 °C of the networks could be demonstrated. The differences of the behaviour of the functionalized gelatins to pure gelatin as matrix suggested an additional stabilizing bond between the incorporated aromatic groups to the hydroxyapatite as supported by the IR results. The composites can potentially be applied as bone fillers.}, note = {Online available at: \url{https://doi.org/10.1016/j.actbio.2010.11.025} (DOI). Neffe, A.; Loebus, A.; Zaupa, A.; Stoetzel, C.; Mueller, F.; Lendlein, A.: Gelatin Functionalization with Tyrosine Derived Moieties for Increasing the Interaction with Hydroxyapatite Fillers. Acta Biomaterialia. 2011. vol. 7, no. 4, 1693-1701. DOI: 10.1016/j.actbio.2010.11.025}} @misc{zaupa_a_molecular_2011, author={Zaupa, A., Neffe, A.T., Pierce, B.F., Lendlein, A., Hofmann, D.}, title={A molecular dynamic analysis of gelatin as an amorphous material: Prediction of mechanical properties of gelatin systems}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.5301/IJAO.2010.6083}, abstract = {Biomaterials are used in regenerative medicine for induced autoregeneration and tissue engineering. This is often challenging, however, due to difficulties in tailoring and controlling the respective material properties. Since functionalization is expected to offer better control, in this study gelatin chains were modified with physically interacting groups based on tyrosine with the aim of causing the formation of physical crosslinks. This method permits application-specific properties like swelling and better tailoring of mechanical properties. The design of the crosslink strategy was supported by molecular dynamic (MD) simulations of amorphous bulk models for gelatin and functionalized gelatins at different water contents (0.8 and 25 wt.-%). The results permitted predictions to be formulated about the expected crosslink density and its influence on equilibrium swelling behavior and on elastic material properties. The models of pure gelatin were used to validate the strategy by comparison between simulated and experimental data such as density, backbone conformation angle distribution, and X-ray scattering spectra. A key result of the simulations was the prediction that increasing the number of aromatic functions attached to the gelatin chain leads to an increase in the number of physical netpoints observed in the simulated bulk packing models. By comparison with the Flory-Rehner model, this suggested reduced equilibrium swelling of the functionalized materials in water, a prediction that was subsequently confirmed by our experimental work. The reduction and control of the equilibrium degree of swelling in water is a key criterion for the applicability of functionalized gelatins when used, for example, as matrices for induced autoregeneration of tissues.}, note = {Online available at: \url{https://doi.org/10.5301/IJAO.2010.6083} (DOI). Zaupa, A.; Neffe, A.; Pierce, B.; Lendlein, A.; Hofmann, D.: A molecular dynamic analysis of gelatin as an amorphous material: Prediction of mechanical properties of gelatin systems. The International Journal of Artificial Organs. 2011. vol. 34, no. 2, 139-151. DOI: 10.5301/IJAO.2010.6083}} @misc{zaupa_influence_of_2011, author={Zaupa, A., Neffe, A.T., Pierce, B.F., Noechel, U., Lendlein, A.}, title={Influence of Tyrosine-Derived Moieties and Drying Conditions on the Formation of Helices in Gelatin}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1021/bm101029k}, abstract = {The single and triple helical organization of protein chains strongly influences the mechanical properties of gelatin-based materials. A chemical method for obtaining different degrees of helical organization in gelatin is covalent functionalization, while a physical method for achieving the same goal is the variation of the drying conditions of gelatin solutions. Here we explored how the introduction of desaminotyrosine (DAT) and desaminotyrosyl tyrosine (DATT) linked to lysine residues of gelatin influenced the kinetics and thermodynamic equilibrium of the helicalization process of single and triple helices following different drying conditions. Drying at a temperature above the helix-to-coil transition temperature of gelatin (T > Tc, called vshort) generally resulted in gelatins with relatively lower triple helical content (Xc,t = 1−2%) than lower temperature drying (T < Tc, called vlong) (Xc,t = 8−10%), where the DAT(T) functional groups generally disrupted helix formation. While different helical contents affected the thermal transition temperatures only slightly, the mechanical properties were strongly affected for swollen hydrogels (E = 4−13 kPa for samples treated by vlong and E = 120−700 kPa for samples treated by vshort). This study shows that side group functionalization and different drying conditions are viable options to control the helicalization and macroscopic properties of gelatin-based materials.}, note = {Online available at: \url{https://doi.org/10.1021/bm101029k} (DOI). Zaupa, A.; Neffe, A.; Pierce, B.; Noechel, U.; Lendlein, A.: Influence of Tyrosine-Derived Moieties and Drying Conditions on the Formation of Helices in Gelatin. Biomacromolecules. 2011. vol. 12, no. 1, 75-81. DOI: 10.1021/bm101029k}} @misc{neffe_knowledgebased_design_2011, author={Neffe, A.T., Zaupa, A., Pierce, B.F., Lendlein, A.}, title={Knowledge-Based Design and Tailoring of Gelatin-Based Hydrogels by Functionalization with Tyrosine-Derived Groups}, year={2011}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Neffe, A.; Zaupa, A.; Pierce, B.; Lendlein, A.: Knowledge-Based Design and Tailoring of Gelatin-Based Hydrogels by Functionalization with Tyrosine-Derived Groups. MRS Fall 2011 Meeting. Boston, MA (USA), 2011.}} @misc{piece_photocrosslinked_conetworks_2011, author={Piece, B.F., Neffe, A.T., Lendlein, A.}, title={Photocrosslinked Co-Networks from Glycidylmethacrylated Gelatin and Poly(ethylene glycol) (Di)Methacrylates}, year={2011}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Piece, B.; Neffe, A.; Lendlein, A.: Photocrosslinked Co-Networks from Glycidylmethacrylated Gelatin and Poly(ethylene glycol) (Di)Methacrylates. MRS Fall 2011 Meeting, Symposium V. Boston, MA (USA), 2011.}} @misc{lange_synthesis_and_2011, author={Lange, M., Luetzow, K., Neffe, A.T., Lendlein, A.}, title={Synthesis and Characterization of a new Poly(ether imide) Based on 3-Methoxy-1,2-propandiol}, year={2011}, howpublished = {conference poster: Twente (NL);}, note = {Lange, M.; Luetzow, K.; Neffe, A.; Lendlein, A.: Synthesis and Characterization of a new Poly(ether imide) Based on 3-Methoxy-1,2-propandiol. In: Advanced Functional Polymers for Medicine, AFPM 2011. Twente (NL). 2011.}} @misc{neffe_physical_crosslinking_2011, author={Neffe, A.T., Zaupa, A., Lendlein, A.}, title={Physical Crosslinking of Gelatin: A Supramolecular Approach to Biomaterials}, year={2011}, howpublished = {conference poster: Porto (P);}, note = {Neffe, A.; Zaupa, A.; Lendlein, A.: Physical Crosslinking of Gelatin: A Supramolecular Approach to Biomaterials. In: 38th Congress of the European Society for Artificial Organs, ESAO 2011. Porto (P). 2011.}} @misc{santan_thermal_gelation_2011, author={Santan, H.D., Neffe, A.T., Kamlage, S., Lendlein, A.}, title={Thermal Gelation and Stability of Pectin Grafted with PEPE}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2012.703}, abstract = {Stimuli-sensitive materials can change properties upon exposure to an external stimulus. Thermoreversible gelation upon heating is one example for such a stimuli sensitivity. Here, it is of significance to tailor the tAransition temperature and to achieve large changes of G’ and the viscosity. Grafting of the thermosensitive poly(ethylene glycol-b-propylene glycol-b-ethylene glycol)s (PEPEs) to pectin was performed in order to investigate if tailoring of the sol-gel-transition temperature can be achieved by adjusting the grafting ratio. PEPEs were aminated and grafted to the polysaccharide via EDC coupling as shown by FTIR. The sol-gel transition of the pectin, PEPE, and the grafted system (PGP) was investigated by rheology. The gelation temperature (Tgel) of the system could be adjusted by varying the grafting density of PEPE onto pectin as well as by the concentration of the thermosensitive polymer in aqueous solution. A concentration of 15 – 20 wt% of the grafted system in water led to gelation temperatures in the range of 25 – 33 °C and the critical micelle concentration (CMC) and critical micelle temperature (CMT) of the grafted systems were determined by UV spectroscopy. The viscosity and the G’ increased by four orders of magnitudes at Tgel, which is comparable to PEPEs alone, but could be reached at lower PEPE concentrations. In the future, a thorough mechanistic investigation of the gelation process would be of interest.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2012.703} (DOI). Santan, H.; Neffe, A.; Kamlage, S.; Lendlein, A.: Thermal Gelation and Stability of Pectin Grafted with PEPE. MRS Online Proceedings Library. 2011. vol. 1403, 226-233. DOI: 10.1557/opl.2012.703}} @misc{santan_degradable_thermosensitive_2011, author={Santan, H.D., Neffe, A.T., Kamlage, S., Lendlein, A.}, title={Degradable Thermosensitive hydrogels based on grafted pectin or chondritin sulfate}, year={2011}, howpublished = {conference poster: Boston, MA (USA);}, note = {Santan, H.; Neffe, A.; Kamlage, S.; Lendlein, A.: Degradable Thermosensitive hydrogels based on grafted pectin or chondritin sulfate. In: MRS Fall 2011 Meeting, Symposium V. Boston, MA (USA). 2011.}} @misc{neffe_in_vitro_2011, author={Neffe, A.T., Kobuch, K.A., Maier, M., Feucht, N., Lohmann, C.P., Wolfstein, A., Streufert, D., Kamlage, S., Lendlein, A.}, title={In vitro and in vivo Evaluation of a Multifunctional Hyaluronic acid based Hydrogel System for Local Application on the Retina}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201100049}, abstract = {Conventional treatment of retinal detachment with laser and/or triamcinolon acetonide (TAAC) does not prevent loss of vision in all patients. Therefore, the development of degradable hydrogel patches covering retinal breaks was envisioned as alternative. Stable hydrogels could be formed by crosslinking hyaluronic acid with 1,2,3,4-diepoxybutane. Triamcinolone was diffusible in the gels. The hydrogels were slowly degrading, and mass loss during hydrolytic degradation was observed starting after three weeks. The sterilized gels showed excellent intraocular biocompatibility in vivo in rabbit eyes when applied as a patch on the retina. The good retinal adherence of the patch and absence of cellular growth and proliferation in and around the gel indicated the suitability as a material for a retinal patch to prevent cell-migration and proliferation after a retinal break and for local drug application.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201100049} (DOI). Neffe, A.; Kobuch, K.; Maier, M.; Feucht, N.; Lohmann, C.; Wolfstein, A.; Streufert, D.; Kamlage, S.; Lendlein, A.: In vitro and in vivo Evaluation of a Multifunctional Hyaluronic acid based Hydrogel System for Local Application on the Retina. Macromolecular Symposia. 2011. vol. 309-310, no. 1, 229-235. DOI: 10.1002/masy.201100049}} @misc{roch_reducing_the_2011, author={Roch, T., Pierce, B.F., Zaupa, A., Jung, F., Neffe, A.T.Lendlein, A.}, title={Reducing the Endotoxin Burden of Desaminotyrosine- and Desaminotyrosyl tyrosine-Functionalized Gelatin}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201100048}, abstract = {Biomaterial-induced autoregeneration requires materials with distinct tailored mechanical and thermal properties, water uptake and swelling properties as well as degradation behavior. Furthermore, before any biomaterial can be applied in vivo, in vitro studies should be performed that confirm the suitability for such applications. One facet in this process is the evaluation of endotoxin loads and immunogenic response to the material to avoid an unspecific activation of the immune system, which otherwise might cause fever and could lead to life–threatening pathologies. In this study, gelatins functionalized with desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT) were investigated in terms of their endotoxin content and their potential to induce an inflammatory cytokine response in macrophages. Using the Limulus amebocyte lysate (LAL) test it could be shown that the endotoxin content was substantially reduced by using certified low endotoxin containing gelatin and performing the gelatin functionalization under cleanroom conditions. Furthermore, production of inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor–alpha (TNFα) of an immune relevant macrophage cell line was significantly reduced for these materials. The survival of the macrophage cell line in the presence of DAT(T)-functionalized gelatins was not influenced by both materials. Therefore, DAT- and DATT-functionalized gelatins were shown to have passed the tests concerning immunological responses important for their applicability in vivo.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201100048} (DOI). Roch, T.; Pierce, B.; Zaupa, A.; Jung, F.; Neffe, A.: Reducing the Endotoxin Burden of Desaminotyrosine- and Desaminotyrosyl tyrosine-Functionalized Gelatin. Macromolecular Symposia. 2011. vol. 309-310, no. 1, 182-189. DOI: 10.1002/masy.201100048}} @misc{wischke_a_blend_2011, author={Wischke, C., Loebler, M., Neffe, A.T., Hanh, B.D., Zierke, M., Sternberg, K., Schmitz, K.-P., Guthoff, R., Lendlein, A.}, title={A Blend of Poly(Epsilon-caprolactone) and Poly[(Epsilon-caprolactone)-co-glycolide] with Remarkable Mechanical Features and Wide Applicability as Biomaterial}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201100050}, abstract = {Hydrolytic degradation of poly(ε-caprolactone) [PCL] can be enhanced by introduction of 8 wt.% glycolide leading to poly[(ε-caprolactone)-co-glycolide] (PCG), which has a low elongation at break εB of 4%. PCG/PCL blends (50/50 w/w) combined the advantageous features of its individual components such as mechanical properties similar to pure PCL (εB, Blend: 900 ± 230%; εB, PCL: 730 ± 50 at 20 °C), water uptake rates during degradation similar to pure PCG, and linear mass loss during bulk degradation independent from sample dimensions. The outcome of cytotoxicity studies was depending on the cell type with promising results, e.g., for Tenon fibroblasts. Easy processing of the blend was demonstrated by melt compression, foaming with CO2, and hot melt extrusion, suggesting a wide applicability as biomaterial, e.g., as drug carrier.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201100050} (DOI). Wischke, C.; Loebler, M.; Neffe, A.; Hanh, B.; Zierke, M.; Sternberg, K.; Schmitz, K.; Guthoff, R.; Lendlein, A.: A Blend of Poly(Epsilon-caprolactone) and Poly[(Epsilon-caprolactone)-co-glycolide] with Remarkable Mechanical Features and Wide Applicability as Biomaterial. Macromolecular Symposia. 2011. vol. 309-310, no. 1, 59-67. DOI: 10.1002/masy.201100050}} @misc{lange_synthesis_and_2011, author={Lange, M., Luetzow, K., Neffe, A.T., Lendlein, A.}, title={Synthesis and Characterization of Polyetherimides with 3-Methoxy-1,2-propanediol Moieties}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201100052}, abstract = {Polyetherimides have been evaluated as non-toxic and steam-sterilizable and are therefore potentially suited for biomedical applications. To enable a broader range of potential applications, polyetherimides with lower Tg, higher elasticity at room temperature and better processability are required. Our concept was to explore, whether the incorporation of 3-methoxy-1,2-propanediol moieties in the main chain lead to a reduction of Tg and increase the elastic properties of the polymer compared to commercially available polyetherimides from 4,4′-(4,4′-isopropylidenediphenoxy)bis(phthalic anhydride) and 1,3-diaminobenzene. Two different monomers were synthesized and co-condensated with each other or using 4,4,4′-(4,4′-isopropylidenediphenoxy)bis(phthalic anhydride), respectively. The results proofed the successful synthesis and polymerization leading to polymers with molecular weights up to Mn = 6,400 g/mol. The polymers showed lowered Tg, resistance to heat up to 400 °C, tendencies to reduced contact angles and partially reduced E-moduli in comparison to the commercial polyetherimide ULTEM 1000.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201100052} (DOI). Lange, M.; Luetzow, K.; Neffe, A.; Lendlein, A.: Synthesis and Characterization of Polyetherimides with 3-Methoxy-1,2-propanediol Moieties. Macromolecular Symposia. 2011. vol. 309-310, no. 1, 40-48. DOI: 10.1002/masy.201100052}} @misc{lange_polyetherimides_with_2011, author={Lange, M., Luetzow, K., Neffe, A.T., Lendlein, A.}, title={Polyetherimides with 3-methoxy-1,2-propanediol moieties}, year={2011}, howpublished = {conference poster: Ghent (B);}, note = {Lange, M.; Luetzow, K.; Neffe, A.; Lendlein, A.: Polyetherimides with 3-methoxy-1,2-propanediol moieties. In: Young European Biomaterial Scientists Designing a View for the Future, BioFuture 2011. Ghent (B). 2011.}} @misc{wischke_a_blend_2011, author={Wischke, C., Loebler, M., Neffe, A.T., Hanh, B.D., Zierke, M., Sternberg, K., Schmitz, K.-P., Guthoff, R., Lendlein, A.}, title={A Blend of Poly(Epsilon-caprolactone) and Poly[(Epsilon-caprolactone)-co-glycolide] with Remarkable Mechanical Features and Wide Applicability as Biomaterial}, year={2011}, howpublished = {conference poster: Twente (NL);}, note = {Wischke, C.; Loebler, M.; Neffe, A.; Hanh, B.; Zierke, M.; Sternberg, K.; Schmitz, K.; Guthoff, R.; Lendlein, A.: A Blend of Poly(Epsilon-caprolactone) and Poly[(Epsilon-caprolactone)-co-glycolide] with Remarkable Mechanical Features and Wide Applicability as Biomaterial. In: Advanced Functional Polymers for Medicine, AFPM 2011. Twente (NL). 2011.}} @misc{piluso_synthesis_and_2011, author={Piluso, S., Lendlein, A., Neffe, A.T.}, title={Synthesis and Characterization of Gelatin Fragments Obtained by Controlled Degradation}, year={2011}, howpublished = {conference poster: Ghent (B);}, note = {Piluso, S.; Lendlein, A.; Neffe, A.: Synthesis and Characterization of Gelatin Fragments Obtained by Controlled Degradation. In: Young European Biomaterial Scientists Designing a View for the Future, BioFuture 2011. Ghent (B). 2011.}} @misc{piluso_synthesis_and_2011, author={Piluso, S., Weigel, T., Lendlein, A., Neffe, A.T.}, title={Synthesis and Characterization of Gelatin Fragments Obtained by Controlled Degradation}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201100054}, abstract = {Telechelic oligomers are attractive starting materials for the preparation of materials with tailorable properties. Here, peptide chains with defined molecular weight were obtained by controlled degradation of gelatin with hydroxylamine, which resulted in the cleavage of asparaginyl-glycine bonds and formation of new aspartyl hydroxamates and amino endgroups. The reaction of gelatin with hydroxylamine resulted in fragments with molecular weights of 15, 25, 37, and 50 kDa (determined by SDS-PAGE) independently of the reaction time and conditions. The fragment mixture showed typical single and triple helical organization in WAXS spectra, but rheological studies showed lower G′ and G″ values for gels from the fragment mixture than from gelatin, and lower gel-sol transitions temperatures. A more narrow distribution was found for the fragment mixture (Mn=25 kDa, PDI=1.4) than for commercial gelatin.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201100054} (DOI). Piluso, S.; Weigel, T.; Lendlein, A.; Neffe, A.: Synthesis and Characterization of Gelatin Fragments Obtained by Controlled Degradation. Macromolecular Symposia. 2011. vol. 309-310, no. 1, 199-204. DOI: 10.1002/masy.201100054}} @misc{piluso_synthesis_and_2011, author={Piluso, S., Lendlein, A., Neffe, A.T.}, title={Synthesis and Characterization of Gelatin Fragments Obtained by Controlled Degradation}, year={2011}, howpublished = {conference poster: Enschede (NL);}, note = {Piluso, S.; Lendlein, A.; Neffe, A.: Synthesis and Characterization of Gelatin Fragments Obtained by Controlled Degradation. In: Advanced Functional Polymers for Medicine, AFPM 2011. Enschede (NL). 2011.}} @misc{federico_synthesis_and_2011, author={Federico, S., Lendlein, A., Neffe, A.T.}, title={Synthesis and Characterization of a Telechelic Peptide as a Precursor for Supramolecular Networks}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1002/masy.201100055}, abstract = {A peptide showing propensity for the adoption of β-sheet conformation was synthesized in order to develop a defined molecular building block as part of a toolbox for the development of polymer networks based on supramolecular interactions. The peptide was synthesized by microwave-assisted solid phase peptide synthesis and the purification was performed by Reversed Phase-High Performance Liquid Chromatography (RP-HPLC), from which a purity higher than 95 mol-% was obtained. The calculated mass was confirmed by Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry (MALDI-ToF-MS). Further characterization of the peptide was performed by IR and 2D TOCSY, NOESY, and HSQC NMR spectroscopy, which confirmed the identity and the sequence of the peptide.}, note = {Online available at: \url{https://doi.org/10.1002/masy.201100055} (DOI). Federico, S.; Lendlein, A.; Neffe, A.: Synthesis and Characterization of a Telechelic Peptide as a Precursor for Supramolecular Networks. Macromolecular Symposia. 2011. vol. 309-310, no. 1, 205-212. DOI: 10.1002/masy.201100055}} @misc{federico_supramolecular_networks_2011, author={Federico, S., Lendlein, A., Neffe, A.T.}, title={Supramolecular Networks formed by Biological Rcognition of Collagen and a Peptide Grafted to Hyaluronic Acid}, year={2011}, howpublished = {conference poster: Enschede (NL);}, note = {Federico, S.; Lendlein, A.; Neffe, A.: Supramolecular Networks formed by Biological Rcognition of Collagen and a Peptide Grafted to Hyaluronic Acid. In: Advanced Functional Polymers for Medicine, AFPM 2011. Enschede (NL). 2011.}} @misc{neffe_quantifying_protein_2011, author={Neffe, A.T., Pierce, B.F., Blaszkiewicz, J., Lendlein, A.}, title={Quantifying Protein Adsorption to Physically Crosslinked Gelatin-Based Networks}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.1557/opl.2012.476}, abstract = {Physically crosslinked hydrogels based on gelatin functionalized with desaminotyrosine (DAT) (giving Gel-DAT) or desaminotyrosyl tyrosine (DATT) (resulting in Gel-DATT) have shown high potential as biomaterials. Here, protein adsorption to the functionalized gelatins in comparison to gelatin was quantified to see if the functionalization and chain organization of gelatins has an influence on the amount of proteins being adsorbed. For this purpose, gelatin, Gel-DAT, and Gel-DATT were incubated with water or aq. solutions of bovine serum albumin (BSA), fibrinogen, or fibronectin, respectively, at physiological concentrations. Protein concentrations in the supernatant were determined with the bicinchoninic acid (BCA) assay before and after the contact. BSA adsorption to the materials was influenced as well by the hydrophobicity of the material as the degree of swelling, with the observation that higher protein concentrations led to lower protein adsorption. The highest amount of fibronectin was adsorbed to Gel-DAT, followed by gelatin and Gel-DATT, with only small differences for different initial protein concentrations. Fibrinogen adsorption increased with increasing concentration. In the future, adsorption studies based on specific antibody-based techniques might enable quantification of the proteins also in competition assays and direct quantification of adsorbed material.}, note = {Online available at: \url{https://doi.org/10.1557/opl.2012.476} (DOI). Neffe, A.; Pierce, B.; Blaszkiewicz, J.; Lendlein, A.: Quantifying Protein Adsorption to Physically Crosslinked Gelatin-Based Networks. MRS Online Proceedings Library. 2011. vol. 1403, 196-201. DOI: 10.1557/opl.2012.476}} @misc{piluso_hyaluronic_acidbased_2011, author={Piluso, S., Hiebl, B., Gorb, S.N., Kovalev, A., Lendlein, A., Neffe, A.T.}, title={Hyaluronic acid-based hydrogels crosslinked by copper-catalyzed azide-alkyne cycloaddition with tailorable mechanical properties}, year={2011}, howpublished = {journal article}, doi = {https://doi.org/10.5301/IJAO.2011.6394}, abstract = {Biopolymers of the extracellular matrix are attractive starting materials for providing degradable and biocompatible biomaterials. In this study, hyaluronic acid-based hydrogels with tunable mechanical properties were prepared by the use of copper- catalyzed azide-alkyne cycloaddition (known as “click chemistry”). Alkyne-functionalized hyaluronic acid was crosslinked with linkers having two terminal azide functionalities, varying crosslinker density as well as the lengths and rigidity of the linker molecules. By variation of the crosslinker density and crosslinker type, hydrogels with elastic moduli in the range of 0.5-4 kPa were prepared. The washed materials contained a maximum of 6.8 mg copper per kg dry weight and the eluate of the gel crosslinked with diazidostilbene did not show toxic effects on L929 cells. The hyaluronic acid-based hydrogels have potential as biomaterials for cell culture or soft tissue regeneration applications.}, note = {Online available at: \url{https://doi.org/10.5301/IJAO.2011.6394} (DOI). Piluso, S.; Hiebl, B.; Gorb, S.; Kovalev, A.; Lendlein, A.; Neffe, A.: Hyaluronic acid-based hydrogels crosslinked by copper-catalyzed azide-alkyne cycloaddition with tailorable mechanical properties. The International Journal of Artificial Organs. 2011. vol. 34, no. 2, 192-197. DOI: 10.5301/IJAO.2011.6394}} @misc{wischke_multifunctional_polymers_2010, author={Wischke, C., Neffe, A.T., Lendlein, A.}, title={Multifunctional polymers combining shape-memory, drug release, and biodegradability}, year={2010}, howpublished = {conference lecture: Bad Honnef (D);}, note = {Wischke, C.; Neffe, A.; Lendlein, A.: Multifunctional polymers combining shape-memory, drug release, and biodegradability. Biodegradable Polymers as Biomaterials, 459th WE-Heraeus-Seminar. Bad Honnef (D), 2010.}} @misc{wischke_controlled_drug_2010, author={Wischke, C., Neffe, A., Lendlein, A.}, title={Controlled Drug Release from Biodegradable Shape-Memory Polymers}, year={2010}, howpublished = {book part}, doi = {https://doi.org/10.1007/12_2009_29}, abstract = {of drugs. This chapter provides a detailed description of the molecular basis for such multifunctional SMPs including the selection of building blocks, the polymer morphology, and the three dimensional architecture. Moreover, drug loading and release, drug effects on thermomechanical properties of SMPs, and drug release patterns in a physiological environment are described and potential applications in minimally-invasive surgery are discussed.}, note = {Online available at: \url{https://doi.org/10.1007/12_2009_29} (DOI). Wischke, C.; Neffe, A.; Lendlein, A.: Controlled Drug Release from Biodegradable Shape-Memory Polymers. In: Lendlein, A. (Ed.): Advances in Polymer Science - Shape-memory Polymers. Berlin/Heidelberg: Springer. 2010. 177-206. DOI: 10.1007/12_2009_29}} @misc{neffe_controlled_change_2010, author={Neffe, A.T., Tronci, G., Alteheld, A., Lendlein, A.}, title={Controlled Change of Mechanical Properties during Hydrolytic Degradation of Polyester Urethane Networks}, year={2010}, howpublished = {journal article}, doi = {https://doi.org/10.1002/macp.200900441}, abstract = {Polyester urethane networks are versatile polymer systems as it is possible to tailor their mechanical properties and their hydrolytic degradation profile. For biomedical applications, the biodegradability as well as the thermomechanical properties of the polymer networks during the course of degradation is of importance. Therefore, we investigated the change of thermomechanical properties of networks based on star-shaped precursors of rac-dilactide and diglycolide, -caprolactone, or p-dioxanone, respectively, during hydrolytic degradation. Degradation rate and mechanical properties of the polymer networks were tailored by crosslink density, comonomers, and by changing the glass transition temperature. Most importantly, the degradation of the networks led to a controlled, step-by-step change of the mechanical properties of the networks.}, note = {Online available at: \url{https://doi.org/10.1002/macp.200900441} (DOI). Neffe, A.; Tronci, G.; Alteheld, A.; Lendlein, A.: Controlled Change of Mechanical Properties during Hydrolytic Degradation of Polyester Urethane Networks. Macromolecular Chemistry and Physics. 2010. vol. 211, no. 2, 182-194. DOI: 10.1002/macp.200900441}} @misc{wischke_understanding_instability_2010, author={Wischke, C., Weigel, J., Neffe, A.T., Lendlein, A.}, title={Understanding instability and rupture of polyalkyl 2-cyanoacrylate capsules}, year={2010}, howpublished = {conference poster: Bad Honnef (D);}, note = {Wischke, C.; Weigel, J.; Neffe, A.; Lendlein, A.: Understanding instability and rupture of polyalkyl 2-cyanoacrylate capsules. In: Biodegradable Polymers as Biomaterials, 459th WE-Heraeus-Seminar. Bad Honnef (D). 2010.}} @misc{wischke_copolyesterdimethacrylatederived_networks_2010, author={Wischke, C., Neffe, A.T., Lendlein, A.}, title={Copolyesterdimethacrylate-derived Networks combine Shape-memory, Biodegradability, and Controlled Drug Release}, year={2010}, howpublished = {conference poster: Berlin (D);}, note = {Wischke, C.; Neffe, A.; Lendlein, A.: Copolyesterdimethacrylate-derived Networks combine Shape-memory, Biodegradability, and Controlled Drug Release. In: GDCh Biannual Meeting of the GDCh-Division Macromolecular Chemistry and Polydays. Berlin (D). 2010.}} @misc{wischke_comparing_techniques_2010, author={Wischke, C., Neffe, A.T., Steuer, S., Lendlein, A.}, title={Comparing techniques for drug loading of shape-memory polymer networks–effect on their functionalities}, year={2010}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.ejps.2010.06.003}, abstract = {A family of oligo[(var epsilon-caprolactone)-co-glycolide]dimethacrylate (oCG-DMA) derived networks of different glycolide content as well as precursor molecular weight has been synthesized by crosslinking oCG-DMA, providing matrices of different hydrophilicity, network density, and morphology at body temperature. Such networks were loaded with a hydrophilic model drug, ethacridine lactate, either before crosslinking or afterwards by swelling in drug solution. Disadvantageous alterations of the shape-memory functionality and degradation characteristics were observed only in few loaded materials. Loading by swelling generally resulted in low payloads, which slightly increased for more hydrophilic polymer networks, and a substantial burst and fast subsequent release for all investigated materials. Loading before crosslinking gave almost no burst and higher subsequent release rates over longer periods of time. Overall, depending on the needs of a specific application, a material from this polymer family with the desired mechanical properties, shape-memory functionality, and degradation pattern can be selected and combined with drugs when considering that i) loading by swelling is best suited for applications that require high initial doses and ii) loading before crosslinking allows easy variation of payloads and low burst release for therapeutics that are non-sensitive to chemical alterations during crosslinking.}, note = {Online available at: \url{https://doi.org/10.1016/j.ejps.2010.06.003} (DOI). Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Comparing techniques for drug loading of shape-memory polymer networks–effect on their functionalities. European Journal of Pharmaceutical Sciences. 2010. vol. 41, no. 11, 136-147. DOI: 10.1016/j.ejps.2010.06.003}} @misc{neffe_abbaubare_polymere_2010, author={Neffe, A.T., Lendlein, A.}, title={Abbaubare Polymere als Biomaterialien}, year={2010}, howpublished = {journal article}, abstract = {No abstract}, note = {Neffe, A.; Lendlein, A.: Abbaubare Polymere als Biomaterialien. Physik-Journal. 2010. vol. 9, no. 10, 56.}} @misc{luetzow_developing_cell_2010, author={Luetzow, K., Neffe, A., Lendlein, A.}, title={Developing Cell Selectivities of Acrylonitrile Based Copolymers and Porous Bodies from Poly(ether imide)}, year={2010}, howpublished = {conference paper: Minneapolis, MN (USA);}, note = {Luetzow, K.; Neffe, A.; Lendlein, A.: Developing Cell Selectivities of Acrylonitrile Based Copolymers and Porous Bodies from Poly(ether imide). In: Medical Device Materials V, Proceedings from the Materials Processes for Medical Devices Conference, MPMD 2009. Minneapolis, MN (USA). ASM International. 2010. 169-174.}} @misc{tronci_an_entropyelastic_2010, author={Tronci, G., Neffe, A.T., Pierce, B.F., Lendlein, A.}, title={An Entropy–Elastic Gelatin-based Hydrogel System}, year={2010}, howpublished = {journal article}, doi = {https://doi.org/10.1039/C0JM00883D}, abstract = {Gelatin is a non-immunogenic and degradable biopolymer, which is widely applied in the biomedical field e.g. for drug capsules or as absorbable hemostats. However, gelatin materials present limited and hardly reproducible mechanical properties especially in aqueous systems, particularly caused by the uncontrollable partial renaturation of collagen-like triple helices. Therefore, mechanically demanding applications for gelatin-based materials, such as vascular patches, i.e. hydrogel films that seal large incisions in vessel walls, and for induced autoregeneration, are basically excluded if this challenge is not addressed. Through the synthesis of a defined chemical network of gelatin with hexamethylene diisocyanate (HDI) in DMSO, the self-organization of gelatin chains could be hindered and amorphous gelatin films were successfully prepared having Young's moduli of 60–530 kPa. Transferring the crosslinking reaction with HDI and, alternatively, ethyl lysine diisocyanate (LDI), to water as reaction medium allowed the tailoring of swelling behaviour and mechanical properties by variation of crosslinker content while suppressing the formation of helices. The hydrogels had Young's moduli of 70–740 kPa, compressive moduli of 16–48 kPa, and degrees of swelling of 300–800 vol%. Test reactions investigated by ESI mass spectrometry allowed the identification and quantification of reaction products of the crosslinking reaction. The HDI crosslinked networks were stabilized by direct covalent crosslinks (ca. 10 mol%), supported by grafting (50 mol%) and blending of hydrophobic oligomeric chains. For the LDI-based networks, less crosslinked (3 mol%) and grafted species (5 mol%) and much higher amounts of oligomers were observed. The adjustable hydrogel system enables the application of gelatin-based materials in physiological environments.}, note = {Online available at: \url{https://doi.org/10.1039/C0JM00883D} (DOI). Tronci, G.; Neffe, A.; Pierce, B.; Lendlein, A.: An Entropy–Elastic Gelatin-based Hydrogel System. Journal of Materials Chemistry. 2010. vol. 20, no. 40, 8875-8884. DOI: 10.1039/C0JM00883D}} @misc{neffe_knowledgebased_tailoring_2010, author={Neffe, A.T., Zaupa, A., Pierce, B.F., Hofmann, D., Lendlein, A.}, title={Knowledge-Based Tailoring of Gelatin-Based Materials by Functionalization with Tyrosine-Derived Groups}, year={2010}, howpublished = {journal article}, doi = {https://doi.org/10.1002/marc.201000274}, abstract = {Molecular models of gelatin-based materials formed the basis for the knowledge-based design of a physically cross-linked polymer system. The computational models with 25 wt.-% water content were validated by comparison of the calculated structural properties with experimental data and were then used as predictive tools to study chain organization, cross-link formation, and estimation of mechanical properties. The introduced tyrosine-derived side groups, desaminotyrosine (DAT) and desaminotyrosyl tyrosine (DATT), led to the reduction of the residual helical conformation and to the formation of physical net-points by π–π interactions and hydrogen bonds. At 25 wt.-% water content, the simulated and experimentally determined mechanical properties were in the same order of magnitude. The degree of swelling in water decreased with increasing the number of inserted aromatic functions, while Young's modulus, elongation at break, and maximum tensile strength increased.}, note = {Online available at: \url{https://doi.org/10.1002/marc.201000274} (DOI). Neffe, A.; Zaupa, A.; Pierce, B.; Hofmann, D.; Lendlein, A.: Knowledge-Based Tailoring of Gelatin-Based Materials by Functionalization with Tyrosine-Derived Groups. Macromolecular Rapid Communications. 2010. vol. 31, no. 17, 1534-1539. DOI: 10.1002/marc.201000274}} @misc{wischke_abpolymer_networks_2010, author={Wischke, C., Neffe, A.T., Steuer, S., Engelhardt, E., Lendlein, A.}, title={AB-polymer Networks with Cooligoester and Poly(Eta-butyl acrylate) Segments as a Multifunctional Matrix for Controlled Drug Release}, year={2010}, howpublished = {journal article}, doi = {https://doi.org/10.1002/mabi.201000089}, abstract = {Semi-crystalline AB-copolymer networks from oligo[(ε-caprolactone)-co-glycolide]dimethacrylates and n-butylacrylate have recently been shown to exhibit a shape-memory functionality, which may be used for self-deploying and anchoring of implants. In this study, a family of such materials differing in their molar glycolide contents χG was investigated to determine structure–property functional relationships of unloaded and drug loaded specimens. Drug loading and release were evaluated, as well as their degradation behavior in vitro and in vivo. Higher χG resulted in higher loading levels by swelling and a faster release of ethacridine lactate, lower melting temperature of polymer crystallites, and a decrease in shape fixity ratio of the programmed temporary shape. For unloaded networks, the material behavior in vivo was independent of the mechanical load associated with different implantation sites and agreed well with data from in vitro degradation studies. Thus, AB networks could be used as novel matrices for biofunctional implants, e.g., for urogenital applications, which can self-anchor in vivo and provide mechanical support, release drugs, and finally degrade in the body to excretable fragments.}, note = {Online available at: \url{https://doi.org/10.1002/mabi.201000089} (DOI). Wischke, C.; Neffe, A.; Steuer, S.; Engelhardt, E.; Lendlein, A.: AB-polymer Networks with Cooligoester and Poly(Eta-butyl acrylate) Segments as a Multifunctional Matrix for Controlled Drug Release. Macromolecular Bioscience. 2010. vol. 10, no. 9, 1063-1072. DOI: 10.1002/mabi.201000089}} @misc{zaupa_knowledgebased_approach_2010, author={Zaupa, A., Neffe, A.T., Pierce, B.F., Hofmann, D., Lendlein, A.}, title={Knowledge-Based Approach to Tailorable Gelatin-Based Materials Functionalized with Tyrosine-Derived Moieties}, year={2010}, howpublished = {conference poster: Berlin (D);}, note = {Zaupa, A.; Neffe, A.; Pierce, B.; Hofmann, D.; Lendlein, A.: Knowledge-Based Approach to Tailorable Gelatin-Based Materials Functionalized with Tyrosine-Derived Moieties. In: Polymers in Biomedicine and Electronics. Berlin (D). 2010.}} @misc{piluso_hyaluronic_acidbased_2010, author={Piluso, S., Lendlein, A., Neffe, A.T.}, title={Hyaluronic acid-based hydrogels crosslinked by copper-catalyzed azide-alkyne cycloaddition with tailorable mechanical properties}, year={2010}, howpublished = {conference poster: Bad Honnef (D);}, note = {Piluso, S.; Lendlein, A.; Neffe, A.: Hyaluronic acid-based hydrogels crosslinked by copper-catalyzed azide-alkyne cycloaddition with tailorable mechanical properties. In: Biodegradable Polymers as Biomaterials, 459th WE-Heraeus-Seminar. Bad Honnef (D). 2010.}} @misc{wischke_amorphous_polymer_2009, author={Wischke, C., Neffe, A., Steuer, S., Lendlein, A.}, title={Amorphous Polymer Networks Combining Three Functionalities - Shape-Memory, Biodegradability, and Drug Release}, year={2009}, howpublished = {conference paper: San Francisco, CA (USA);}, doi = {https://doi.org/10.1557/PROC-1190-NN11-34}, note = {Online available at: \url{https://doi.org/10.1557/PROC-1190-NN11-34} (DOI). Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Amorphous Polymer Networks Combining Three Functionalities - Shape-Memory, Biodegradability, and Drug Release. In: Lendlein, A.; Prasad Shastri, V.; Gall, K. (Ed.): Active Polymers - MRS Symposium Proceedings, MRS Spring Meeting 2009. San Francisco, CA (USA). 2009. NN11-34. DOI: 10.1557/PROC-1190-NN11-34}} @misc{abell_molecular_modeling_2009, author={Abell, A.D., Jones, M.A., Coxon, J.M., Morton, J.D., Aitken, S.G., Mc Nabb, S.B., Lee, H.Y.Y., Mehrtens, J.M., Alexander, N.A., Stuart, B.G., Neffe, A.T., Bickerstaffe, R.}, title={Molecular Modeling, Synthesis, and Biological Evaluation of Macrocyclic Calpain Inhibitors}, year={2009}, howpublished = {journal article}, doi = {https://doi.org/10.1002/ange.200805014}, abstract = {Entwurf und Synthese einer Reihe von makrocyclischen Gerüsten, die eine -Strang-ähnliche Peptidrückgrat-Konformation bevorzugen, führten zu wirksamen und selektiven Inhibitoren von Calpain 2. Der Makrocyclus 1 verzögerte die calciuminduzierte Eintrübung von Schafslinsen in Kultur und dient als Leitstruktur für die Entwicklung eines Wirkstoffs zur Behandlung von grauem Star.}, note = {Online available at: \url{https://doi.org/10.1002/ange.200805014} (DOI). Abell, A.; Jones, M.; Coxon, J.; Morton, J.; Aitken, S.; Mc Nabb, S.; Lee, H.; Mehrtens, J.; Alexander, N.; Stuart, B.; Neffe, A.; Bickerstaffe, R.: Molecular Modeling, Synthesis, and Biological Evaluation of Macrocyclic Calpain Inhibitors. Angewandte Chemie. 2009. vol. 121, no. 8, 1483-1486. DOI: 10.1002/ange.200805014}} @misc{wischke_amorphous_polymer_2009, author={Wischke, C., Neffe, A., Steuer, S., Lendlein, A.}, title={Amorphous Polymer Network combining Three Functionalities – Shape-memory, Biodegradability, and Drug Release}, year={2009}, howpublished = {conference lecture: Kopenhagen (DK);}, note = {Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Amorphous Polymer Network combining Three Functionalities – Shape-memory, Biodegradability, and Drug Release. 36th Annual Meeting and Exposition of the Controlled Release Society. Kopenhagen (DK), 2009.}} @misc{neffe_polymer_networks_2009, author={Neffe, A.T., Steuer, S., Hanh, B.-D., Lendlein, A.}, title={Polymer networks combining three functionalities – shape-memory, biodegradability, and drug release}, year={2009}, howpublished = {conference lecture: Bangkok (THA);}, note = {Neffe, A.; Steuer, S.; Hanh, B.; Lendlein, A.: Polymer networks combining three functionalities – shape-memory, biodegradability, and drug release. 4th World Congress on Regenerative Medicine. Bangkok (THA), 2009.}} @misc{neffe_polymer_networks_2009, author={Neffe, A.T., Hanh, B.D., Steuer, S., Lendlein, A.}, title={Polymer Networks Combining Controlled Drug Release, Biodegradation, and Shape Memory Capability}, year={2009}, howpublished = {journal article}, doi = {https://doi.org/10.1002/adma.200802333}, abstract = {No abstract}, note = {Online available at: \url{https://doi.org/10.1002/adma.200802333} (DOI). Neffe, A.; Hanh, B.; Steuer, S.; Lendlein, A.: Polymer Networks Combining Controlled Drug Release, Biodegradation, and Shape Memory Capability. Advanced Materials. 2009. vol. 21, no. 32-33, 3394-3398. DOI: 10.1002/adma.200802333}} @misc{luetzow_developing_cell_2009, author={Luetzow, K., Neffe, A., Lendlein, A.}, title={Developing Cell Selectivities of Acrylonitrile Based Copolymers and Porous Bodies from Poly(ether imide)}, year={2009}, howpublished = {conference lecture: Minneapolis, MN (USA);}, note = {Luetzow, K.; Neffe, A.; Lendlein, A.: Developing Cell Selectivities of Acrylonitrile Based Copolymers and Porous Bodies from Poly(ether imide). Materials Processes for Medical Devices Conference, MPMD 2009. Minneapolis, MN (USA), 2009.}} @misc{wischke_evaluation_of_2009, author={Wischke, C., Neffe, A., Steuer, S., Lendlein, A.}, title={Evaluation of a degradable shape-memory polymer network as matrix for controlleddrug release}, year={2009}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.jconrel.2009.05.027}, abstract = {functionalities, i.e., a shape-memory effect combined with biodegradability and controlled drug release.}, note = {Online available at: \url{https://doi.org/10.1016/j.jconrel.2009.05.027} (DOI). Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Evaluation of a degradable shape-memory polymer network as matrix for controlleddrug release. Journal of Controlled Release. 2009. vol. 138, no. 3, 243-250. DOI: 10.1016/j.jconrel.2009.05.027}} @misc{wischke_amorphous_polymer_2009, author={Wischke, C., Neffe, A., Steuer, S., Lendlein, A.}, title={Amorphous Polymer Networks Combining Three Functionalities - Shape-Memory, Biodegradability, and Drug Release}, year={2009}, howpublished = {conference lecture: San Francisco, CA (USA);}, note = {Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Amorphous Polymer Networks Combining Three Functionalities - Shape-Memory, Biodegradability, and Drug Release. MRS Spring Meeting 2009. San Francisco, CA (USA), 2009.}} @misc{neffe_introduction_to_2009, author={Neffe, A.T., Lendlein, A.}, title={Introduction to Peptide Synthesis}, year={2009}, howpublished = {lecture: Universitaet Potsdam, FB Chemie;}, note = {Neffe, A.; Lendlein, A.: Introduction to Peptide Synthesis. Universitaet Potsdam, FB Chemie, 2009.}} @misc{wischke_amorphous_polymer_2009, author={Wischke, C., Neffe, A., Steuer, S., Lendlein, A.}, title={Amorphous polymer networks combining three functionalities – Shape-memory, biodegradability, and drug release}, year={2009}, howpublished = {conference poster: Kopenhagen (DK);}, note = {Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Amorphous polymer networks combining three functionalities – Shape-memory, biodegradability, and drug release. In: Controlled Release Society Annual Meeting & Exposition 2009. Kopenhagen (DK). 2009.}} @misc{lendlein_biomaterials_and_2009, author={Lendlein, A., Luetzow, K., Hiebl, B., Lange, M., Weigel, T., Seifert, B., Klein, F., Tronci, G., Neffe, A.}, title={Biomaterials and Scaffolds for Tissue Regeneration}, year={2009}, howpublished = {conference lecture (invited): Potsdam (D);}, note = {Lendlein, A.; Luetzow, K.; Hiebl, B.; Lange, M.; Weigel, T.; Seifert, B.; Klein, F.; Tronci, G.; Neffe, A.: Biomaterials and Scaffolds for Tissue Regeneration. Organotyp Tissue Culture Techniques for Substance Evaluation, DECHEMA/BioTop Potsdam Symposium. Potsdam (D), 2009.}} @misc{neffe_bioabbaubare_polymernetzwerke_2009, author={Neffe, A.T.}, title={Bioabbaubare Polymernetzwerke mit kontrollierter Wirkstofffreisetzung und Formgedaechtnis}, year={2009}, howpublished = {conference lecture: Hannover (D);}, note = {Neffe, A.: Bioabbaubare Polymernetzwerke mit kontrollierter Wirkstofffreisetzung und Formgedaechtnis. 18. Nachwuchswissenschaftler-Symposium Bioorganische Chemie. Hannover (D), 2009.}} @misc{neffe_wirkstoffdesign_und_2009, author={Neffe, A.T., Lendlein, A.}, title={Wirkstoffdesign und Freisetzungssysteme}, year={2009}, howpublished = {lecture: Universitaet Potsdam, FB Chemie;}, note = {Neffe, A.; Lendlein, A.: Wirkstoffdesign und Freisetzungssysteme. Universitaet Potsdam, FB Chemie, 2009.}} @misc{neffe_neuer_therapieansatz_2009, author={Neffe, A.T.}, title={Neuer Therapieansatz in der Glaukombehandlung - Drainagesystem als Grundlage fuer das trabekelwerk-Tissue-Engineering}, year={2009}, howpublished = {conference lecture (invited): Hannover (D);}, note = {Neffe, A.: Neuer Therapieansatz in der Glaukombehandlung - Drainagesystem als Grundlage fuer das trabekelwerk-Tissue-Engineering. 3. BMBF-Projektforum Biotechnologie. Hannover (D), 2009.}} @misc{neffe_thermomechanical_properties_2009, author={Neffe, A.T., Hanh, B.D., Steuer, S., Wischke, C., Lendlein, A.}, title={Thermomechanical Properties and Shape-Memory Capability of Drug Loaded Semi-Crystalline Polyestermethacrylate Networks}, year={2009}, howpublished = {conference poster: San Francisco, CA (USA);}, note = {Neffe, A.; Hanh, B.; Steuer, S.; Wischke, C.; Lendlein, A.: Thermomechanical Properties and Shape-Memory Capability of Drug Loaded Semi-Crystalline Polyestermethacrylate Networks. In: MRS Spring Meeting 2009. San Francisco, CA (USA). 2009.}} @misc{neffe_thermomechanical_properties_2009, author={Neffe, A.T., Hanh, B.D., Steuer, S., Wischke, C., Lendlein, A.}, title={Thermomechanical Properties and Shape-Memory Capability of Drug Loaded Semi-Crystalline Polyestermethacrylate Networks}, year={2009}, howpublished = {conference paper: San Francisco, CA (USA);}, doi = {https://doi.org/10.1557/PROC-1190-NN06-02}, note = {Online available at: \url{https://doi.org/10.1557/PROC-1190-NN06-02} (DOI). Neffe, A.; Hanh, B.; Steuer, S.; Wischke, C.; Lendlein, A.: Thermomechanical Properties and Shape-Memory Capability of Drug Loaded Semi-Crystalline Polyestermethacrylate Networks. In: Lendlein, A.; Prasad Shastri, V.; Gall, K. (Ed.): Active Polymers - MRS Symposium Proceedings, MRS Spring Meeting 2009. San Francisco, CA (USA). 2009. NN06-02. DOI: 10.1557/PROC-1190-NN06-02}} @misc{neffe_shapememory_polymers_2009, author={Neffe, A., Lendlein, A.}, title={Shape-Memory Polymers}, year={2009}, howpublished = {lecture: Freie Universität Berlin, FB Chemie;}, note = {Neffe, A.; Lendlein, A.: Shape-Memory Polymers. Freie Universität Berlin, FB Chemie, 2009.}} @misc{lendlein_biomimetic_scaffolds_2009, author={Lendlein, A., Tronci, G., Zaupa, A., Pierce, B., Neffe, A., Cui, J., Kratz, K.}, title={Biomimetic Scaffolds supporting Bone Regeneration in Critical Defects}, year={2009}, howpublished = {conference lecture (invited): Berlin (D);}, note = {Lendlein, A.; Tronci, G.; Zaupa, A.; Pierce, B.; Neffe, A.; Cui, J.; Kratz, K.: Biomimetic Scaffolds supporting Bone Regeneration in Critical Defects. Biomechanics and Biology of Bone Healing, International Symposium 2009. Berlin (D), 2009.}} @misc{neffe_glycosylation_enhances_2008, author={Neffe, A.T., Meyer, B.}, title={Glycosylation Enhances the Calculated Binding Affinity of GP120 Derived Peptides and Peptidomimetics to CD4}, year={2008}, howpublished = {conference poster: Killarney (IRL);}, note = {Neffe, A.; Meyer, B.: Glycosylation Enhances the Calculated Binding Affinity of GP120 Derived Peptides and Peptidomimetics to CD4. In: Carbohydrates at the Interfaces of Biology, Medicine and Materials Science, 3rd EraChemistry Flash Conference. Killarney (IRL). 2008.}} @misc{jones_synthesis_biological_2008, author={Jones, M.A., Abell, A.D., Morton, J.D., Coxon, J.M., McNabb, S.B., Lee, H.Y.-Y., Aitken, S.G., Mehrtens, J.M., Robertson, L.J.G., Neffe, A.T., Gately, K., Wood, J.M.}, title={Synthesis, biological evaluation and molecular modeling of N-heterocyclic dipeptide aldehydes as selective calpain inhibitors}, year={2008}, howpublished = {journal article}, doi = {https://doi.org/10.1016/j.bmc.2008.05.048}, abstract = {A series of N-heterocyclic dipeptide aldehydes 4–13 have been synthesised and evaluated as inhibitors of ovine calpain 1 (o-CAPN1) and ovine calpain 2 (o-CAPN2). 5-Formyl-pyrrole 9 (IC50 values of 290 and 25 nM against o-CAPN1 and o-CAPN2, respectively) was the most potent and selective o-CAPN2 inhibitor, displaying >11-fold selectivity. The amino acid sequences of o-CAPN1 and o-CAPN2 have been determined. Because of the lack of available structural information on the ovine calpains, in silico homology models of the active site cleft of o-CAPN1 and o-CAPN2 were developed based on human calpain 1 (h-CAPN1) X-ray crystal structure (PDB code 1ZCM). These models were used to rationalise the observed SAR for compounds 4–13 and the selectivity observed for 9. The o-CAPN2 selective inhibitor 9 (CAT0059) was assayed in an in vitro ovine lens culture system and shown to successfully protect the lens from calcium-induced opacification.}, note = {Online available at: \url{https://doi.org/10.1016/j.bmc.2008.05.048} (DOI). Jones, M.; Abell, A.; Morton, J.; Coxon, J.; McNabb, S.; Lee, H.; Aitken, S.; Mehrtens, J.; Robertson, L.; Neffe, A.; Gately, K.; Wood, J.: Synthesis, biological evaluation and molecular modeling of N-heterocyclic dipeptide aldehydes as selective calpain inhibitors. Bioorganic & Medicinal Chemistry. 2008. vol. 16, no. 14, 6911-6923. DOI: 10.1016/j.bmc.2008.05.048}} @misc{kim_bayesian_multivariate_2008, author={Kim, S.W., Hudson, I.L., Neffe, A.T., Abell, A.D.}, title={Bayesian multivariate mixture (BMM) model: An upgraded classification method}, year={2008}, howpublished = {conference poster: Hamilton Island (AUS);}, note = {Kim, S.; Hudson, I.; Neffe, A.; Abell, A.: Bayesian multivariate mixture (BMM) model: An upgraded classification method. In: International Society for Bayesian Analysis, 9th World Meeting. Hamilton Island (AUS). 2008.}} @misc{lendlein_formation_of_2008, author={Lendlein, A., Luetzow, K., Madbouly, S., Weigel, T., Reiche, J., Kratz, K., Tronci, G., Neffe, A.T.}, title={Formation of Foams from Biodegradable Artificial Materials and Biopolymers}, year={2008}, howpublished = {conference lecture (invited): Berlin (D);}, note = {Lendlein, A.; Luetzow, K.; Madbouly, S.; Weigel, T.; Reiche, J.; Kratz, K.; Tronci, G.; Neffe, A.: Formation of Foams from Biodegradable Artificial Materials and Biopolymers. Biomechanics and Biology of Bone Healing, Symposium und Gruendung des Julius Wolff Instituts. Berlin (D), 2008.}} @misc{neffe_biopolymere_im_2008, author={Neffe, A.T.}, title={Biopolymere im Bereich biomedizinische Therapieentwicklungen}, year={2008}, howpublished = {conference lecture (invited): Luckenwalde (D);}, note = {Neffe, A.: Biopolymere im Bereich biomedizinische Therapieentwicklungen. Nachwachsende Rohstoffe in der Industrie, Kreativworkshop Biopolymere. Luckenwalde (D), 2008.}} @misc{neffe_dimensionally_stable_2008, author={Neffe, A.T., Tronci, G., Roessle, M., Lendlein, A.}, title={Dimensionally Stable Scaffolds from Gelatin-Based Polymer System}, year={2008}, howpublished = {conference lecture: Konstanz (D);}, note = {Neffe, A.; Tronci, G.; Roessle, M.; Lendlein, A.: Dimensionally Stable Scaffolds from Gelatin-Based Polymer System. 17. Nachwuchswissenschaftler-Symposium Bioorganische Chemie. Konstanz (D), 2008.}} @misc{neffe_wirkstoffdesign_und_2008, author={Neffe, A.T., Lendlein, A.}, title={Wirkstoffdesign und Freisetzungssysteme}, year={2008}, howpublished = {lecture: Universitaet Potsdam, FB Chemie;}, note = {Neffe, A.; Lendlein, A.: Wirkstoffdesign und Freisetzungssysteme. Universitaet Potsdam, FB Chemie, 2008.}} @misc{neffe_advanced_topics_2008, author={Neffe, A.T.}, title={Advanced Topics in Polymer Synthesis – Biopolymers}, year={2008}, howpublished = {lecture: FU Berlin, FB Chemie;}, note = {Neffe, A.: Advanced Topics in Polymer Synthesis – Biopolymers. FU Berlin, FB Chemie, 2008.}} @misc{neffe_tailored_scaffolds_2008, author={Neffe, A.T., Tronci, G., Roessle, M., Lendlein, A.}, title={Tailored Scaffolds from a Gelatin-based Polymer System: Influence of the Molecular Architecture on Material Properties}, year={2008}, howpublished = {conference poster: Boston, MA (USA);}, note = {Neffe, A.; Tronci, G.; Roessle, M.; Lendlein, A.: Tailored Scaffolds from a Gelatin-based Polymer System: Influence of the Molecular Architecture on Material Properties. In: MRS Fall Meeting 2008, SESSION HH5: Poster Session: Functional Materials and Drug Delivery Systems. Boston, MA (USA). 2008.}} @misc{neffe_triplefunctional_polymer_2008, author={Neffe, A.T., Hanh, B.D., Steuer, S., Lendlein, A.}, title={Triple-Functional Polymer Networks Combining Controlled Drug Release, Biodegradation, and Shape Memory Capability}, year={2008}, howpublished = {conference lecture: Boston, MA (USA);}, note = {Neffe, A.; Hanh, B.; Steuer, S.; Lendlein, A.: Triple-Functional Polymer Networks Combining Controlled Drug Release, Biodegradation, and Shape Memory Capability. MRS Fall Meeting 2008. Boston, MA (USA), 2008.}} @misc{neffe_triplefunctional_polymer_2008, author={Neffe, A.T., Hanh, B.D., Steuer, S., Kelch, S., Lendlein, A.}, title={Triple-Functional Polymer Networks Combining Controlled Drug Release, Biodegradation, and Shape Memory Capability}, year={2008}, howpublished = {conference lecture: Nuernberg (D);}, note = {Neffe, A.; Hanh, B.; Steuer, S.; Kelch, S.; Lendlein, A.: Triple-Functional Polymer Networks Combining Controlled Drug Release, Biodegradation, and Shape Memory Capability. Materials Science and Engineering, MSE 2008. Nuernberg (D), 2008.}} @misc{wischke_evaluation_of_2007, author={Wischke, C., Neffe, A., Steuer, S., Lendlein, A.}, title={Evaluation of a degradable shape-memory polymer network as matrix for controlleddrug release}, year={2007}, howpublished = {conference lecture: Toronto (CDN);}, abstract = {functionalities, i.e., a shape-memory effect combined with biodegradability and controlled drug release.}, note = {Wischke, C.; Neffe, A.; Steuer, S.; Lendlein, A.: Evaluation of a degradable shape-memory polymer network as matrix for controlleddrug release. 6th International Nanomedicine and Drug Delivery Symposium, Nano DDS 2008. Toronto (CDN), 2007.}} @misc{neffe_biopolymers__2007, author={Neffe, A.T.}, title={Biopolymers}, year={2007}, howpublished = {lecture: FU Berlin, FB Chemie;}, note = {Neffe, A.: Biopolymers. FU Berlin, FB Chemie, 2007.}} @misc{neffe_tailoring_established_2007, author={Neffe, A.T., Lendlein, A.}, title={Tailoring established polymers for medical applications}, year={2007}, howpublished = {journal article}, abstract = {Polymers can be tailored for specific biomedical applications by synthesis, processing or surface modification. Knowledge-based choice of comonomers for acrylonitrile based copolymers influences the interaction profile with specific cell lines and blood. Processing or surface modification of poly(ether imides) results in materials having complex three-dimensional structures and/or specific adsorption profiles. Potential applications are dialysis, gas separation, cell/tissue systems, apheresis, and bioreactors.}, note = {Neffe, A.; Lendlein, A.: Tailoring established polymers for medical applications. Medical Device Technology. 2007. vol. 18, no. 6, 14-19.}} @misc{neffe_design_synthesis_2007, author={Neffe, A.T., Meyer, B.}, title={Design, Synthesis, and Analysis of CD4 Binding Peptidomimetics – Development of HIV Entry Inhibitors}, year={2007}, howpublished = {conference lecture: Dortmund (D);}, note = {Neffe, A.; Meyer, B.: Design, Synthesis, and Analysis of CD4 Binding Peptidomimetics – Development of HIV Entry Inhibitors. 16. Nachwuchswissenschaftler-Symposium Bioorganische Chemie. Dortmund (D), 2007.}} @misc{neffe_rational_optimization_2007, author={Neffe, A.T., Bilang, M., Grueneberg, I., Meyer, B.}, title={Rational Optimization of the Binding Affinity of CD4 Targeting Peptidomimetics With Potential Anti HIV Activity}, year={2007}, howpublished = {journal article}, doi = {https://doi.org/10.1021/jm070206b}, abstract = {We recently reported the design and synthesis of a CD4 binding peptidomimetic with potential as HIV entry inhibitor. Variation of side chains and amino terminus provided first structure-activity relationships and confirmed the activity of the compounds as well as the correctness of our approach [Neffe, A. T.; Bilang, M.; Meyer, B. Org. Biomol. Chem. 2006, 4, 3259-3267]. Here we describe optimizations at the carboxy terminus of the peptidomimetic CD4 ligands resulting in the highest binding affinity of KD = 6 M for compound 4 determined with surface plasmon resonance (SPR). Saturation transfer difference NMR experiments with two peptidomimetics give binding constants similar to the SPR experiments and verified the ligand binding epitope. The higher proteolytic stability of the peptidomimetics compared to the lead peptide is demonstrated in a pronase digestion assay. Comparison of modeling and analytical data shows good agreement of theoretical and practical experiments.}, note = {Online available at: \url{https://doi.org/10.1021/jm070206b} (DOI). Neffe, A.; Bilang, M.; Grueneberg, I.; Meyer, B.: Rational Optimization of the Binding Affinity of CD4 Targeting Peptidomimetics With Potential Anti HIV Activity. Journal of Medicinal Chemistry. 2007. vol. 50, no. 15, 3482-3488. DOI: 10.1021/jm070206b}} @misc{abell_investigation_into_2007, author={Abell, A.D., Jones, M.A., Neffe, A.T., Aitken, S.G., Cain, T.P., Payne, R.J., McNabb, S.B., Coxon, J.M., Stuart, B.G., Pearson, D., Lee, H.Y.-Y., Morton, J.D.}, title={Investigation into the P3 Binding Domain of m-Calpain Using Photoswitchable Diazo- and Triazene-dipeptide Aldehydes: New Anticataract Agents}, year={2007}, howpublished = {journal article}, doi = {https://doi.org/10.1021/jm061455n}, abstract = {The photoswitchable N-terminal diazo and triazene-dipeptide aldehydes 8a-d, 10a,b, and 17a,b present predominantly as the (E)-isomer, which purportedly binds deep in the S3 pocket of calpain. All compounds are potent inhibitors of m-calpain, with 8b being the most active (IC50 of 35 nM). The diazo-containing inhibitors 8a, 8c, and 10a were irradiated at 340 nm to give a photostationary state enriched in the (Z)-isomer, and in all cases, these were less active. The most water soluble triazene 17a (IC50 of 90 nM) retards calpain-induced cataract formation in lens culture.}, note = {Online available at: \url{https://doi.org/10.1021/jm061455n} (DOI). Abell, A.; Jones, M.; Neffe, A.; Aitken, S.; Cain, T.; Payne, R.; McNabb, S.; Coxon, J.; Stuart, B.; Pearson, D.; Lee, H.; Morton, J.: Investigation into the P3 Binding Domain of m-Calpain Using Photoswitchable Diazo- and Triazene-dipeptide Aldehydes: New Anticataract Agents. Journal of Medicinal Chemistry. 2007. vol. 50, no. 12, 2916-2920. DOI: 10.1021/jm061455n}} @misc{neffe_bioaktive_peptide_2007, author={Neffe, A.T.}, title={Bioaktive Peptide und Glycokonjugate - HIV-Entry-Inhibitoren, Calpain-Inhibitoren und Biomimetik}, year={2007}, howpublished = {conference lecture (invited): Berlin (D);}, note = {Neffe, A.: Bioaktive Peptide und Glycokonjugate - HIV-Entry-Inhibitoren, Calpain-Inhibitoren und Biomimetik. Wissenschaftliches Kolloquium am Zentralinstitut fuer Laboratoriumsmedizin und Pathobiochemie. Berlin (D), 2007.}} @misc{neffe_modelling_und_2007, author={Neffe, A.T.}, title={Modelling und Synthese von peptidomimetischen Wirkstoffen -HIV-Entry-Inhibitoren und Calpain Inhibitoren}, year={2007}, howpublished = {conference lecture: Potsdam (D);}, note = {Neffe, A.: Modelling und Synthese von peptidomimetischen Wirkstoffen -HIV-Entry-Inhibitoren und Calpain Inhibitoren. GDCh-Vortrag Ortsverband Potsdam. Potsdam (D), 2007.}} @misc{kim_bayes_mix_2006, author={Kim, S.W., Hudson, I.L., Neffe, A.T., Abell, A.D.}, title={Bayes Mix Identification of Important Docking Parameters, Exemplified for Calpain Inhibitors}, year={2006}, howpublished = {conference lecture: Canberra (AUS);}, note = {Kim, S.; Hudson, I.; Neffe, A.; Abell, A.: Bayes Mix Identification of Important Docking Parameters, Exemplified for Calpain Inhibitors. Bioinformatics Summer Symposium THEME, Recent Discoveries and New Challenges. Canberra (AUS), 2006.}} @misc{neffe_calpain_inhibitoren_2006, author={Neffe, A.T.}, title={Calpain Inhibitoren zur Behandlung von Katarakten}, year={2006}, howpublished = {conference lecture: Rostock (D);}, note = {Neffe, A.: Calpain Inhibitoren zur Behandlung von Katarakten. Seminar der Universitaets-Augenklinik. Rostock (D), 200